71 results about "Cardiac Stem Cell" patented technology

The invention describes the isolation and methods of use of a non-senescent pool of adult cardiac stem cells. Methods for repairing aged myocardium or damaged myocardium using the isolated non-senescent adult cardiac stem cells are also disclosed. In addition, the invention describes a method for preventing or treating heart failure.

Compositions of isolated adult organ stem cells, including hematopoietic stem cells, cardiac stem cells, and kidney stem cells, are disclosed. In particular, the present invention provides c-kit positive, lineage negative adult stem cells that can be isolated from adult organ tissue. Such stem cells are capable of generating all the cell lineages of the organ tissue from which they were isolated. Methods for repairing damaged organ tissue with the isolated organ stem cells are also disclosed.

The invention describes the isolation and methods of use of a non-senescent pool of adult cardiac stem cells. In particular, a subset of adult cardiac stem cells with superior regenerative capacity is disclosed. Such cells were found to have immortal DNA. Compositions comprising the non-senescent stem cells are also described. In addition, the present invention provides methods for repairing aged myocardium or damaged myocardium using the isolated non-senescent adult cardiac stem cells.

The present application relates to cardiac stem cells and a method of using cardiac stem cells to repair damaged heart tissue. In one embodiment, cardiac stem cells, such as cardiosphere-derived cells and / or cardiospheres, can be seeded, embedded and / or cultured in a biomaterial or matrix made from, for example, a hydrogel, that is subsequently administered to a subject to repair damaged heart tissue.

Described herein are reprogrammed cells, and methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also descried are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs), Cardiac Stem-Like Cells (CSLC), Hematopoietic Stem-Like Cells (HSLC), Pancreatic Progenitor-Like Cells, and Mesendoderm-like Cells. Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas.

The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and / or augment the functionality of such cells for cellular therapies.

Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating cardiomyopathies, for treating ischemic heart diseases and for setting in vitro models to study drugs.

Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating genetic cardiomyopathies, for treating ischemic heart diseases and for setting in vitro models to study drugs.

The present invention provides in vitro methods of expansion and propagation of undifferentiated progenitor cells and more specifically undifferentiated progenitor cells containing Islet1, a marker apparently unique to proliferating cardiac stem cells. Methods are described for isolation of stem cell populations as well as for provoking expansion and propagation of undifferentiated progenitor cells without differentiation, to provide cardiac repair or improve cardiac function, for example.

A method and apparatus for local delivery of growth factors which enhances stem cell regeneration of the heart is disclosed. In one example, a stent containing growth factor within openings in the stent delivers the growth factor into a coronary artery to improve effectiveness of the stem cell transplantation therapy. The stent may also be used to transplant stem cells and deliver other bioactive factors.

A biopsy forceps (1) comprises a flexible pipe (2) which can be remotely controlled. Two handles (6 and 7) which are connected to the near end of the pipe are used for operating a pair of clamps (4 and 5) that are connected to the far end of the pipe. An independent control handle (8) comprises a rotatable knob (16). The knob rotates in the reverse direction to facilitate the bending of the pipe in the second direction. A control knob can be connected to the far end of the pipe through the operating lines (42 and 43) that moves in the cavities (44 and 45) of the pipe in company with the rotation of the knob. The clamps (or other control component) at the far end of the pipe are replaceable (80A and 80B).

The present invention relates to compositions and methods for inducing cardiomyogenesis in mammalian cells, particularly embryonic stem cells in vitro and in vivo.

The present disclosure relates generally to methods for the increased processing of tissue for the generation of cardiac stem cells, wherein the stem cells are suitable for use in cardiac stem cell therapy. In particular, several embodiments relate to the processing of allogeneic donor cardiac tissue for the generation of multiple patient doses of cardiac stem cells.

The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and / or augment the functionality of such cells for cellular therapies.

The invention discloses a method for separating heart stem cells from brown fat and splitting cardioblast. The method comprises the following steps: (1) preparing digestive juice: dissolving callagenase, dispase II and pancreatin in the mass ratio of 1:1:0.5-1 in a serum-free culture medium; (2) digesting the brown fat tissue with a digestion bottle added with glass beads and rotors, and then centrifugally collecting the cells; and (3) cultivating the progenitor heart stem cells and splitting the cardioblast. By adopting the combined digestive enzyme liquid and a special digestion method for cell separation, the separation method remarkable improves the yield of cells and the content of heart stem cells compared with the existing separation method, and remarkably improves the yield of the heart stem cells by verifying the expression rate of CD133 and the capability of the cardioblast. By using the method, the yield of the obtained heart stem cells is high, the operation is simple, the implementability is strong and the manual operation is reduced.

The present invention relates to compositions and methods for inducing cardiomyogenesis in mammalian cells, particularly embryonic stem cells in vitro and in vivo.

Compositions comprising stem cells delivered into infarcted myocardium by endocardial injection, engraft and differentiate into myocytes, endothelial cells, and vascular smooth muscle, and do so without the requirement for survival enhancing modification. These cells engraft whether injected acutely (days) or late (months) after myocardial infarction, and the efficiency of engraftment correlates with the functional recovery of the heart. The stem cells also recruit endogenous cardiac precursor cells, reconstitute myocardial stem cell niches, and enhance endogenous cell differentiation into myocytes.

