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145 results about "Neutral red stain" patented technology

Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.

Method for removing organic cationic dyes from waste water by magnetic nano adsorption material

The invention relates to preparation of a magnetic nano adsorbent and a method for removing organic cationic dyes from waste water by the same, and belongs to the technical field of wastewater treatment. The magnetic nano adsorbent uses Fe3O4 particles as a carrier, and coated humic acid as an active adsorbing component. The method comprises the following steps: adding mixed solution of ferric chloride and ferrous sulfate to alkaline humic acid solution, reacting under anaerobic, heating and stirring conditions, and obtaining the magnetic nano adsorbent after magnetic separation, washing, drying and grinding. The removal rate of methylene blue and neutral red reaches more than 95% by treating organic cationic dye wastewater with the magnetic nano adsorbent. The magnetic nano adsorbent after magnetic solid-liquid separation is regenerated by methanol-acetic acid solution, a desorption solvent recovers methanol and acetic acid by distillation for recycling use. The magnetic nano adsorbent has the advantages of nanoscale size, high active surface radicals, extremely high adsorption removal rate for cationic dyes, simple process, high efficiency and easy control.
Owner:BEIJING FORESTRY UNIVERSITY

Cell toxicity determination method in cigarette mainstream flue gas

The invention relates to a method for mensurating cytotoxicity of mainstream cigarette smoke. The method is characterized in comprising the following steps: firstly, a cultured cell is directly put to the mainstream cigarette smoke diluted by clean air and is exposed; secondly, the cell is subjected to neutral red staining; according to the situation that the cell is ingested with neutral red, a spectrophotometer of a readable porous culturing plate is utilized to mensurate an absorbance value; and the cell is compared with a contrastive sample exposed in the clean air, thereby judging the toxicity of the mainstream cigarette smoke. The method is based on a testing principle that the cultured cell is directly put to the mainstream cigarette smoke and is exposed; the level for ingesting the neutral red by the cell is in positive proportion to the number of viable cells; when the toxic effect of the mainstream cigarette smoke is larger, the survival cells exposed in the mainstream cigarette smoke is fewer, correspondingly the dye absorption amount is less and the absorbance value is lower. The method can quantitatively evaluate the cytotoxicity of a tested object, has the characteristics of simple operation, high sensitivity and reliable result, and creates a new method for mensurating the cytotoxicity of the mainstream cigarette smoke.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Novel method for measuring iron without mercury

The invention relates to a novel method for measuring iron without mercury, which comprises the following steps of: decomposing a test sample through mixed acid of hydrochloric acid, sulfuric acid and phosphoric acid; taking neutral red and sodium diphenylaminesulfonate as indicators; measuring the iron through a SnCl2-TiCl3-K2Cr2O7 volumetric method; reducing Fe<3+> of phi Fe<3+> / Fe<2+>=+0.76V through tin bichloride with phi Sn<4+> / Sn<2+>=+0.16V; adding neutral red reagent when the solution is flavescent, i.e. the solution still contains a small amount of Fe<3+>; taking the color change of the neutral red as an indicator indicating that Fe<3+> is completely reduced to be Fe<2+> according to the principle that the neutral red is reduced to be colorless after the Fe<3+> is reduced completely, and the neutral red reagent is blue in the Fe<3+> solution; dropping titanium trichloride with phi Ti<4+> / Ti <3+>=+0.1V until blue is disappeared; removing excess titanium trichloride through oxidation by a 5.000g. L<-1> sodium diphenylaminesulfonate-potassium dichromate standard solution method; and finally, calculating ion grade of the test sample.
Owner:JIANGXI UNIV OF SCI & TECH

