Berberine-containing serum-free medium for mesenchymal stem cells

Abstract

The invention provides berberine-containing serum-free medium for mesenchymal stem cells, comprising a DMEM basic medium and further comprising, by concentration, 5-110mg/L berberine, 3-20mg/ml basic fibroblast growth factors, 3-20mg/ml epidermal growth factors, 1-10Mug/mL glutathione, 1-10mg/ml recombinant human insulin, 5-15mg/ml human serum albumin, 1-10Mug/mL transferrin, 5-15mg/L fibronectin, 5-30mg/L putrescine, 0.37-4mg/L aminoethanol, 10-50Mug/mL hydrocortisone, and 0.02-0.2mg/ml sodium pyruvate. The invention belongs to the technical field of stem cells; the medium has good cell adherence, high cell proliferation rate and capable of delaying decline phase of MSCs (mesenchymal stem cells) and prolong growth period of the cells.

Description

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AI Technical Summary

Benefits of technology

This new type of media called Serums (serum) without any animals' blood contains many valuable ingredients like fibers that help it grow healthy tissues quickly on Earth. These materials include hyaluronan glycosaminogens extracted from bovines skullcap root extractives, lyophilized yeast protein hydrogel, lithium chloride crystal powders, ellagic acid peptides, flavonoids, polyphenols, carboxylate esterase enzyme catalysts, etc., along with other compounds found naturally within plants such as polysaccharides, amino acids, sugars, nucleotides, nucleic acids and lipids. They work together to protect these important molecules during their lifetimes inside living organisms while still being able to perform various functions necessary for maintaining life activities.

Problems solved by technology

This technical problem addressed in this patented text relates to improving the quality control and reproduction efficiency of cultured mammals' own or foreign embryonic germ layers without compromising their healthy status. Current methods involve complicated processes like enzyme treatment, high cost, and lack precision. There exists a new method called magnetically induced plasma transformation (MAPT), where magnetism helps creature tiny structures inside the body while maintaining its stability. However, current technologies suffer from various drawbacks including difficulty obtaining pure samples due to impurity content, long preparations process cycles, limited cellular attachment, etc., making them difficult to use clinically because they may cause damage when administered outside the laboratory environment.

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