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Fat extract, fat acellular matrix and preparation method and application of fat acellular matrix

A technology of acellular matrix and extraction liquid, applied in the field of fat acellular matrix and its preparation, and fat extraction liquid, can solve the problems of increasing operation difficulty and biological pollution, and achieve the advantages of shortening processing time, avoiding damage and high biological safety. Effect

Inactive Publication Date: 2020-04-28
鲁峰 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the extract prepared by the conventional above method requires in vitro laboratory culture technology and related equipment, which increases the difficulty of operation and the possibility of biological contamination. Therefore, it is necessary to find a more convenient, economical and safe method to obtain biologically active factors.

Method used

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  • Fat extract, fat acellular matrix and preparation method and application of fat acellular matrix
  • Fat extract, fat acellular matrix and preparation method and application of fat acellular matrix
  • Fat extract, fat acellular matrix and preparation method and application of fat acellular matrix

Examples

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Effect test

Embodiment 1

[0043] Example 1: Preparation of fat extract (Adipose liquid extract, ALE) and fat decellularized matrix M-DAT (Mechanical processing DAT: DAT prepared by physical method)

[0044]1) Wash the obtained granular fat with phosphate buffered saline (PBS), centrifuge at 1000g / min for 3min, discard the upper layer of fat and the bottom layer of liquid after centrifugation, and collect the middle layer of adipose tissue.

[0045] 2) The adipose tissue obtained in step 1) was mixed with an equal volume of PBS, and transferred to a 10 ml screw syringe.

[0046] 3) Connect the fat-containing syringe obtained in step 2) with a new 10ml screw syringe through a 2.4mm caliber Luer connector, push the fat-containing syringe piston, and push the fat into the contralateral syringe, The bolus injection speed is 10ml / s, so that the back-and-forth bolus injection is continued for 1min.

[0047] 4) Centrifuge the bolus product obtained in step 3) at 2000g / min for 5min, discard the upper layer of ...

Embodiment 2

[0055] Example 2: Preparation of fat extract ALE and fat decellularized matrix M-DAT

[0056] 1) Wash the obtained fat particles with buffered saline (PBS), centrifuge at 800g / min for 2min, discard the upper layer of fat and bottom liquid after centrifugation, and collect the middle layer of adipose tissue.

[0057] 2) The adipose tissue obtained in step 1) was mixed with an equal volume of PBS, and transferred to a 10 ml screw syringe.

[0058] 3) Connect the fat-containing syringe obtained in step 2) with a new 10ml screw syringe through a 1.4mm caliber Luer connector, push the fat-containing syringe piston, and push the fat into the contralateral syringe, The bolus injection speed is 8ml / s, so continuous back and forth bolus injection for 0.5min.

[0059] 4) Centrifuge the bolus product obtained in step 3) at 1500g / min for 3 minutes, discard the upper layer of fat, collect the bottom liquid part as ALE and store it at -20°C for later use, and deoil the middle layer after b...

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Abstract

The invention relates to a fat extract, a fat acellular matrix as well as a preparation method and application of the fat acellular matrix. The method comprises the following steps: cleaning and centrifuging fat tissue, mixing middle-layer fat tissue with PBS, and transferring the mixture into an injector (I), wherein the volume ratio of the middle-layer fat tissue to the buffer salt solution is (1 + / -0.2): 1; butting the injector (1) and an injector (2) through a joint, pushing a piston of the injector (1), continuously pushing and injecting back and forth to obtain slurry, and centrifuging to obtain fat extract and middle-layer fat tissue; and carrying out freezing-unfreezing cycle treatment, enzyme treatment and disinfectant treatment on the middle-layer fat tissue to obtain the fat acellular matrix. The invention provides a preparation method of a fat extract and a fat acellular matrix. The method is rapid and efficient, and the fat extract containing growth factors and the fat acellular matrix highly retaining natural extracellular matrix components and structures can be prepared.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a fat extract, a fat decellularized matrix and a preparation method and application thereof. Background technique [0002] Decellularized bioscaffolds composed of natural extracellular matrix (ECM) have the potential to induce regeneration of defective tissue, and have received extensive interest and attention in the field of tissue engineering. Because adipose tissue can be obtained in large quantities through liposuction, a simple method, adipose adiposetissue (DAT) has gradually become a very promising option for soft tissue expansion and repair. [0003] Compared with synthetic scaffolds, natural DAT has the following advantages: (1) DAT retains the natural microstructure and composition of ECM, and can simulate the tissue-specific biochemical and biomechanical properties of the natural cell microenvironment; ( 2) It has no cell components and does not cause immune reject...

Claims

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Application Information

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IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3687A61L27/3691A61L2430/40
Inventor 鲁峰何云帆关静妍
Owner 鲁峰
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