A kind of white blood cell classification hemolysis agent and its kit
A white blood cell classification and kit technology, which is applied in the field of blood cell analysis, can solve the problems of increasing the complexity of equipment and testing costs, and cannot directly analyze basophils, and achieves the effect of fast and accurate analysis methods and inhibition of continuous damage.
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Embodiment 1
[0028] A kind of hemolytic agent, it is characterized in that, described hemolytic agent comprises nonionic surfactant, anionic surfactant, organic acid and preservative, described nonionic surfactant concentration 12.1g / L, anionic surfactant concentration are 2.0g / L, organic acid concentration 1.0ml / L and preservative concentration 0.5g / L.
[0029] The nonionic surfactant is polyoxyethylene laurylphenol ether, the anionic surfactant is sodium dodecylsulfonate, and the mixture of the organic acid formic acid and acetic acid. The preservative is sodium azide. The pH of the hemolytic agent is 2.0. A white blood cell classification kit, said kit comprising:
[0030] a. The hemolytic agent mentioned above;
[0031] b. Diluent, the composition includes: chloride salt, sodium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and preservatives, also including EDTA-Na 2 ;
[0032] c. Inhibitors, comprising: alkali metal salts, salts containing carbonate or bicarbo...
Embodiment 2
[0040] A kind of hemolytic agent, described hemolytic agent comprises nonionic surfactant, anionic surfactant, organic acid and preservative, described nonionic surfactant concentration 6.2g / L, anionic surfactant concentration are 1.2g / L , The organic acid concentration is 1.3ml / L and the preservative concentration is 0.3g / L.
[0041] The nonionic surfactant is saponin, the anionic surfactant is sodium dodecylbenzenesulfonate, and the organic acid is formic acid. The preservative is Proclin300. The pH of the hemolytic agent is 4.0.
[0042] A white blood cell classification kit, said kit comprising:
[0043] a. The hemolytic agent mentioned above;
[0044] b. Diluent, the composition includes: chloride salt, sodium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and preservatives, also including EDTA-Na 2 ;
[0045] c. Inhibitors, comprising: alkali metal salts, salts containing carbonate or bicarbonate ions, and preservatives.
[0046] The preservative i...
Embodiment 3
[0048] On the XFA9500 automatic five-classification blood cell analyzer produced by Nanjing Pulang Medical Equipment Co., Ltd., using the kit of Example 1 of the present invention, the detection results of the same blood measurement 20 times are shown in Table 1:
[0049] Table 1
[0050] Measurement times
WBC
RBC
HGB
HCT
MCV
PLT
LYM%
MON%
NEU%
EOS%
BSA%
1
8.51
4.55
151
47.3
95.6
156
24.5
7.8
63.3
3.0
1.4
2
8.60
4.51
151
47.6
95.5
155
24.7
7.5
63.4
3.0
1.4
3
8.66
4.50
152
47.5
95.7
154
24.9
7.3
63.3
2.9
1.6
4
8.59
4.49
151
47.8
95.4
154
24.2
7.4
64.5
2.4
1.5
5
8.4
4.46
153
47.9
95.1
153
23.9
7.5
65.0
2.9
0.7
6
8.57
4.52
150
47.5
95.6
158
23.6...
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