31results about How to "Induced regeneration" patented technology

A new polymeric material for biological tissue regeneration or treatment with a drug delivery system which contains therapeutic agents and / or bioactive molecules to reduce infection and inflammatory reaction at a wound site and maximize tissue regeneration and wound healing is provided. The new bioactive molecule-loaded polymeric material is prepared by (1) preparing micrometer or nanometer sized bioactive molecule-loaded particles; (2) modifying the surface of the prepared particles and immobilizing the particles on the surface of the polymeric material; and (3) physically treating the surface of the polymeric material to improve binding strength of the particles immobilized thereon.

The present invention provides a preparation method of high-strength bionic collagen membrane. The preparation method is by conducting gradient dialysis process on collagen to rearrange collagen molecules into an ordered structure, and endow the molecules with more consistent and more extensive collagen fiber orientation, thus improving the mechanical properties of the material. The collagen membrane prepared by using the method has similar appearance, structure and performance to natural dura and amnion of mammals, and good rehydration, and is free to bend and fold after water absorption; the bionic collagen membrane with high tensile strength can be stitched by using suture and can be used in clinical guided tissue regeneration or repair of various tissues.

Bioactive implant for myocardial regeneration and ventricular chamber support including an elastomeric microporous membrane. The elastomeric microporous membrane being at least one non-degradable polymer and at least one partially degradable polymer. The non-degradable polymer is selected from polyethylacrylate and polyethylacrylate copolymerized with a hydroxyethylacrylate comonomer. The partially degradable polymer is selected from caprolactone 2-(methacryloyloxy)ethyl ester and caprolactone 2-(methacryloyloxy)ethyl ester copolymerized with ethylacrylate. The elastomeric microporous membrane further includes a nanofiber hydrogel, and cells. The bioactive implant, having one or two helical loops, contributes to the restauration of the heart conical shape. Cardiac wrapping by ventricular support bioprostheses of the present invention, having reinforcement bands spatially distributed as helicoids, recovers the sequential contraction of the myocardium resulting in the successive shortening and lengthening of the ventricles, therefore improving the ejection (systolic function) and suction of blood (diastolic function).

The present invention discloses a method for regenerating nerve fibers of a subject comprising the steps of: (a) selecting at least a target portion of the nervous system of a subject with a lesion in the nervous system; (b) detecting electromagnetic wave profile of said at least a target portion of said nervous system of said subject; (c) comparing wave profiles of said subject with wave profiles of normal patients; and (d) defining a treatment protocol. According to a certain aspect, the step of defining said treatment protocol includes selecting compensating electromagnetic waves adapted for correcting anomalous wave profiles associated with said nervous system lesions and transmitting to said subject said protocol of compensating electromagnetic waves to provide a resonance effect; thereby inducing regeneration of nerve fibers at said nervous system lesions and regions proximate to said lesions. The present invention further discloses apparatus and a computer implemented method thereof.

The invention relates to a tissue engineering meniscus repair sheet and a preparation method thereof. The prepared tissue engineering meniscus repair sheet comprises an acellular meniscal thin sheet, seed cells and growth factor slow-release carriers, wherein blind micro vias are uniformly distributed on two surfaces of the acellular meniscal thin sheet, and the seed cells and the growth factor slow-release carriers are combined on two surfaces of the acellular meniscal thin sheet. The blind micro vias distributed on the acellular meniscal thin sheet increase the area of a support material, and can be used as a storage pool of the seed cells and the growth factor slow-release carriers, so that the seed cells and the growth factor slow-release carriers can be firmly attached and are not easy to fall off. Nutrition can be persistently released through the slow-release carrier by multiple growth factors, the cell amplification efficiency is improved, the cell phenotype is also maintained, cell ageing is inhibited, and fusion of the tissue engineering meniscus repair sheet and self tissue is promoted. The prepared tissue engineering meniscus repair sheet has the structure characteristic similar to the structure characteristic of natural meniscus, and is applicable to repair meniscus injure and induce regeneration of meniscus fibrous cartilage tissue.