Fish freshness detection test paper

The invention relates to the technical field of fish detection, and particularly relates to fish freshness detection test paper. Each liter of an indicator solution in a reagent layer contains 0.5-1g of bromophenol red, 0.6-1.1g of bromcresol purple, 0.3-0.5g of bromothymol blue, 0.3-0.8g of neutral red, 0.2-0.6g of phenol red and the balance of a solvent. The fish freshness is detected rapidly with low cost and high flexibility. The detection test paper can be used for detecting the freshness of chilled, refrigerated and frozen fish. The using method is simple; and only less amount of fish body surface mucus is dipped when the test paper is used, and the fish freshness can be obtained by comparing the color instantly developed by the test paper with a standard color chart.
Owner:SHANDONG INST OF COMMERCE & TECH

Color/fluorescence double signal visible rapid nitrite detection method, and applications thereof

The invention belongs to the technical field of food, and more specifically relates to a color/fluorescence double signal visible rapid nitrite detection method, and applications thereof. The color/fluorescence double signal visible rapid nitrite detection method comprises following steps: firstly, a carbon dot-neutral red composite system is prepared, wherein carbon dot synthesis and constructionof the carbon dot-neutral red composite system are carried out; a common filter paper is immersed in the carbon dot-neutral red composite system so as to obtain a carbon dot-neutral red composite system detection test paper; a sodium nitrite standard solution is prepared; a color standard colourimetric card and a fluorescence standard colourimetric card of nitrites are prepared; a software is adopted to extract the RGB values of each test paper, a matrix is constructed, and a multivariate linear regression model is constructed; and the content of nitrites in food to be detected is detected. The carbon dot-neutral red composite system possess color/fluorescence double response capacity on nitrites; detection operation steps are simple; the visible results are clear, visual, and accurate; the detection limit is lower; and it is promising for the color/fluorescence double signal visible rapid nitrite detection method to be used in large-scale food nitrite content detection.
Owner:JIANGSU UNIV

Electrochemical methods for generation of a biological proton motive force and pyridine nucleotide cofactor regeneration

Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing the ratio of the reduced:oxidized forms of NAD(H) or NADP(H). Electrically reduced neutral red is able to serve as the sole source of reducing power for microbial cell growth. Neutral red is also able to promote conversion of chemical energy to electrical energy by facilitating the transfer of electrons from microbial reducing power to a fuel cell cathode.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV

Beta-1,3/1,6-glucan, preparation method therefor, and application thereof in preparing immune enhancement and anti-tumor medicine and functional food

The present invention provide a beta-1,3 / 1,6-glucan, a preparation method therefor, and application thereof in preparing immune enhancement and anti-tumor medicine and functional food. The preparation method comprises: drying and milling antarctic brown algae; after degreasing, water extracting, calcium chloride precipitating and anion exchanging resin purification, obtaining glucan with a beta-1,3-glucose main chain and a beta-1,6-glucose branched chain, wherein a molecular weight thereof is between 5-50 kDa. The beta-glucan prepared by the present invention has an immune enhancement activity, which is indicated by swallow of neutral red by in vitro macrophages and an NO release promoting experiment, and has a tumor growth inhibiting effect and a tumor metastasis inhibiting effect which are indicated by an in vivo animal experiment. According to the present invention, beta-1,3 / 1,6-glucan is prepared by using the antarctic brown algae; is characterized in richness and readily availability of raw material sources, simple preparation process, high product purity, strong bioactivity and easiness for industrialization; and has a prospect of being developed into immune enhancement and anti-tumor medicine.
Owner:MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD

Method for measuring cytotoxicity of condensate of main stream smoke of cigarettes

The invention provides a method for measuring cytotoxicity of condensate of main stream smoke of cigarettes, which is characterized by comprising the following steps: 1) preparation of laboratory reagent, 2) preparation of the condensate of smoke, 3) cells inoculation and culture, 4) contamination of the condensate of smoke, 5) neutral red dying, and 6) result and analysis. The content of the method is as follows: on the basis of the principle of neutral red cell toxicity tests, test procedures are improved according to the cytotoxicity characteristics of the condensate of the main stream smoke of cigarettes, and a method for measuring cytotoxicity of condensate of main stream smoke of cigarettes with low hazard is established. In the invention, the step for fixing formaldehyde fixing solution is reduced to cause the test procedures to be simpler, and simultaneously, the condensate of the main stream smoke of cigarettes is a complex mixture system, has the toxic characteristics of low toxicity and the like and improves the diluted culture solution of samples, namely that the concentration of fetal calf serum is regulated from 10% to 5%, therefore, the test result is more accurate and reliable. The method has the advantages of simpler operation, higher sensitivity and more reliable results.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Methods of Transferring Energies to Water, Alcohols and Minerals

