The invention discloses a test method for
basophilic granulocyte activation and
degranulation. The method comprises: diluting a to-be-tested
allergen to prepare a standard to-be-tested
allergen, setting a
positive control and a
negative control, wherein all to-be-detected samples are heparinized
peripheral blood of a patient and need to be sealed and incubated, then adding a
buffer solution, centrifuging, adding a fluorescent
dyeing mixed solution, lightly mixing until precipitate is suspended, incubating in a dark place, adding an
erythrocyte lysate, uniformly mixing in a vortex manner, incubating in a dark place, diluting, centrifuging, re-suspending the precipitate, adding a
buffer solution after filtering, and detecting by adopting a flow cytometer to obtain an activation and
degranulation ratio. According to the method, the
basophilic granulocyte is not required to be separated and purified, the new specific
basophilic granulocyte marker combination is selected, the use amount ofthe detected sample is small, the
repeatability is good, the error is small, the activation and
degranulation degrees of the basophilic
granulocyte are effectively distinguished, and the detection andthe
technical support are well provided for the clinical
differential diagnosis and the scientific research project of related diseases.