Disclosed is a method for producing exosomes having effects of skin regeneration and wound healing, comprising the steps of: providing ultrasonic stimulation directly or indirectly to cells; culturinga mixture of the cells and a medium for a predetermined amount of time; and separating exosomes from the mixture, wherein direct provision of stimulation is the application of ultrasonic stimulationto a medium comprising cells, and indirect provision of stimulation is the application of ultrasonic stimulation to a medium that does not comprise cells and then mixing the medium with the cells. Theexosome production method enables exosomes, having effects of skin regeneration and wound healing, to be obtained in a high yield and short amount of time by means of a simple process called sonication from stem cells, which are difficult to be handled, and also from somatic cells, which can be easily obtained and maintained. The exosomes obtained thereby also have a high amount and number of various included factors, the exosomes and compositions comprising same have increased various factors, which can induce skin regeneration and wound healing, and the exosomes have a phospholipid-based membrane structure so as to facilitate skin penetration and allow high efficiency of substance delivery, thereby enabling skin regeneration and wound healing to be effectively induced, and are safe andhave no side effects caused by the use of synthetic drugs.

The invention relates to a method for preparing an artificial microenvironment and application thereof. Specifically, the method comprises the steps that firstly, a solid-phase carrier is inoculated with functional cells; secondly, the solid-phase carrier obtained in the first step is kept for preset time under the condition which is suitable for growing of the functional cells; thirdly, the solid-phase carrier obtained in the second step is subjected to de-cellularization treatment so that the artificial microenvironment can be obtained. By means of the method, the artificial microenvironment which has the advantages that low immunogenicity and good controllability are achieved, the quality is stable, the production cycle is short and components are controllable and can achieve customization can be prepared.

The invention discloses a diagnosis and treatment integrated gradient osteochondral bionic scaffold and a preparation method thereof, which adopts a DLP photocuring 3D printing technology, and the preparation method comprises the following steps: respectively preparing sol of a cartilage layer, a cartilage calcification layer and a subchondral bone layer, and preparing cartilage layer sol by utilizing quercetin, methacrylate esterified gelatin and an LAP solution; preparing cartilage calcification layer sol by utilizing quercetin, methacrylate esterified gelatin, iron-doped hydroxyapatite andan LAP solution; preparing subchondral bone layer sol by utilizing quercetin, methacrylate esterified gelatin, iron-doped hydroxyapatite and an LAP solution. The scaffold prepared by the method has structural gradient and material gradient, has good mechanical properties and biocompatibility, and has good bone induction capability and bone conduction capability, which can induce regeneration of bone tissue defect parts. The gradient scaffold provided by the invention has an MRI diagnosis function, which can be used to accurately evaluate the real-time condition of osteochondral defect repair in vivo.

The present invention provides a method for preparing collagen and bioceramic powder composite material microparticles, said method includes the following steps: mixing collagen solution, bioceramic powder and alginic acid, adopting dropping mode to make the above-mentioned mixture drop into the bivalent cationic aqueous solution to peptize and form microparticle form, using chitosan solution to make treatment, liquifying to wash out internal alginic acid and chitosan from surface, at the same time making collagen implement recombination. Said invented microparticle composite material possesses the component similar to bont tissue and collagen fiber recticular formation, can provide cell growth environment similar in bone tissue, can be used as carrier to carry cell and cover and fix various bone growth factors, can be used for repairing bont tissue wound.

The invention discloses application of an extracellular matrix freeze-drying powder. The extracellular matrix freeze-drying powder is prepared from fresh mammal tissues by rinsing, disinfecting, degreasing, rinsing, cell removal, cleaning, pulping, extracting and freeze-drying. The extracellular matrix freeze-drying powder has the advantages that the multiple active matters of polytype collagen, proteoglycan, growth factors and the like in the animal tissues are reserved; the multiple biological activities are realized, the biocompatibility is good, and the tissue regeneration is promoted; theanti-oxidizing, repair and aging-resisting effects are realized, and the proliferation, transfer and differentiation of the cells are induced; the extracellular matrix freeze-drying powder can be used as the structure support, and the filling function is realized.