InactiveUS20130138183A1Encouraging resultEnhance ACE pathwayMilk preparationBiocideNeutral red stainHumic acid
The use of humic acids, zeolites, magnesium oxide beads and other mineral-containing materials in the activation of ethanol, alcoholic beverages and water is disclosed. Consumption of the energized liquids can have therapeutic benefits. The activated ethanol can be further used with neutral red dye and ultraviolet (UV) light illumination to indirectly enhance the environmental energy absorption properties of other liquids, including drinking water and of mineral containing materials. Mineral activated ethanol can similarly be used with neutral red dye and UV illumination to enhance the alternative cellular energy (ACE) pathway of an individual.
Owner:MARTIN WILLIAM JOHN

Preparation method and application of ratio type fluorescent carbon dot for detecting sertraline and glutathione

The invention discloses a preparation method and application of a ratio type fluorescent carbon dot for detecting sertraline and glutathione, belongs to the field of fluorescent carbon dots, and aimsto provide a preparation method and application of a ratio type fluorescent carbon dot with a specific recognition effect on sertraline and glutathione. The method comprises following steps: dissolving neutral red and polyethyleneimine in secondary water, carrying out ultrasonic treatment to obtain a uniform mixed solution, transferring the uniform mixed solution into a hydrothermal reaction kettle, reacting for 4-8 hours at the temperature of 150-200 DEG C, standing and cooling the reaction product to room temperature after the reaction is stopped, centrifuging the liquid to remove insolublesubstances, collecting a supernatant, and carrying out dialysis treatment in a glass container for at least three days by virtue of a dialysis bag being 500-1000 Da, so as to obtain a pure carbon dotaqueous solution; and performing freeze drying to obtain the orange red fluorescence emission carbon dots. The prepared ratio type fluorescent carbon dot has a specific recognition effect on sertraline and glutathione, wherein the fluorescence of the carbon dot shows orange-green-orange reversible transformation, and the ratio type fluorescent carbon dot is used for detecting sertraline and glutathione and is good in selectivity and high in sensitivity.
Owner:SHANXI UNIV

Ratio type fluorescent carbon dot for Ag<+> and GSH detection and preparation method thereof

The invention provides a ratio type fluorescent carbon dot for Ag<+> and GSH detection and a preparation method thereof. The preparation method of the fluorescent carbon dot includes (1), weighing a certain mass of neutral red, dissolving the neutral red in secondary water, adding a small quantity of triethylamine in the solution, performing ultrasonic treatment to obtain uniformly mixed solution,wherein the mass ratio of neutral red, secondary water and triethylamine is (1-5):10000:(50-150); (2), transferring the mixed solution into a hydrothermal reactor, reacting for 8-10 hours at the temperature of 200-220 DEG C, standing after finishing the reaction, cooling to the room temperature, centrifuging to remove undissolved substance and extracting supernate, subjecting the supernate to dialysis through a 500-1000 Da dialysis bag in a glass container for at least three days, so as to obtain purified carbon dot aqueous solution; (3), freezing to dry the carbon dot aqueous solution to obtain carbon dot with orange-red fluorescence emission. The ratio type fluorescent carbon dot prepared by the method has single identification effect to Ag<+> and GSH, and its fluorescence color can bein reversible conversion between green and orange red; when used for Ag<+> and GSH detection, the carbon dot has good selectivity and high flexibility.
Owner:SHANXI UNIV
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