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Freezing solution and freezing method for cardiac stem cell

A cryopreservation liquid and stem cell technology, applied in the field of cells, can solve the problems of unsatisfactory effect, unfavorable myocardial stem cell recovery, no myocardial stem cell discovery, etc., and achieve the effect of excellent cell recovery rate

Inactive Publication Date: 2017-02-22
CHENGDU YUYA TECH
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the effect of the existing cryopreservation methods for myocardial stem cells is not ideal, and no cryopreservation solution specifically for myocardial stem cells has been found. The cryopreservation solution used usually contains DMSO and other protective agents that are toxic to cells, which is not conducive to myocardial stem cells. stem cell recovery
This greatly limits the research and application of myocardial stem cells in diseases such as myocardial infarction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Prepare freezing solution:

[0016] Different amounts of fetal calf serum, vitamin E, trehalose and hydroxyethyl starch were added to the DMEM basal medium to prepare five kinds of freezing solutions, and the formulations are shown in Table 1.

[0017] Table 1

[0018] The amount of fetal bovine serum added (% by weight in DMEM) Vitamin E concentration (μM) Trehalose concentration (M) The amount of hydroxyethyl starch added (% by weight in DMEM) Recipe 1 0.80 18.00 0.10 10.00 Recipe 2 2.00 25.00 0.30 13.00 Recipe 3 1.50 20.00 0.15 11.00 Recipe 4 1.50 22.00 0.25 12.00 Recipe 5 1.50 21.00 0.20 11.50

[0019] (2) Dilute the primary cultured myocardial stem cells to a concentration of 0.1×10 6 , 0.5×10 6 , and 1.0×10 6 Each was pre-cooled at 4°C for 10-30 min, frozen at -80°C for 24 hours, and transferred to a liquid nitrogen tank for storage. ,

[0020] (3) Recovery of myocardial stem cells: Transfe...

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Abstract

The invention provides a freezing solution for cardiac stem cell. The stem cell comprises a DMEM basic medium, fetal bovine serum, vitamin E, trehalose and hydroxyethyl starch. The dose of fetal bovine serum is 0.8-2.0% of the weight of the DMEM basic medium. The density of the vitamin E is 18-25 micro mol / Ls. The density of the trehalose is 0.1-0.3 mol / L. The dose of the hydroxyethyl starch is 10-13% of the weight of the DMEM basic medium. A freezing method using the freezing solution is also provided. The freezing solution has no toxic protective agent such as DMSO, and has utterly good cell recovery rate as high as 94.55% for the freezing of cardiac stem cell.

Description

technical field [0001] The invention belongs to the field of cell technology, and in particular relates to a cryopreservation solution and a cryopreservation method for cardiac stem cells. Background technique [0002] Myocardial infarction is one of the major diseases that seriously threaten human health. Once it occurs, it will cause irreversible changes in cardiac function. In the early stage, myocardial cell necrosis and dissolution will occur, and in the later stage, cardiac enlargement will occur. Quality of Life. The current existing treatment methods include: drug therapy, coronary intervention and coronary artery bypass grafting, etc. Although the above treatments can improve the symptoms of patients, they cannot fundamentally solve the problem of regeneration and repair of myocardial cells and blood vessels. [0003] At present, myocardial stem cell transplantation has become a new hot spot in myocardial regeneration therapy, and this technology is expected to bri...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 向红先
Owner CHENGDU YUYA TECH
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