The present invention provides a method for improving pancreatic function in a subject in need thereof, the method comprising administering to the subject STRO-1+ cells and / or progeny cells thereof and / or soluble factors derived therefrom. The method of the invention is useful for treating and / or preventing and / or delaying the onset or progression of a disorder resulting from or associated with pancreatic dysfunction, e.g., resulting from abnormal endocrine or exocrine function of the pancreas.

Non-activated tissue-regeneration polypeptides (TRPs) and their preparation methods are disclosed. The TRPs include: a protein transduction domain (PTD) making the polypeptides to permeate a cell membrane without cell membrane receptors; a furin activation domain (FAD) which has at least one proprotein convertase cleavage site and which can be cleaved by the proprotein convertase and activate a tissue regeneration domain (TRD) in cells; and a tissue regeneration domain (TRD) which can be activated by the proprotein convertase cleavage of the FAD to stimulate the growth or formation of tissues or to induce the regeneration of tissues. The TRPs can be mass-produced by cultured bacteria, such as recombinant E. coli, are in a non-activated state before in vivo administration, and their separation, purification, handling, storage and administration are simple and convenient. The in vivo administration of the TRPs is useful to stimulate the formation or regeneration of tissues, such as bones or cartilages, or to improve the fibrosis and cirrhosis of organs, such as kidneys, liver, lungs and heart by pharmacological mechanisms completely different from those of prior rhBMPs or TGF-β proteins.

Non-activated tissue-regeneration polypeptides (TRPs) and the preparation methods thereof are disclosed. The TRPs contain: a protein transduction domain (PTD) making the polypeptides to permeate a cell membrane without cell membrane receptors; a furin activation domain (FAD) which has at least one proprotein convertase cleavage site and which can be cleaved by the proprotein convertase and activate a tissue regeneration domain (TRD) in cells; and a tissue regeneration domain (TRD) which can be activated by the proprotein convertase cleavage of the FAD to stimulate the growth or formation of tissues or to induce the regeneration of tissues. The TRPs can be practically mass-produced by the culture of bacteria, such as recombinant E. coli, and are in a non-activated state before in vivo administration. Thus, their production cost is only a few tenths of the prior active proteins having uses similar thereto, and processes for their separation, purification, handling, storage and administration are significantly simple and convenient. The in vivo administration of the TRPs can stimulate the formation or regeneration of tissues, such as bones or cartilages, or improve the fibrosis and cirrhosis of organs, such as kidneys, liver, lungs and heart by pharmacological mechanisims completely different from those of prior rhBMPs or TGF-β proteins. Accordingly, the TRPs will be useful as drugs having new mechanisms.

A tissue engineered meniscus repair sheet and a preparation method thereof. The tissue engineered meniscus repair sheet prepared by the present invention includes a decellularized meniscus sheet, seed cells and growth factor slow-release carriers, wherein the two surfaces of the decellularized meniscus sheet are uniform Micro blind holes are distributed, and seed cells and slow-release growth factor carriers are compounded on both surfaces of the decellularized meniscus sheet. The micro-blind pores distributed on the decellularized meniscus sheet increase the area of ​​the scaffold material, which can be used as a storage pool for seed cells and slow-release carriers of growth factors, making them firmly attached and not easy to fall off; The carrier can release nutrients for a long time, improve the efficiency of cell expansion, maintain cell phenotype, inhibit cell aging, and promote the fusion of tissue engineering meniscus repair sheet and its own tissue. The prepared tissue engineered meniscus repair sheet has structural characteristics similar to natural meniscus, and can be used for repairing meniscus injury and inducing meniscus fibrocartilage tissue regeneration.

Bioactive implant for myocardial regeneration and ventricular chamber support including an elastomeric microporous membrane. The elastomeric microporous membrane being at least one non-degradable polymer and at least one partially degradable polymer. The non-degradable polymer is selected from polyethylacrylate and polyethylacrylate copolymerized with a hydroxyethylacrylate comonomer. The partially degradable polymer is selected from caprolactone 2-(methacryloyloxy)ethyl ester and caprolactone 2-(methacryloyloxy)ethyl ester copolymerized with ethylacrylate. The elastomeric microporous membrane further includes a nanofiber hydrogel, and cells. The bioactive implant, having one or two helical loops, contributes to the restauration of the heart conical shape. Cardiac wrapping by ventricular support bioprostheses of the present invention, having reinforcement bands spatially distributed as helicoids, recovers the sequential contraction of the myocardium resulting in the successive shortening and lengthening of the ventricles, therefore improving the ejection (systolic function) and suction of blood (diastolic function).

