Preparation and application of hemolytic agent
A hemolyzing agent and completely dissolving technology, applied in the fields of chemistry and medical testing, can solve the problems of poor numerical precision of the five classifications of white blood cells, waste liquid polluting the environment, and human environmental poisoning, etc., to facilitate subsequent operations, good lysis ability, and reduce operation time. Effect
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Embodiment 1
[0026]
[0027] The sum of all the above components equals 100.
[0028] Preparation method:
[0029] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.
[0030] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.
[0031] C: Pour B into A, adjust the pH to 7.35 with trifluoromethanesulfonic acid, and filter through a 0.45 μm membrane filter. Measured conductivity = 12.11Sm / cm;
Embodiment 2
[0033]
[0034] The sum of all the above components equals 100.
[0035] Preparation method:
[0036] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.
[0037] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.
[0038] C: Pour B into A, adjust the pH to 7.40 with trifluoromethanesulfonic acid, and filter with a 0.45 μm membrane filter. Measured conductivity = 12.21Sm / cm;
Embodiment 3
[0040]
[0041] The sum of all the above components equals 100.
[0042] Preparation method:
[0043] A: Take half of the water and add octadecyl trimethyl ammonium chloride, lauroyl trimethyl ammonium bromide, polyethylene glycol octyl benzyl ether, and stir to dissolve completely.
[0044] B: Take the remaining water and add dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, disodium edetate, stir to dissolve completely.
[0045] C: Pour B into A, adjust the pH to 7.38 with trifluoromethanesulfonic acid, and filter with a 0.45 μm membrane filter. Measured conductivity = 12.09Sm / cm;
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