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Application of extracellular matrix freeze-drying powder

A technology of exogenous matrix and freeze-dried powder, which is applied in preparations for toiletry, preparations for skin care, medical science, etc., can solve problems such as easy displacement and wear, and difficulty in shaping, so as to maintain integrity and solve dryness. , enhance the effect of circulation

Inactive Publication Date: 2018-11-16
广州昕生医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since autologous tissue comes from itself, it generally does not cause rejection and sensitization, but it is difficult to shape in the production process
Materials such as solid silicone rubber are non-toxic and harmless, and have relatively stable tissue compatibility. They are the main materials for medical prosthesis rhinoplasty. They are easy to shape during the production process, but silicone rubber implants are prone to displacement and wear and tear

Method used

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  • Application of extracellular matrix freeze-drying powder
  • Application of extracellular matrix freeze-drying powder
  • Application of extracellular matrix freeze-drying powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] An embodiment of the freeze-dried extracellular matrix powder provided by the present invention, the preparation method of the freeze-dried extracellular matrix powder comprises the following steps:

[0059] (1) Take the porcine small intestine tissue, wash after defatting and decellularization, and obtain the crude extracellular matrix extract;

[0060] (2) Mix the acid protease with a concentration of 0.01% and the crude extract of the extracellular matrix at a weight ratio of 1:20, shake for 36 hours, centrifuge to take the supernatant, use a dialysis bag with a molecular weight cut-off of 50,000Da, and dialyze with running water for 24 hours , until the conductivity is 1.5um / s, the extracellular matrix extract is obtained;

[0061] (3) Filter the extracellular matrix extract obtained in step (3) using a 0.8 μm microporous membrane, mix the filtrate with mannitol, and make the concentration of mannitol in the mixed solution 10%, and the mixed solution is heated at -2...

Embodiment 2

[0063] An embodiment of the freeze-dried extracellular matrix powder provided by the present invention, the preparation method of the freeze-dried extracellular matrix powder comprises the following steps:

[0064] (1) Take porcine pericardium tissue, wash after degreasing and decellularization to obtain crude extracellular matrix extract;

[0065] (2) Mix pepsin with a concentration of 1% and the crude extracellular matrix extract according to a weight ratio of 1:5, shake for 12 hours, filter the filtrate, add ethanol to the filtrate to make the final concentration 60%, Stir evenly, and obtain the extracellular matrix extract after standing still for precipitation;

[0066] (3) Centrifuge the extracellular matrix extract obtained in step (3), mix the supernatant with glucose so that the concentration of glucose in the mixed solution is 0.2%, and freeze the mixed solution at -20°C for 3 hours, and then freeze it at - Freeze-dry at 80° C. for 12 hours to obtain the freeze-drie...

Embodiment 3

[0068] An embodiment of the freeze-dried extracellular matrix powder provided by the present invention, the preparation method of the freeze-dried extracellular matrix powder comprises the following steps:

[0069] (1) Take the bovine bladder tissue, degrease and wash after decellularization to obtain the crude extracellular matrix extract;

[0070] (2) Mix the neutral protease with a concentration of 0.1% and the crude extract of the extracellular matrix according to a weight ratio of 1:10, after shaking for 24 hours, centrifuge to get the supernatant, add sodium chloride to the supernatant to make it The final concentration is 0.5mol / L, stir evenly, and obtain the extracellular matrix extract after standing for precipitation;

[0071] (3) Filter the extracellular matrix extract obtained in step (3) with a 0.8 μm microporous membrane, mix the filtrate with trehalose, and make the concentration of trehalose in the mixed solution 1%, and the mixed solution is heated at -20°C F...

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Abstract

The invention discloses application of an extracellular matrix freeze-drying powder. The extracellular matrix freeze-drying powder is prepared from fresh mammal tissues by rinsing, disinfecting, degreasing, rinsing, cell removal, cleaning, pulping, extracting and freeze-drying. The extracellular matrix freeze-drying powder has the advantages that the multiple active matters of polytype collagen, proteoglycan, growth factors and the like in the animal tissues are reserved; the multiple biological activities are realized, the biocompatibility is good, and the tissue regeneration is promoted; theanti-oxidizing, repair and aging-resisting effects are realized, and the proliferation, transfer and differentiation of the cells are induced; the extracellular matrix freeze-drying powder can be used as the structure support, and the filling function is realized.

Description

technical field [0001] The invention relates to the use of a freeze-dried extracellular matrix powder, in particular to the use of a freeze-dried extracellular matrix powder with multiple biological activities and good biocompatibility. Background technique [0002] With the continuous deterioration of the living environment of human beings, the human body has to bear the damage from the outside world every day. Ultraviolet rays, exhaust gas pollution, smog, life stress, etc. can all cause cell damage. At the same time, with the improvement of people's living standards, people pay more and more attention to the quality of life, such as the demand for physical health and medical cosmetology. [0003] There are five main types of skin problems caused by environmental pollution, dryness, spots, acne, wrinkles, and sensitivity. 1. Dryness: less sebum secretion, dry skin, lack of luster, prone to wrinkles, peeling, and tight feeling. The main reason is that the water content i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61Q19/00A61Q19/02A61Q19/08A61K35/12A61P17/02A61L15/40A61L15/44A61L26/00
CPCA61K8/981A61K35/12A61L15/40A61L15/44A61L26/0057A61L26/0066A61L2300/30A61L2300/412A61Q19/00A61Q19/02A61Q19/08A61P17/02
Inventor 杨伟红耿梦梦姜玉峰程飚苏玉天刘江辉陈妮丹陈大鹏邓小敏
Owner 广州昕生医学材料有限公司
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