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Cardiac muscle tonifying tablet taking acellular biological membrane as carrier as well as preparation method and application of cardiac muscle tonifying tablet

A biofilm carrier and biofilm technology, applied in prosthetics, medical science, etc., can solve the problems that myocardial patches cannot exert therapeutic effects, cannot provide target cell sheets, and the survival rate of myocardial patches is low, so as to improve decellularization. Efficiency, good biocompatibility, long-term growth effect

Active Publication Date: 2015-03-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, combined with the actual situation of clinical application, it is not difficult to find that the above-mentioned technology still has a treatment bottleneck: in the early stage after the in vivo transplantation, before the myocardial patch has established an effective blood supply connection with the myocardial infarction area, the in vivo environment cannot provide sufficient support to maintain the target cell sheet. The supply of nutrients needed for survival, coupled with the fact that the transplanted myocardial patch products directly face the harsh stress microenvironment including ischemia, inflammation and various pro-apoptotic factors induced by the damaged myocardium, the myocardial patch products after transplantation slice survival rate is low
Therefore, related products in this field all have a common defect that is not easy to overcome: the survival rate after in vivo transplantation is low, so that the transplanted myocardial patch cannot exert the maximum therapeutic effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of bone marrow mesenchymal stem cell myocardial patch with decellularized amniotic membrane as carrier

[0051] 1. The following processes are all aseptic operations, and the reagents used are all sterilized.

[0052] I. Preparation of the bone marrow mesenchymal stem cell myocardial patch with decellularized amniotic membrane as the carrier of the present embodiment

[0053] (1) Decellularization of natural biofilm

[0054] Fresh amniotic membrane (25cm 2 ) was scrubbed repeatedly with sterile normal saline to remove blood clots and serous fluid until clean, placed in a sterile square dish, and used carbonate buffer (0.05 μg / ml penicillin G and 100 μg / ml streptomycin sulfate) mol / L, pH=9.0~9.6) Soak 5 times, 10 minutes each time. Put the amnion into sterile pure water and soak in a 37°C water bath for 60 minutes. After soaking, put it into 10ml sterile phospholipase solution (prepared using 0.05mol / L carbonate buffer solution, pH=8~9; phosphol...

Embodiment 2

[0079] Example 2 Preparation of Cardiac Stem Cell Myocardium Patch with Decellularized Pericardium as Carrier

[0080] 1. The following processes are all aseptic operations, and the reagents used are all sterilized.

[0081] I, prepare the cardiac stem cell myocardial patch of the present embodiment with the decellularized pericardium as the carrier

[0082] (1) Decellularization of natural biofilm

[0083] At room temperature, under routine aseptic operation, the fresh porcine pericardium (25cm 2 ) repeatedly scrubbed with sterile normal saline to remove blood clots and serous fluid until clean, placed in a sterile square plate, and used carbonate buffer (0.05 μg / ml penicillin G, 100 μg / ml streptomycin sulfate) containing antibiotics mol / L, pH=9.0~9.6) Soak twice, 5 minutes each time. Place the pericardium into sterile pure water and soak in a 37°C water bath for 30 minutes. After soaking, put it into 10ml of sterile PBS saline solution (0.01mol / L, pH=6~8) containing 0.3%...

Embodiment 3

[0110] Example 3 Preparation of adipose-derived stem cell myocardial patch with decellularized porcine mesentery as carrier

[0111] 1. The following processes are all aseptic operations, and the reagents used are all sterilized.

[0112] I. Preparation of the adipose-derived stem cell myocardial patch using decellularized porcine mesentery as a carrier of the present embodiment

[0113] (1) Decellularization of natural biofilm

[0114] At room temperature, under routine aseptic operation, the fresh porcine mesentery (25cm 2 ) repeatedly scrubbed with sterile normal saline to remove blood clots and serous fluid until clean, placed in a sterile square plate, and used carbonate buffer (0.05 μg / ml penicillin G, 100 μg / ml streptomycin sulfate) containing antibiotics mol / L, pH=9.0~9.6) Soak 5 times, 10 minutes each time. The porcine mesentery was repeatedly frozen and thawed (frozen at -80°C, thawed at 37°C) three times, 1 hour each time. Put 10 mL of 0.05% (w / v) trypsin-EDTA s...

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Abstract

The invention discloses a cardiac muscle tonifying tablet taking an acellular biological membrane as a carrier as well as a preparation method and application of the cardiac muscle tonifying tablet. The cardiac muscle tonifying tablet taking the acellular biological membrane as the carrier is prepared by the following steps: carrying out decellularizing treatment on a natural biological membrane; then, cross-linking nutrient substances on the acellular biological membrane; planting target cells on the acellular biological membrane cross-linked with the nutrient substances; and cultivating a cell sheet which is preliminarily constructed, so that the cardiac muscle tonifying tablet taking the acellular biological membrane as the carrier is prepared. The cardiac muscle tonifying tablet disclosed by the invention is good in proliferation and differentiation activities and mechanical strength, and is sufficient in nutrition support; the cardiac muscle tonifying tablet is not only a breakthrough in tissue engineering but also suitable for the clinical treatment of myocardial infarction. The cardiac muscle tonifying tablet disclosed by the invention is reliable in principle, good in reproducibility, and suitable for standard production.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a myocardial patch with decellularized biofilm as a carrier, a preparation method and application thereof. Background technique [0002] The current treatment methods for myocardial infarction can only eliminate vascular obstruction and relieve ventricular remodeling, but cannot reverse necrotic myocardium. The use of cell therapy from various sources has attracted increasing attention and is considered to be the most potential treatment method. Among them, using various target cells to prepare myocardial patches and attaching them to the myocardial infarction area provides a more effective therapeutic strategy for the treatment of myocardial infarction. The advantages of this type of product are: ①It can be used to treat a larger area of ​​myocardial infarction; ②The initial survival rate of cells is significantly increased; ③The clinical treatment effect produced by the same n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/50
Inventor 武征林熙张建华蔡冬青秦子夕王颖薇张中夏秦俊文齐绪峰禹艳红
Owner JINAN UNIVERSITY
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