The present invention provides a method for improving pancreatic function in a subject in need thereof, the method comprising administering to the subject STRO-1+ cells and / or progeny cells thereof and / or soluble factors derived therefrom. The method of the invention is useful for treating and / or preventing and / or delaying the onset or progression of a disorder resulting from or associated with pancreatic dysfunction, e.g., resulting from abnormal endocrine or exocrine function of the pancreas.

The invention discloses a hair growth micro-needle patch containing an androgen receptor protein targeted complex, which is characterized in that the hair growth micro-needle patch comprises a sheet-shaped backing and soluble micro-needle bodies which are arranged on the sheet-shaped backing and contain the androgen receptor protein targeted complex; the soluble microneedle body comprises one or more of an androgen receptor protein targeting complex, a pharmaceutically acceptable salt thereof, a stereoisomer thereof, a geometric stereomer thereof, a tautomer thereof, an ester thereof, a prodrug thereof, a solvate thereof, a metabolite thereof, a nitrogen oxide thereof or a deuterated compound thereof. The invention also discloses a preparation method of the hair-growing micro-needle patch containing the androgen receptor protein targeted complex and application of the hair-growing micro-needle patch in preparation of a medicine for treating androgen-derived alopecia. The hair-growing micro-needle patch provided by the invention has the advantages of safe effect, high efficiency and convenience in use.

The invention relates to a fat extract, a fat acellular matrix as well as a preparation method and application of the fat acellular matrix. The method comprises the following steps: cleaning and centrifuging fat tissue, mixing middle-layer fat tissue with PBS, and transferring the mixture into an injector (I), wherein the volume ratio of the middle-layer fat tissue to the buffer salt solution is (1 + / -0.2): 1; butting the injector (1) and an injector (2) through a joint, pushing a piston of the injector (1), continuously pushing and injecting back and forth to obtain slurry, and centrifuging to obtain fat extract and middle-layer fat tissue; and carrying out freezing-unfreezing cycle treatment, enzyme treatment and disinfectant treatment on the middle-layer fat tissue to obtain the fat acellular matrix. The invention provides a preparation method of a fat extract and a fat acellular matrix. The method is rapid and efficient, and the fat extract containing growth factors and the fat acellular matrix highly retaining natural extracellular matrix components and structures can be prepared.

Disclosed is a method for treating olfactory disorders, which comprises administering to a patient a therapeutically effective amount of 3-β-hydroxymethylglutarate CoA (HMG-CoA) reductase, which has excellent effects of protecting and regenerating olfactory nerve.

The invention discloses a method for establishing a zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration. The method is the first one for establishing an embryogenic cell suspension culture system on a zephyranthes candida plant. The highest embryonic callus induction rate can be 45.2 percent; the embryogenic cell suspension culture system is rapid in multiplication, the fresh weight of embryogenic cells can be multiplied by 2.9 times to the highest extent each week, and the highest plant regeneration rate of the embryogenic cells can be 85.3 percent.

The invention relates to a method of inducing osteogenesis in a subject comprising administrating a chitosan material to the a subject in need of osteogenesis, wherein the chitosan material having a chitosan with a degree of deacetylation at the range of 70%˜90%, and the chitosan is 0.15% by weight of the chitosan material. The method of the present invention can induce bone-forming and promote osseointegration around an implant.

