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Diagnosis and treatment integrated gradient osteochondral bionic scaffold and preparation method thereof

An osteochondral and bionic technology, applied in scaffolds, prostheses, tissue regeneration, etc., can solve problems such as structural and functional changes in difficult organs, and achieve the promotion of osteoblast proliferation, good mechanical properties, biocompatibility, and high precision Effect

Active Publication Date: 2021-02-26
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, CT scanning is mostly used to evaluate the degradation of bionic osteochondral scaffolds. CT is a radiographic examination method, and it is difficult to find functional changes in organ tissue structures. In order to more comprehensively evaluate the degradation of bionic gradient osteochondral scaffolds and track and diagnose bone defects The repair of articular cartilage defects urgently needs an intuitive and non-invasive method for diagnosing the repair of articular cartilage defects.

Method used

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  • Diagnosis and treatment integrated gradient osteochondral bionic scaffold and preparation method thereof
  • Diagnosis and treatment integrated gradient osteochondral bionic scaffold and preparation method thereof
  • Diagnosis and treatment integrated gradient osteochondral bionic scaffold and preparation method thereof

Examples

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Embodiment 1

[0051] A method for preparing a gradient osteochondral bionic scaffold integrated with diagnosis and treatment, comprising the steps of:

[0052] (1) Establishment of osteochondral scaffold model: According to the principles of bionics and the size and structure of bone defects, use 3D modeling software to design a bionic bone scaffold model, and finally export the model to a 3D printer in STL format. The model in this example is: diameter 10mm, height 7mm The cylindrical gradient scaffolds are composed of cartilage layer, calcified cartilage layer and subchondral bone layer from top to bottom. The thickness of the lower bone layer is 3.5 mm, the pore diameter is 200-500 μm, and the pores are interconnected.

[0053] (2) Synthetic preparation of GelMA: Weigh 5g of gelatin with an analytical balance, dissolve it in 50mL of PBS solution (pH 7.4, 0.01M), and dissolve it completely at 60°C to obtain 50mL of gelatin solution, place the gelatin solution on Stir on a constant temper...

Embodiment 2

[0060] A method for preparing a gradient osteochondral biomimetic scaffold integrated with diagnosis and treatment, which is basically the same as in Example 1, except that the sol ratio of each layer is different:

[0061] Cartilage lamellar sol: Weigh quercetin and GelMA, dissolve and mix them evenly with LAP solution, the final concentrations of the two are 0.03% (w / w) and 18% (w / w) respectively to obtain cartilage lamellar sol, namely Example 2 cartilage lamellar sol.

[0062] Cartilage calcification layer sol: Weigh quercetin, GelMA and Fe-HAP, dissolve and mix them evenly with LAP solution, and the final concentrations of the three are 0.03% (w / w), 23% (w / w) and 3% (w / w) respectively. / w), namely get example 2 cartilage calcification layer sol.

[0063] Subchondral bone layer sol: weigh quercetin, GelMA and Fe-HAP, dissolve and mix them evenly with LAP solution, the final concentrations of the three are 0.03% (w / w), 28% (w / w) and 3% respectively (w / w), promptly obtains...

Embodiment 3

[0065] A method for preparing a gradient osteochondral biomimetic scaffold integrated with diagnosis and treatment, which is basically the same as in Example 1, except that the sol ratio of each layer is different:

[0066] Cartilage lamellar sol: Weigh quercetin and GelMA, dissolve and mix them evenly with LAP solution, the final concentrations of the two are 0.03% (w / w) and 20% (w / w) respectively to obtain cartilage lamellar sol, namely Example 3 cartilage lamellar sol.

[0067] Cartilage calcification layer sol: Weigh quercetin, GelMA and Fe-HAP, dissolve and mix them evenly with LAP solution, and the final concentrations of the three are 0.03% (w / w), 25% (w / w) and 3% (w / w) respectively. / w), namely get example 3 cartilage calcification layer sol.

[0068] Subchondral bone layer sol: Weigh quercetin, GelMA and Fe-HAP, use LAP solution to dissolve and mix evenly, and the final concentrations of the three are 0.03% (w / w), 30% (w / w) and 3% ( w / w), promptly get example 3 subc...

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Abstract

The invention discloses a diagnosis and treatment integrated gradient osteochondral bionic scaffold and a preparation method thereof, which adopts a DLP photocuring 3D printing technology, and the preparation method comprises the following steps: respectively preparing sol of a cartilage layer, a cartilage calcification layer and a subchondral bone layer, and preparing cartilage layer sol by utilizing quercetin, methacrylate esterified gelatin and an LAP solution; preparing cartilage calcification layer sol by utilizing quercetin, methacrylate esterified gelatin, iron-doped hydroxyapatite andan LAP solution; preparing subchondral bone layer sol by utilizing quercetin, methacrylate esterified gelatin, iron-doped hydroxyapatite and an LAP solution. The scaffold prepared by the method has structural gradient and material gradient, has good mechanical properties and biocompatibility, and has good bone induction capability and bone conduction capability, which can induce regeneration of bone tissue defect parts. The gradient scaffold provided by the invention has an MRI diagnosis function, which can be used to accurately evaluate the real-time condition of osteochondral defect repair in vivo.

Description

Technical field [0001] The invention belongs to the technical fields of medical biomaterial preparation and tissue engineering, and in particular relates to a diagnosis and treatment integrated gradient osteochondral bionic scaffold and a preparation method thereof. Background technique [0002] According to statistics, 70% of people over 65 years old suffer from varying degrees of articular cartilage damage. Articular cartilage damage is a common disease caused by trauma, disease, improper exercise, aging, etc., and often manifests as joint pain. , functional impairment, seriously affecting the patient's daily life. Since human articular cartilage is an avascular tissue, and the required nutrients mainly come from synovial fluid, this nutrition is not enough to promote the repair and reconstruction of cartilage defects; and the relative renewal rate of chondrocytes is low. When the defect of articular cartilage is >2 mm, At this time, the defect can hardly be repaired b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/56A61L27/22A61L27/12B33Y70/10B33Y80/00A61F2/82
CPCA61L27/54A61L27/56A61L27/222A61L27/12B33Y70/10B33Y80/00A61F2/82A61L2430/06A61L2430/02A61L2300/412A61L2300/216A61F2240/002
Inventor 陈盛贵李楠花开慧卢秉恒
Owner DONGGUAN UNIV OF TECH
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