The present disclosure relates generally to methods for the increased processing of tissue for the generation of cardiac stem cells, wherein the stem cells are suitable for use in cardiac stem cell therapy. In particular, several embodiments relate to the processing of allogeneic donor cardiac tissue for the generation of multiple patient doses of cardiac stem cells.

The invention relates to a biologic patch material and concretely relates to a biologic patch material for cardiac repair. The biologic patch material comprises (i) at least two porous structural layers that are compounded together, wherein the porous structural layer comprises a first structural layer for preventing repairing cell loss and a second structural layer for loading (or accommodating) cardiac repairing cells and the repairing cells are selected from cardiac stem cells, cardiac progenitor (precursor) cells or their composition (derived from embryonic stem cells, induced pluripotent stem cells, heart tissue stem cells, bone marrow and peripheral blood), and (ii) repairing cells loaded on the second structural layer, wherein at least a part of or all the repairing cells are located in gaps of the second structural layer. The biologic patch material can load growing of repairing cells and has very good biocompatibility and mechanical strength.

Disclosed herein are methods, compositions and kits for treating cardiac stem cells to be administered to a subject in need thereof, e.g., with a damaged myocardium. The methods, composition and kits of the invention can be used to treat cardiovascular diseases such as heart failure, myocardial infarction and an age-related cardiomyopathy.

The invention discloses a stem cell preparation for treating ischemic cardiomyopathy and a preparation method of the stem cell preparation. The preparation consists of mesenchymal stem cells, cardiac stem cells and vascular endothelial stem cells, and a solvent is platelet-rich plasma lysate of a patient. The stem cells express a corresponding specific identifier and do not express a corresponding non-specific identifier at the same time. The preparation method comprises the following steps: (1), preparing mesenchymal stem cells between a placenta and an umbilical cord; (2), applying different induction culture systems to induce the mesenchymal stem cells in the step (1) to be cardiac stem cells and vascular endothelial stem cells; (3), preparing the solvent which is the platelet-rich plasma lysate; and (4), uniformly mixing and preparing the stem cell preparation. The stem cell preparation disclosed by the invention is rich in resource, easy to prepare, convenient to collect, and free of pain while being applied; moreover, the stem cell preparation is low in immunological rejection after being transplanted. The stem cell preparation comprises a plurality of stem cells, so that advantages of combined effect of a plurality of cells can be brought into play; the solvent is easily available, and can directly act on a patient; besides, transplantation effect is enhanced.

Disclosed herein are compositions and methods for the improved delivery of cells to a target tissue. In some embodiments, the compositions comprise stem cells, in particular cardiac stem cells, and the target tissue is damaged or diseased cardiac tissue. In several embodiments, the methods, in combination with the compositions, yield enhanced delivery, retention, and / or engraftment of the cells into the target tissue, thereby inducing improved functional recovery.

The invention provides a purpose of salvianic acid A sodium for inducing in vitro cultured cardiac stem cell proliferation. The research finds that the salvianic acid A sodium has the effect for inducing in vitro cultured cardiac stem cell proliferation and provides enough stem cells for clinical treatment. The salvianic acid A sodium can be the salvianic acid A sodium with high purity in the market (the content is greater than 98.5%), and can be prepared by a conventional method. The invention has the advantages that the salvianic acid A sodium-induced cardiac stem cell proliferation can provide enough stem cells for treating myocardial infarction, preventing cardiac arrhythmia after infarction during recovery period and treating the stem cells, the salvianic acid A sodium can reduce theorganism immunity rejection reaction and reduce the mortality of acute myocardial infarction, and the therapeutic effect is definite.

Compositions comprising stem cells delivered into infarcted myocardium by endocardial injection, engraft and differentiate into myocytes, endothelial cells, and vascular smooth muscle, and do so without the requirement for survival enhancing modification. These cells engraft whether injected acutely (days) or late (months) after myocardial infarction, and the efficiency of engraftment correlates with the functional recovery of the heart. The stem cells also recruit endogenous cardiac precursor cells, reconstitute myocardial stem cell niches, and enhance endogenous cell differentiation into myocytes.

The invention provides a freezing solution for cardiac stem cell. The stem cell comprises a DMEM basic medium, fetal bovine serum, vitamin E, trehalose and hydroxyethyl starch. The dose of fetal bovine serum is 0.8-2.0% of the weight of the DMEM basic medium. The density of the vitamin E is 18-25 micro mol / Ls. The density of the trehalose is 0.1-0.3 mol / L. The dose of the hydroxyethyl starch is 10-13% of the weight of the DMEM basic medium. A freezing method using the freezing solution is also provided. The freezing solution has no toxic protective agent such as DMSO, and has utterly good cell recovery rate as high as 94.55% for the freezing of cardiac stem cell.

Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating cardiomyopathies, for treating ischemic heart diseases and for setting in vitro models to study drugs.