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Agents for nucleated red blood cell, basophilic granulocyte and other white blood cell classification

A technology for classifying basophils and white blood cells, which is applied to the reagents for classifying basophils and other white blood cells, and the field of nucleated red blood cells.

Pending Publication Date: 2022-04-12
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The interference of nucleated red blood cells will make the total count of other white blood cells in samples containing nucleated red blood cells biased

Method used

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  • Agents for nucleated red blood cell, basophilic granulocyte and other white blood cell classification
  • Agents for nucleated red blood cell, basophilic granulocyte and other white blood cell classification
  • Agents for nucleated red blood cell, basophilic granulocyte and other white blood cell classification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The components of the first reagent are shown in Table 1, and the volume of each component in Table 1 is adjusted to 1000 mL with pure water.

[0054] Table 1

[0055] Reagent name concentration tris 3.6g / L HCl 8.1g / L Tween 80 0.8g / L tetradecyl sultaine 1.1g / L Cetyltrimethylammonium Chloride 1.6g / L Isothiazolinone 0.04g / L

[0056] The components of the second reagent are shown in Table 2, and each component in Table 2 is adjusted to 100 g with ethylene glycol.

[0057] Table 2

[0058] Reagent name concentration polymethine 0.003g Isopropanol 4.0g

Embodiment 2

[0060] Get 10 parts of blood samples from different human bodies, take the XN1000 hematology analyzer supporting reagent (hereinafter referred to as supporting reagent) produced by Japan Sysmex Co., Ltd., the first reagent and the second reagent (hereinafter referred to as self-made reagent 2) prepared in Example 1 respectively 10 blood samples were processed and filtered, and the nucleated red blood cells, basophils and other white blood cells were classified and counted on the XN1000 hemacytometer produced by Sysmex Co., Ltd., Japan. The experimental results are shown in Table 3.

[0061] table 3

[0062]

[0063] The data in the above Table 3 show that the correlation coefficient of self-made reagent 1 and supporting reagents for the classification and counting results of nucleated red blood cells, basophils and other white blood cells in 10 different human blood samples is >0.95, indicating that self-made reagent 1 and The test results of the supporting reagents are hig...

Embodiment 3

[0065] The components of the first reagent are shown in Table 4, and the volume of each component in Table 1 is adjusted to 1000 mL with pure water.

[0066] Table 4

[0067]

[0068]

[0069] The components of the second reagent are shown in Table 5, and each component in Table 5 is adjusted to 100 g with ethylene glycol.

[0070] table 5

[0071] Reagent name concentration polymethine 0.003g Isopropanol 4.0g

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Abstract

The invention relates to the technical field of in-vitro diagnosis, and particularly discloses a reagent and a kit for classifying nucleated red blood cells, basophilic granulocytes and other white blood cells and application of the reagent and the kit. The reagent for classifying nucleated red blood cells, basophilic granulocytes and other white blood cells comprises a first reagent and a second reagent, the first reagent comprises a buffer agent, organic acid and a surfactant; the second reagent comprises a fluorescent dye and an organic solvent for dissolving the fluorescent dye. By means of the mode, nucleated red blood cells, basophilic granulocytes and other white blood cells can be distinguished, and then the nucleated red blood cells, the basophilic granulocytes and the other white blood cells are accurately counted.

Description

technical field [0001] The present application relates to the technical field of in vitro diagnosis, in particular to a reagent, kit and application for classifying nucleated red blood cells, basophils and other white blood cells. Background technique [0002] In the prior art, the WBC / BASO channel of most of the five-differential blood cell detection equipment will process other white blood cells except basophils, and combine the laser triangular scattering and flow cytometry to calculate the total number of WBC and BASO Counting is performed. Since mature red blood cells do not have nuclei, after the mature red blood cells are lysed, the number of particles obtained by counting is the total number of white blood cells. However, when nucleated red blood cells interfere, the total number of other white blood cells in the sample containing nucleated red blood cells will be deviated. Contents of the invention [0003] Based on this, the present application provides a reagen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/04G01N15/14G01N21/64G01N1/30
Inventor 郑剑通
Owner SHENZHEN DYMIND BIOTECH
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