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Basophilia granulocyte analytical reagent and measuring method

A technology for analyzing basophils and reagents, which is applied in the field of cell analysis reagents, can solve the problems of high price, many measured parameters, complex instrument structure, etc., and achieve the effect of reducing dosage, not easy to separate out, and simple reagent components

Active Publication Date: 2009-05-06
BEIJING SHEN MINDRAY MEDICAL ELECTRONICS TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method measures many parameters, requires multiple special measuring devices, and the instrument structure is complex and expensive.

Method used

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  • Basophilia granulocyte analytical reagent and measuring method
  • Basophilia granulocyte analytical reagent and measuring method
  • Basophilia granulocyte analytical reagent and measuring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Prepare basophil assay reagents as follows:

[0056] NP40 800mg / L

[0057] Brij 35 2g / L

[0058] Citric acid 10mM

[0059] h 2 O to 1L

[0060] The pH value of the above reagent is about 2.8. Take 1 mL of the above basophil analysis reagent and mix with 20 μL blood sample, and incubate at 40°C for 10 seconds. Then, the forward and side scattered light signals of the cells were measured on the Mindray BC-5500 hematology analyzer, and the generated scatter diagram was as follows figure 1 . As shown, basophils can be distinguished from other white blood cells using forward and side scatter light signals.

[0061] Another 155 fresh clinical blood samples were taken, and the above-mentioned basophil analysis reagent was used to measure basophils (%) on Mindray BC-5500 blood cell analyzer according to the above method, and the results were compared with the same blood The specimens were analyzed according to the measured values ​​on the sysmex XE-2100 hematology analy...

Embodiment 2

[0070] Prepare basophil assay reagents as follows:

[0071] NP40 800mg / L

[0072] Brij 56 1g / L

[0073] Glycine 20mM

[0074] HCl Amount to adjust pH to 2.5

[0075] h 2 O to 1L

[0076] Using 1 mL of the above-mentioned basophil assay reagent, add 20 μL of blood sample. After incubating at 40°C for 40 seconds, the forward and side scattered light signals of the cells were measured on the Mindray BC-5500 hematology analyzer to generate Figure 4 scatterplot of . As shown, basophils can be classified and counted by forward and side scatter light signals.

Embodiment 3

[0078] Prepare basophil assay reagents as follows:

[0079] Triton X-100 400mg / L

[0080] Brij 35 500mg / L

[0081] Acetic acid 250mM

[0082] h 2 O to 1L

[0083] The pH value of the above reagent is about 2.8. Use 1 mL of the above basophil analysis reagent, mix with 20 μL blood sample, and incubate at 40°C for 10 seconds. Then, the forward and side scattered light signals of the cells were measured on the Mindray BC-5500 hematology analyzer, and the scattergram results were as follows Figure 5 . As shown, basophils can be distinguished from other white blood cells using forward and side scatter light signals.

[0084] Implementation column 4

[0085] Prepare basophil assay reagents as follows:

[0086] Triton X-100 400mg / L

[0087] Brij 56 1g / L

[0088] Potassium hydrogen phthalate 20mM

[0089] HCl Amount to adjust pH to 2.5

[0090] h 2 O to 1L

[0091] Using 1 mL of the above-mentioned basophil assay reagent, add 20 μL of blood sample. After incubating at...

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PUM

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Abstract

The invention discloses an analytical reagent of basophils, which comprises at least one alkyl phenol polyoxyethylene ether, at least one alkyl alcohol polyoxyethylene ether and a buffering agent for adjusting a pH (pondus Hydrogenii) value. The invention also discloses a method of adopting the reagent to determine the basophils. The reagent of the invention can crack red blood cells and platelets, damage cell membranes of other white blood cells except the basophils, and keep the structure integrity of the basophils so as to sort and count the basophils through optical signals determining the sizes and the internal structures of the cells.

Description

technical field [0001] The invention relates to a cell analysis reagent and a cell analysis method, in particular to a reagent for analyzing basophils and a cell analysis method for measuring basophils. Background technique [0002] In a normal human body, the number of basophils is small, accounting for only 0-1% of the total number of white blood cells. However, in diseases such as heavy metal poisoning, Hodgkin's disease, and chronic myeloid leukemia, the number of basophils will increase significantly, so the count of basophils is of great significance for the diagnosis of these diseases. [0003] In the past, some chemical dyes were often used clinically to stain and count basophils, including Wright-Giemsa composite staining, toluidine blue staining, and neutral red staining. Although these methods are simple, intuitive, and effective, they are not suitable for the detection of large-scale clinical specimens because they can only provide the percentage count of basoph...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N33/50
Inventor 匡玉吉徐兵张宝华
Owner BEIJING SHEN MINDRAY MEDICAL ELECTRONICS TECH RES INST
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