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Bioactive polypeptide QEPVL, and preparation and application thereof

A technology of biologically active peptides and derivatives, applied in the field of protein, can solve problems such as human body risks, achieve the effects of improving immunity, enhancing proliferation ability, and reducing damage

Active Publication Date: 2013-03-13
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • Bioactive polypeptide QEPVL, and preparation and application thereof
  • Bioactive polypeptide QEPVL, and preparation and application thereof
  • Bioactive polypeptide QEPVL, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1 active peptide QEPVL

[0057] 1. Preparation of fermented milk

[0058] 1) Lactobacillus helveticus fermented milk

[0059] Use skimmed milk powder (New Zealand NZMP brand skimmed milk powder) and water to prepare 12wt% skimmed milk (12g skimmed milk powder is added to 88g water, the same below). Under aseptic conditions, the tricyclic colony of Lactobacillus helveticus (CICC6024) was picked, added into sterilized 12wt% skim milk, and stirred evenly under aseptic conditions. After inoculation, seal with aluminum foil to prevent contamination. Place in an incubator at 37°C for 19 hours. After the cultivation is over, the curd is stirred evenly under aseptic conditions to complete the activation of the Lactobacillus helveticus and prepare the starter for preparing the Lactobacillus helveticus fermented milk.

[0060] Inoculate 10mL of the prepared Lactobacillus helveticus starter into 500mL of sterilized 12wt% skim milk (inoculation rat...

Embodiment 2

[0131] Preparation and Confirmation of Example 2 Bioactive Peptide QEPV

[0132] 1. Enzymatic hydrolysis of bioactive peptide QEPVL by simulating gastrointestinal digestion in vitro

[0133] The simulated gastrointestinal digestion experiment is mainly divided into two steps. First, use sterilized deionized water to prepare a bioactive peptide QEPVL solution with a concentration of 500 μg / mL, and add pepsin (purchased from Sigma Company) to the QEPVL solution with a concentration of 500 μg / mL at a ratio of 20 mg of pepsin per gram of QEPVL. The pH value of the reaction solution was brought to 2.0, and incubated in a constant temperature water bath at 37°C for 90 minutes; then the pH value of the reaction solution was adjusted to 7.5, and trypsin (Corolase PP, purchased from AB Company in Germany) was added at the ratio of adding trypsin per gram of QEPVL 40 mg, kept in a constant temperature water bath at 37°C for 150 minutes; finally placed in a water bath at 95°C for 5 minu...

Embodiment 3

[0148] Antioxidant activity experiment of embodiment 3 bioactive polypeptide QEPVL and QEPV

[0149] The antioxidant activity of the biologically active polypeptide QEPVL obtained in Example 1 was tested by free radical scavenging method (DPPH·method) and total antioxidant capacity method (Ferric ReducingAbility Power FRAP method).

[0150] 1. Determination of in vitro antioxidant activity of bioactive peptide QEPVL by [DPPH·] method

[0151] 1) Experimental reagents and instruments

[0152] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 The milk-derived biologically active polypeptide QEPVL (collected peak D sample) obtained from the fermentation of Lactobacillus helveticus and the milk-derived biologically active polypeptide QEPV obtained in Example 2.

[0153] Main instruments: Sunrise microplate reader, product of Tecan Compa...

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Abstract

The invention relates to the field of a protein and particularly relates to a milk-derived bioactive polypeptide QEPVL with in-vitro antioxidant activity and body immunity promoting activity, wherein an amino acid sequence of the bioactive polypeptide QEPVL is Gln-Glu-Pro-Val-Leu. Through an in-vitro antioxidant test and an in-vitro immunity promoting test, the polypeptide QEPVL is proved to have relatively good antioxidant biological activity and immunity improving function; on one hand, free radicals in the body can be removed to reduce the harm on the human body caused by the free radicals; on the other hand, according to the bioactive polypeptide QEPVL, the immunity of the body can be further enhanced and the multiplication capacity of lymphocytes in vitro can be promoted, thereby improving the ability of the body to resist infections of external pathogens and reducing the incidence rate of the body without causing immunological rejections. The bioactive polypeptide QEPVL has great significance for the development of milk products, healthcare products and medicines with the antioxidant function and the immunity enhancement.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide QEPVL and its preparation and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] Oxidation reaction and oxidative metabolism are crucial for food and human body, and free radicals and reactive oxygen species cause a series of oxidation reactions. When excessive free radicals are formed, they will exceed the protective effect o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N15/12C12P21/02A23L1/305A61K38/08A61P37/04A61P39/06C12R1/225
CPCC12P21/06A61K38/08C07K1/20C07K1/36C12R1/225A61K38/018C07K7/06C07K1/34A23L1/305C12P21/02C07K14/4732A23L33/18A23L33/19A61P37/04A61P39/06
Inventor 张少辉卢姗姗马鎏镠孙冠华崔磊余芳周婕慧
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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