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Tissue engineering meniscus repair sheet and preparation method thereof

A technology of tissue engineering and meniscus, which is applied in the field of tissue engineering medical biomaterials, can solve the problems of inability to promote the fusion of materials and tissues, the lack of nutrition in joint synovial fluid, and the difficulty of firm attachment of cells, so as to achieve firm attachment and short inoculation time. Short, high inoculation rate effect

Active Publication Date: 2014-07-16
西安博鸿生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that the tissue engineered meniscus prepared by articular chondrocytes combined with acellular meniscus scaffold material can be used for meniscus tear repair, but the structure of the acellular meniscus scaffold material is dense, and the cells are difficult to attach firmly. And it is easy to fall off after implanted in the body, and the synovial fluid has less nutrition, which cannot promote the fusion of the material and its own tissue.

Method used

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  • Tissue engineering meniscus repair sheet and preparation method thereof
  • Tissue engineering meniscus repair sheet and preparation method thereof
  • Tissue engineering meniscus repair sheet and preparation method thereof

Examples

Experimental program
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Effect test

example 1

[0031] The seed cells used in Example 1 were human bone marrow mesenchymal stem cells purchased from Beijing Yuhengfeng Technology Co., Ltd.

example 2

[0032] The seed cells used in example 2 are rabbit fibrochondrocytes, and the isolation and culture methods are referred to literature: Pan Xianfeng, Lin Yueqiu, Tang Xun etc. The cultivation and biological characteristics of rabbit meniscus fibrochondrocytes, Chinese Journal of Orthopedic Surgery, October 2000 Expect.

[0033] The ultraviolet laser drilling instrument in Example 1 is Wuhan Chutian Laser Co., Ltd. PSV-6001 type, and its ultraviolet wavelength is 355nm.

[0034] In Example 2, the instrument entrusted to Suzhou Delong Laser Co., Ltd. for drilling is DeepPioneer picosecond laser fine micromachining equipment.

[0035] The 5mL flat-bottomed centrifuge tube used in the example is the LXG-5 model produced by Jiangsu Haimen Bedford Experimental Equipment Company.

Embodiment 1

[0037] Step 1. Cultivation of human bone marrow mesenchymal stem cells: resuscitate purchased first-generation human bone marrow mesenchymal stem cells and inoculate them in human bone marrow mesenchymal stem cell culture medium, and culture them in a cell incubator. The first time after 3 days Change the medium, and then change the medium every other day. After 5 days, the cells reach more than 80% confluence, and then digest with 0.25% trypsin solution to obtain the second-generation human bone marrow mesenchymal stem cells for use; the human bone marrow mesenchymal stem cells cultured The base is α-MEN medium containing 10% fetal bovine serum (FBS);

[0038]Step 2. Preparation of Growth Factor Sustained Release Carrier: Preparation Method of Chitosan Microspheres References: Chen Yu, Liu Zhaoli, Wu Qinghui, etc. Preparation of Micron Chitosan Microspheres. Journal of Dalian University of Technology, Issue 03, 2012 , the particle size of the prepared chitosan microspheres wa...

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Abstract

The invention relates to a tissue engineering meniscus repair sheet and a preparation method thereof. The prepared tissue engineering meniscus repair sheet comprises an acellular meniscal thin sheet, seed cells and growth factor slow-release carriers, wherein blind micro vias are uniformly distributed on two surfaces of the acellular meniscal thin sheet, and the seed cells and the growth factor slow-release carriers are combined on two surfaces of the acellular meniscal thin sheet. The blind micro vias distributed on the acellular meniscal thin sheet increase the area of a support material, and can be used as a storage pool of the seed cells and the growth factor slow-release carriers, so that the seed cells and the growth factor slow-release carriers can be firmly attached and are not easy to fall off. Nutrition can be persistently released through the slow-release carrier by multiple growth factors, the cell amplification efficiency is improved, the cell phenotype is also maintained, cell ageing is inhibited, and fusion of the tissue engineering meniscus repair sheet and self tissue is promoted. The prepared tissue engineering meniscus repair sheet has the structure characteristic similar to the structure characteristic of natural meniscus, and is applicable to repair meniscus injure and induce regeneration of meniscus fibrous cartilage tissue.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, in particular to a tissue engineering meniscus repair sheet with natural structure and function and a preparation method thereof. Background technique [0002] The menisci are two half-moon-shaped fibrocartilages located on the medial and lateral articular surfaces of the tibial plateau. They have the functions of stabilizing the knee joint, transmitting and distributing the load force of the knee joint, and promoting nutrition in the joint. Important organization of functions. Meniscal injuries are mostly caused by torsional external forces, causing joint pain, limited joint mobility, muscle atrophy, and walking inconvenience, which seriously affect the lives of patients. Meniscal injuries are one of the most common sports injuries of the knee. [0003] From the outside to the inside of the meniscus, the outer 10-20% is the red area, which is supplied by the inn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/54A61L27/56A61L27/50A61L27/22A61L27/24A61L27/20
Inventor 张立娟刘影
Owner 西安博鸿生物技术有限公司
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