The present invention provides a preparing method of a surface-modified microporous metal implant. The preparing method comprises providing a microporous metal implant with a plurality of anode ports; connecting the anode ports with positive poles of power sources while connecting an electrolytic bath with negative poles of the power sources; and immersing the microporous metal implant into an electrolyte, and performing micro-arc oxidation treatment through power supply of the power sources. Through arrangement of the anode ports, the electric current density of the microporous metal implant is controlled to be uniform through arrangement of the positions of the anode ports, and the field energy is distributed uniformly. In comparison with a conventional single anode port, the multiple anode ports can help realize a field energy distribution as uniform as possible on the microporous metal implant, thus to reduce differences in electric field intensity and in field energy between an inner wall and an outer wall of a structural hole, thereby acquiring in-situ growth film layers with a better quality. For example, the thickness and thickness differences of the in-situ growth film layers can be controlled.

The invention relates to a method of inducing osteogenesis in a subject comprising administrating a chitosan material to the a subject in need of osteogenesis, wherein the chitosan material having a chitosan with a degree of deacetylation at the range of 70%˜90%, and the chitosan is 0.15% by weight of the chitosan material. The method of the present invention can induce bone-forming and promote osseointegration around an implant.

A method for producing exosomes comprises steps of: providing ultrasound stimulation directly or indirectly to cells; culturing a mixture of the cells and a medium for a predetermined time; and isolating exosomes from the mixture, wherein providing the stimulation directly to the cells comprises applying ultrasound stimulation to the medium containing the cells, and the providing the stimulation indirectly to the cells comprises applying ultrasound stimulation to the medium not containing the cells and then mixing the medium and the cells. This method for producing exosomes makes it possible to obtain exosomes having a hair regeneration effect not only from stem cells and progenitor cells that are difficult to isolate and multiply, but also from somatic cells that may be easily obtained and maintained, in high yield within a short time by ultrasound treatment that is a simple process.

The invention provides a temperature-sensitive hydrogel material combination and a preparation method and application thereof, belonging to the technical field of regenerative medicine. The temperature-sensitive gel material combination provided by the present invention includes SDF-1-loaded chitosan-hyaluronic acid solution and Apt19S-loaded chitosan-hyaluronic acid solution. The present invention aims to develop a thermosensitive injectable bilayer hydrogel loaded with biological factors related to cell migration, so as to promote the regeneration of periodontal tissue.

The invention provides a temperature-sensitive hydrogel material composition as well as a preparation method and application thereof, and belongs to the technical field of regenerative medicine. The temperature-sensitive gel material composition provided by the invention comprises a chitosan hyaluronic acid solution loaded with SDF-1 and a chitosan hyaluronic acid solution loaded with Apt19S. The invention aims to develop a temperature-sensitive injectable double-layer hydrogel loaded with related biological factors capable of promoting cell migration, and the hydrogel is used for promoting regeneration of periodontal tissues.

The present disclosure relates to a method for controlling regeneration of diesel particulate filter in construction machinery. The regeneration controlling method for the diesel particulate filter of construction machinery according to the present disclosure includes: a first active generation operation of actively regenerating a diesel particulate filter (DPF) according to a predetermined first active regeneration schedule when a quantity of soot inside the DPF within is a predetermined allowance section; and a second active regeneration operation of actively regenerating the diesel particulate filter according to a second active regeneration schedule, which is different from the first active regeneration schedule, according to the quantity of soot when the quantity of soot exceeds the allowance section.

Embodiments of the disclosure encompass methods and compositions using fibroblasts for stimulating regeneration in a first tissue site in an individual, comprising the step of administering at least one regenerative composition to a second tissue site, wherein the second tissue site comprises the same tissue type as the first tissue site in the individual. The first and second sites are at different locations in the individual, in particular embodiments. Particular embodiments comprise administering one or more compositions to an individual at a different anatomical site than the site that is in need, such as a joint.

The present invention provides a method for preparing collagen and bioceramic powder composite material microparticles, said method includes the following steps: mixing collagen solution, bioceramic powder and alginic acid, adopting dropping mode to make the above-mentioned mixture drop into the bivalent cationic aqueous solution to peptize and form microparticle form, using chitosan solution to make treatment, liquifying to wash out internal alginic acid and chitosan from surface, at the same time making collagen implement recombination. Said invented microparticle composite material possesses the component similar to bont tissue and collagen fiber recticular formation, can provide cell growth environment similar in bone tissue, can be used as carrier to carry cell and cover and fix various bone growth factors, can be used for repairing bont tissue wound.