Tissue engineering meniscus repair sheet and preparation method thereof
A technology of tissue engineering and meniscus, which is applied in the field of tissue engineering medical biomaterials, can solve the problems of inability to promote the fusion of materials and tissues, the lack of nutrition in joint synovial fluid, and the difficulty of firm attachment of cells, so as to achieve firm attachment and short inoculation time. Short, high inoculation rate effect
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example 1
[0031] The seed cells used in Example 1 were human bone marrow mesenchymal stem cells purchased from Beijing Yuhengfeng Technology Co., Ltd.
example 2
[0032] The seed cells used in example 2 are rabbit fibrochondrocytes, and the isolation and culture methods are referred to literature: Pan Xianfeng, Lin Yueqiu, Tang Xun etc. The cultivation and biological characteristics of rabbit meniscus fibrochondrocytes, Chinese Journal of Orthopedic Surgery, October 2000 Expect.
[0033] The ultraviolet laser drilling instrument in Example 1 is Wuhan Chutian Laser Co., Ltd. PSV-6001 type, and its ultraviolet wavelength is 355nm.
[0034] In Example 2, the instrument entrusted to Suzhou Delong Laser Co., Ltd. for drilling is DeepPioneer picosecond laser fine micromachining equipment.
[0035] The 5mL flat-bottomed centrifuge tube used in the example is the LXG-5 model produced by Jiangsu Haimen Bedford Experimental Equipment Company.
Embodiment 1
[0037] Step 1. Cultivation of human bone marrow mesenchymal stem cells: resuscitate purchased first-generation human bone marrow mesenchymal stem cells and inoculate them in human bone marrow mesenchymal stem cell culture medium, and culture them in a cell incubator. The first time after 3 days Change the medium, and then change the medium every other day. After 5 days, the cells reach more than 80% confluence, and then digest with 0.25% trypsin solution to obtain the second-generation human bone marrow mesenchymal stem cells for use; the human bone marrow mesenchymal stem cells cultured The base is α-MEN medium containing 10% fetal bovine serum (FBS);
[0038]Step 2. Preparation of Growth Factor Sustained Release Carrier: Preparation Method of Chitosan Microspheres References: Chen Yu, Liu Zhaoli, Wu Qinghui, etc. Preparation of Micron Chitosan Microspheres. Journal of Dalian University of Technology, Issue 03, 2012 , the particle size of the prepared chitosan microspheres wa...
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