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Method for establishing zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration

A technology of embryogenic cells and suspension culture, applied in plant regeneration, botany equipment and methods, horticultural methods, etc.

Active Publication Date: 2014-06-25
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the establishment of embryogenic cell suspension culture lines and regenerated plants on Allium genus

Method used

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  • Method for establishing zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration
  • Method for establishing zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration
  • Method for establishing zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Embryogenic induction of embryogenic callus: Take the young flower stalks of Allium scallion, after surface disinfection treatment, cut the leaves into 1-3 cm long fragments, and inoculate them on the embryogenic induction medium of embryogenic callus, Culture in a dark environment at a temperature of 25-27°C for 12-16 weeks to obtain bright yellow loose and fragile embryogenic callus (see figure 1 ).

[0035] Wherein the embryogenic callus induction medium is based on MS medium as the basic medium, and also contains 0.5 mg / L of TDZ, 1.0 mg / L of 2,4-D, 20-40 g / L of sucrose And 8 grams of agar, the pH value is 5.6~6.0.

[0036] The inventor found in the research that: when the explants were cultured on the induction medium, calluses of different colors would appear, one was white and the other was bright yellow; when the culture medium was different, two kinds of callus appeared ratios vary. If white callus is used, it is not conducive to the establishment of sub...

Embodiment 2

[0043] The difference between this example and Example 1 is that the embryogenic callus induction medium contained in the step (1) contains 1.0 mg / L TDZ and 3.0 mg / L 2,4-D. All the other are identical with embodiment 1. Under this condition, the embryogenic callus induction rate of Allium scallion was 26.7%.

Embodiment 3

[0045] The difference between this example and Example 1 or 2 is that the embryogenic callus induction medium in step (1) contained 1.0 mg / L TDZ and 1.0 mg / L 2,4-D. All the other are identical with embodiment 1 or 2. Under this condition, the embryogenic callus induction rate of Allium scallion was 47.3%.

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Abstract

The invention discloses a method for establishing a zephyranthes candida embryogenic cell suspension culture system and performing plant regeneration. The method is the first one for establishing an embryogenic cell suspension culture system on a zephyranthes candida plant. The highest embryonic callus induction rate can be 45.2 percent; the embryogenic cell suspension culture system is rapid in multiplication, the fresh weight of embryogenic cells can be multiplied by 2.9 times to the highest extent each week, and the highest plant regeneration rate of the embryogenic cells can be 85.3 percent.

Description

technical field [0001] The invention relates to the technical field of plant induced regeneration, in particular to a method for establishing a suspension culture line of embryogenic cells of lotus allium and regenerating plants. Background technique [0002] Onion lotus ( Zephyranthes candida Herb.), also known as onion orchid, jade curtain, and white calamus lotus, is an important perennial bulbous flower of the Amaryllidaceae Allium genus. It can also be used medicinally and is native to America. Onion lotus is resistant to barrenness, easy to survive, has many flowers, and has a long flowering period. It is widely used in urban landscaping, such as flower beds, flower borders, roadsides or under forests, and slopes as ground cover plants. [0003] Tissue culture studies on Allium genus plants began in the 1970s, and Furmanova and Oledzka (1976; 1981) reported earlier that the Allium genus Z. robusta Regenerated plantlets were obtained by in vitro induction of scales. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 于波朱根发孙映波
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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