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Recombinant human type III collagen, expression strain and construction method

A collagen and construction method technology, applied in the field of Pichia pastoris engineering bacteria and its construction, can solve problems such as hidden dangers of animal viruses, complex properties, difficult processing, etc., and achieve the effects of good hydrophilicity and stability

Active Publication Date: 2020-07-03
JIANGSU JLAND BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because natural collagen is not easy to dissolve in water, and the collagen used is usually bovine or pig origin, the molecular weight is different, the properties are very complex, it is difficult to carry out further processing, and there are hidden dangers of animal viruses, which limits its use as a biological material in medical devices. applications in other fields

Method used

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  • Recombinant human type III collagen, expression strain and construction method
  • Recombinant human type III collagen, expression strain and construction method
  • Recombinant human type III collagen, expression strain and construction method

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Embodiment Construction

[0020] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings. The process, conditions, reagents, experimental methods, etc. for implementing the present invention are general knowledge and common knowledge in the art except for the content specifically mentioned below, and the present invention has no special limitation content.

[0021] 1. Protein sequence selection

[0022] The amino acid sequence (SEQ No.1) of human type III α-chain collagen was analyzed for hydrophobicity, and the evaluation results were as follows: figure 1 shown. The lower the hydrophobicity evaluation score, the better the hydrophilicity. Amino acid fragments with low scores are selected, and these fragments are integrated into a new protein, that is, the recombinant human type III collagen of the present invention (SEQ No.2). Hydrophobic analysis of the amino acids of recombinant human type III collagen, the results are as follo...

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Abstract

The invention discloses a recombinant human type III collagen protein, an expression strain and a construction method. The recombinant human type III collagen contains 498 amino acids and has a theoretical molecular weight of about 54.5kd. The constructed recombinant human type III collagen expression strain can effectively, stably and largely express the recombinant human type III collagen. The recombinant human collagen has good hydrophilicity and stability, the structure is 100% identical to the corresponding part of a natural collagen gene sequence, immune rejection does not caused when the recombinant human collagen is used in a human body, and the recombinant human collagen can be widely used in fields such as biomedical materials and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a recombinant human type III collagen, a Pichia engineering bacterium expressing the recombinant human type III collagen and a construction method thereof. Background technique [0002] Collagen is the most important structural protein in vertebrates, accounting for about 25-30% of the total protein. As the most abundant protein in the extracellular matrix, collagen is the most important structural protein in connective tissue and almost all parenchyma interstitial tissues, and plays a role in stabilizing tissues and organs and maintaining their structural integrity. Because natural collagen is not easily soluble in water, and the collagen used is usually bovine or pig origin, the molecular weight is different, the properties are very complex, it is difficult to carry out further processing, and there are hidden dangers of animal viruses, which limits its use as a biological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12N15/12C12N15/81C12N1/19C12R1/84
CPCC07K14/78C12N15/815
Inventor 赵健烽高力虎冯丽萍黄建民
Owner JIANGSU JLAND BIOTECH CO LTD
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