31results about How to "Induced regeneration" patented technology

Disclosed is a method for producing exosomes having effects of skin regeneration and wound healing, comprising the steps of: providing ultrasonic stimulation directly or indirectly to cells; culturinga mixture of the cells and a medium for a predetermined amount of time; and separating exosomes from the mixture, wherein direct provision of stimulation is the application of ultrasonic stimulationto a medium comprising cells, and indirect provision of stimulation is the application of ultrasonic stimulation to a medium that does not comprise cells and then mixing the medium with the cells. Theexosome production method enables exosomes, having effects of skin regeneration and wound healing, to be obtained in a high yield and short amount of time by means of a simple process called sonication from stem cells, which are difficult to be handled, and also from somatic cells, which can be easily obtained and maintained. The exosomes obtained thereby also have a high amount and number of various included factors, the exosomes and compositions comprising same have increased various factors, which can induce skin regeneration and wound healing, and the exosomes have a phospholipid-based membrane structure so as to facilitate skin penetration and allow high efficiency of substance delivery, thereby enabling skin regeneration and wound healing to be effectively induced, and are safe andhave no side effects caused by the use of synthetic drugs.

The present invention provides a method for improving pancreatic function in a subject in need thereof, the method comprising administering to the subject STRO-1+ cells and / or progeny cells thereof and / or soluble factors derived therefrom. The method of the invention is useful for treating and / or preventing and / or delaying the onset or progression of a disorder resulting from or associated with pancreatic dysfunction, e.g., resulting from abnormal endocrine or exocrine function of the pancreas.

Non-activated tissue-regeneration polypeptides (TRPs) and their preparation methods are disclosed. The TRPs include: a protein transduction domain (PTD) making the polypeptides to permeate a cell membrane without cell membrane receptors; a furin activation domain (FAD) which has at least one proprotein convertase cleavage site and which can be cleaved by the proprotein convertase and activate a tissue regeneration domain (TRD) in cells; and a tissue regeneration domain (TRD) which can be activated by the proprotein convertase cleavage of the FAD to stimulate the growth or formation of tissues or to induce the regeneration of tissues. The TRPs can be mass-produced by cultured bacteria, such as recombinant E. coli, are in a non-activated state before in vivo administration, and their separation, purification, handling, storage and administration are simple and convenient. The in vivo administration of the TRPs is useful to stimulate the formation or regeneration of tissues, such as bones or cartilages, or to improve the fibrosis and cirrhosis of organs, such as kidneys, liver, lungs and heart by pharmacological mechanisms completely different from those of prior rhBMPs or TGF-β proteins.

A tissue engineered meniscus repair sheet and a preparation method thereof. The tissue engineered meniscus repair sheet prepared by the present invention includes a decellularized meniscus sheet, seed cells and growth factor slow-release carriers, wherein the two surfaces of the decellularized meniscus sheet are uniform Micro blind holes are distributed, and seed cells and slow-release growth factor carriers are compounded on both surfaces of the decellularized meniscus sheet. The micro-blind pores distributed on the decellularized meniscus sheet increase the area of ​​the scaffold material, which can be used as a storage pool for seed cells and slow-release carriers of growth factors, making them firmly attached and not easy to fall off; The carrier can release nutrients for a long time, improve the efficiency of cell expansion, maintain cell phenotype, inhibit cell aging, and promote the fusion of tissue engineering meniscus repair sheet and its own tissue. The prepared tissue engineered meniscus repair sheet has structural characteristics similar to natural meniscus, and can be used for repairing meniscus injury and inducing meniscus fibrocartilage tissue regeneration.

The invention provides a temperature-sensitive hydrogel material combination and a preparation method and application thereof, belonging to the technical field of regenerative medicine. The temperature-sensitive gel material combination provided by the present invention includes SDF-1-loaded chitosan-hyaluronic acid solution and Apt19S-loaded chitosan-hyaluronic acid solution. The present invention aims to develop a thermosensitive injectable bilayer hydrogel loaded with biological factors related to cell migration, so as to promote the regeneration of periodontal tissue.

Described herein is a method for generating cardiomyocytes (CMs) from non-cardiac cells.

The present invention provides a method for preparing collagen and bioceramic powder composite material microparticles, said method includes the following steps: mixing collagen solution, bioceramic powder and alginic acid, adopting dropping mode to make the above-mentioned mixture drop into the bivalent cationic aqueous solution to peptize and form microparticle form, using chitosan solution to make treatment, liquifying to wash out internal alginic acid and chitosan from surface, at the same time making collagen implement recombination. Said invented microparticle composite material possesses the component similar to bont tissue and collagen fiber recticular formation, can provide cell growth environment similar in bone tissue, can be used as carrier to carry cell and cover and fix various bone growth factors, can be used for repairing bont tissue wound.