77results about How to "Does not cause immune rejection" patented technology

The invention discloses a vascular stent material of tissue engineering and manufacturing method thereof, which comprises selecting acetobacter xylinum as a model strain; using oxygen permeable materials to prepare hollow tubular dies which are arranged in the fermentation liquid and introduced with oxygen for fermentation until the bacterial celluloses completely cover the outer surface of the dies and the required porosity, thickness, uniformity and structural characteristic are reached to obtain a vascular stent raw material of tissue engineering; removing impurities to reduce the impurity rate to below 0.05%, sterilizing and packaging to prepare the vascular stent material of tissue engineering. The invention is simple in process and safe and has no toxic and side effect, and the material has more uniform spatial structure, more suitable porosity, uniform texture of each material in batches by controlling the fermentation process to effectively improve the quality and favourably achieve the scaled production.

The invention discloses a method for preparing a decellularized lamellar cornea matrix sheet. The method comprises the steps of disinfecting a fresh animal eyeball; treating with filter paper dipped with alcohol after disinfecting, and then erasing an epithelial cell layer; incising the corneal limbus under an operation microscope, stretching into an iris restorer to separate the anterior cornea, and then shearing the anterior cornea with corneal scissors; drilling the lamellar cornea with a corneal annulus; soaking the fresh lamellar cornea into serum, and then rinsing or performing ice-bath electrophoresis treatment; performing gradient dehydration after the treatment to obtain a non-dried lamellar tissue engineering corneal frame, sterilizing with ethylene oxide, and preserving for later use; drying the non-dried cornea matrix sheet in a 24-pore plate to obtain dried decellularized lamellar cornea; and sterilizing the dried sample with cobalt 60, and performing rehydration treatment to obtain a rehydrated lamellar cornea matrix sheet. The obtained cornea matrix sheet is high in transparency, low in structural destroy, good in biocompatibility, close to fresh cornea in performance and thorough in decellularization.

The invention discloses application of an extracellular matrix freeze-drying powder. The extracellular matrix freeze-drying powder is prepared from fresh mammal tissues by rinsing, disinfecting, degreasing, rinsing, cell removal, cleaning, pulping, extracting and freeze-drying. The extracellular matrix freeze-drying powder has the advantages that the multiple active matters of polytype collagen, proteoglycan, growth factors and the like in the animal tissues are reserved; the multiple biological activities are realized, the biocompatibility is good, and the tissue regeneration is promoted; theanti-oxidizing, repair and aging-resisting effects are realized, and the proliferation, transfer and differentiation of the cells are induced; the extracellular matrix freeze-drying powder can be used as the structure support, and the filling function is realized.