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Preparation and application of hemolytic agent

A hemolyzing agent and completely dissolving technology, applied in the fields of chemistry and medical testing, can solve the problems of poor numerical precision of the five classifications of white blood cells, waste liquid polluting the environment, and human environmental poisoning, etc., to facilitate subsequent operations, good lysis ability, and reduce operation time. Effect

Active Publication Date: 2018-05-01
河南新大阳生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this hemolytic agent often contains cyanide, which is toxic to the human body and the environment, and the discharged waste liquid seriously pollutes the environment; some hemolytic agents do not contain cyanide, but the accuracy of the white blood cell classification is poor; Works well, but is expensive

Method used

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  • Preparation and application of hemolytic agent
  • Preparation and application of hemolytic agent
  • Preparation and application of hemolytic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]

[0027] The sum of all the above components equals 100.

[0028] Preparation method:

[0029] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.

[0030] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.

[0031] C: Pour B into A, adjust the pH to 7.35 with trifluoromethanesulfonic acid, and filter through a 0.45 μm membrane filter. Measured conductivity = 12.11Sm / cm;

Embodiment 2

[0033]

[0034] The sum of all the above components equals 100.

[0035] Preparation method:

[0036] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.

[0037] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.

[0038] C: Pour B into A, adjust the pH to 7.40 with trifluoromethanesulfonic acid, and filter with a 0.45 μm membrane filter. Measured conductivity = 12.21Sm / cm;

Embodiment 3

[0040]

[0041] The sum of all the above components equals 100.

[0042] Preparation method:

[0043] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.

[0044] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.

[0045] C: Pour B into A, adjust the pH to 7.38 with trifluoromethanesulfonic acid, and filter with a 0.45 μm membrane filter. Measured conductivity = 12.09Sm / cm;

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Abstract

The invention provides a hemolytic agent applied to differential counting of white blood cells. The hemolytic agent contains a cationic surfactant capable of effectively dissolving red blood cells; the hemolytic agent contains a nonionic surfactant; the nonionic surfactant can cooperate with the cationic surfactant to crack the red blood cells, can rapidly break red blood cell membranes and can completely dissolve the red blood cells and enable the volume of the red blood cell fragments to be reduced so as to cause no interference on the analysis of the white blood cells; trifluoromethanesulfonic acid is added, so that the ductility of the membranes can be reduced, and leakage and hemolysis of the red blood cells are caused; the stability and the dispersibility of the white blood cells inblood samples can be improved; meanwhile, the hemolysis effects of the surfactants can be improved; the surfactants supplement each other, so that the differential counting number of the white blood cells is more accurate; a phosphate buffer solution and disodium edetate dehydrate are added, so that basophilic granulocytes and other white blood cells can be accurately distinguished; sodium chloride is added, so that the integrity of the state of the white blood cells in an isosmotic solution is retained. The hemolytic agent is non-toxic and environmentally friendly, and is excellent in hemolytic effect, high in dispersibility of the blood samples after hemolysis, convenient in subsequent operations, free of damage to instruments and free of harmless treatment on waste liquid.

Description

[0001] The invention relates to the technical field of chemical and medical testing, in particular to the preparation and application of a hemolytic agent. Background technique [0002] Blood tests can detect many diseases. [0003] Blood consists of plasma and blood cells. [0004] Plasma is equivalent to the intercellular substance of connective tissue. It is a light yellow translucent liquid. In addition to a large amount of water, it also contains inorganic salts, fibrinogen, albumin, globulin, enzymes, hormones, various nutrients, and metabolites. etc. These substances have no definite form, but have important physiological functions. [0005] Blood cells, also known as "blood cells", are cells that exist in the blood and can travel throughout the body with the flow of blood. There are three types of blood cells: red blood cells, white blood cells, and platelets. Red blood cells: mature red blood cells without nucleus and organelles, containing heme, the main function ...

Claims

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Application Information

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IPC IPC(8): G01N15/10G01N15/00
CPCG01N15/00G01N15/10G01N15/1031G01N2015/1006G01N2015/011G01N2015/016G01N2015/1028G01N2015/1024
Inventor 王鸿飞杨永峰翟广玉
Owner 河南新大阳生物技术有限公司
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