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Method for preparing collagen and biological ceramic powder composite material microparticles

A technology of bioceramics and collagen, which is applied in the field of preparing composite materials in the form of particles, can solve the problems of tissue damage and destruction of collagen properties, etc.

Inactive Publication Date: 2004-12-08
洪尧顺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of preparing microparticles by the above method, the use of organic solvents may destroy the properties of collagen, and the residual organic oil or cross-linking agent may cause damage to the tissue, and more procedures are required to separate and remove the oil phase

Method used

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  • Method for preparing collagen and biological ceramic powder composite material microparticles
  • Method for preparing collagen and biological ceramic powder composite material microparticles
  • Method for preparing collagen and biological ceramic powder composite material microparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Please refer to figure 1 As shown, the collagen and bioceramic powder composite particles were prepared according to the following steps:

[0030] 1. Take 6ml of the purified type I collagen solution (concentration: 6mg / ml), 66.86mg of β-tricalcium PhosPhate powder, and 1.8ml of alginic acid solution (concentration: 6%) and 1.2 ml of phosphate buffer saline (PBS, PhosPhatebuffer saline), which were thoroughly mixed. Wherein the weight percent ratio of collagen protein and β-tricalcium phosphate is 35 / 65.

[0031] 2. With the air Jet-syringe pump extrusion, adjust the nitrogen flow rate to 5ml / min, drop the above mixed solution into calcium chloride (CaCl 2 , 1.5%) gelled into microparticles in aqueous solution. The above process was performed at 4 °C to prevent collagen reorganization.

[0032] 3. The calcium chloride aqueous solution was replaced with Ca-CHES (2-N-cyclohexylaminoethane-sulfuric acid), and the particles were aged in it for 5 minutes for cleaning. T...

Embodiment 2

[0037] The device and steps are as in Example 1, except that in step 3, 0.005W / V% polyglucosamine solution (prepared with 0.005% lactic acid, pH value = 5.5) is coated for 5 minutes, and the others are the same as in Example 1 in the steps.

[0038] The results of this experiment show that more than 95% of the alginic acid has been washed out at 9 hours, and the alginic acid has been almost completely washed out after 12 hours. The structure of the particles was observed with a scanning electron microscope, Figure 3A and 3B at 9 hours, Figure 3C and 3D It is a SEM photo of particle section and surface structure observed at 10,000 times at 24 hours. The experimental results found that after 9 hours, the interior and surface of the particles had a three-dimensional collagen fiber structure, and there was a small amount of polyglucosamine residue on the surface; The sugars are almost all washed out along with the alginic acid.

Embodiment 3

[0040] The device and steps are as in Example 1, except that the ratio of collagen to β-tricalcium phosphate is changed to 25 / 75 in Step 1, and the others are the same as in Example 1. Experimental results (scanning electron micrographs show similar structures and forms as those shown in Examples 1 and 2) show that the microparticles have a three-dimensional collagen fiber network structure inside and on the surface. In the composite material particles, the higher the proportion of collagen, the more three-dimensional network fiber structure, but the particle strength is weaker and the cost is higher; if the proportion is lower, the strength is stronger and the cost is lower, but the three-dimensional network fiber structure is higher. Poor, unable to effectively provide an environment similar to the collagen fiber network structure in bone tissue for cell growth. Generally, the ratio of collagen to bioceramic powder is better between 10 / 90-90 / 10, and the ratio of 35 / 65 is the...

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Abstract

The present invention provides a method for preparing collagen and bioceramic powder composite material microparticles, said method includes the following steps: mixing collagen solution, bioceramic powder and alginic acid, adopting dropping mode to make the above-mentioned mixture drop into the bivalent cationic aqueous solution to peptize and form microparticle form, using chitosan solution to make treatment, liquifying to wash out internal alginic acid and chitosan from surface, at the same time making collagen implement recombination. Said invented microparticle composite material possesses the component similar to bont tissue and collagen fiber recticular formation, can provide cell growth environment similar in bone tissue, can be used as carrier to carry cell and cover and fix various bone growth factors, can be used for repairing bont tissue wound.

Description

field of invention [0001] The present invention relates to a method for preparing composite particles of collagen and bioceramic powder (bioceramic), in particular to a method for preparing composite materials in the form of particles, which have three-dimensional collagen fibers inside and on the surface grid. Background technique [0002] If there is a defect in human bone tissue due to injury or disease, it needs to be repaired to restore the function of the original tissue as much as possible. If bone resident plants are added to the defect as a scaffold to provide force and cell growth, it will effectively assist the structure and function of bone tissue. regeneration and restoration. Autologous transplantation is the best choice for bone implants, but its source is limited and suffers from secondary surgery; allograft or xenograft has immune and other complications; artificially synthesized bone tissue provides another new choose. The artificially synthesized implan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08K3/32C08L89/02
Inventor 王盈锦蔡晓雯黄秀萱张文忠
Owner 洪尧顺
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