49 results about "Intercellular substance" patented technology

The present invention relates to medicine, in particular, to the methods of measuring a thermal effect and a local metabolism rate of a live tissue, a water content in the intercellular substance as well as a concentration of biochemical blood components, in particular, blood glucose and pressure in the cardiovascular system.

The invention discloses a felt proofing machine-washing finishing method for wool fabric. Under the effect of ultrasonic waves, the wool fabric is subjected to chlorination pretreatment to realize mild scale stripping treatment, and then the wool fabric is subjected to enzyme treatment to realize controlled scale stripping treatment under effect of the ultrasonic waves, so that the inside and outside uniformity of yarns of which the wool surface is subjected to scale stripping is effectively increased. The chlorination pretreatment is combined with the enzyme treatment, so that the longitudinal scale stripping uniformity of fibers is effectively increased, therefore the felt proofing effect of the wool fabric is achieved, and the machine-washing purpose of wool textiles is realized. Meanwhile, the reaction in the method is carried out in a scale layer, so that the damage on intercellular substances of the inner layers of the fibers is reduced, and the wearing performance and the durability of the wool textiles are increased.

The invention discloses bioprotein sponge and a preparation method thereof. According to the characteristics that internal cells of a human body and an animal body adapt to cell growth environments, the bioprotein is freezed into a dry sponge shape through freeze drying technology by utilizing intercellular substances as controlled-release carrieres of growth factors and acts on the trauma of the human body or the animal body in controlled-release medicament feeding mode. The preparation method of the bioprotein sponge changes the traditional preparation process, adopts S / D virus inactivation, and adds protective agent to reduce probability of virus cross infection of people and livestock simultaneously. The CM cation chromatographic purification process improves purity of bioprotein, promotes stanching and tissue union effect of the bioprotein sponge and reduces clinical side effect. An ultrafiltration process is adopted to replace a dialysis process, so that preparation period is shortened, activity of the bioprotein is protected, and production cost is reduced. A method of heating inactivation virus replaces the method of cobalt60 radiation sterilization, so that the fact that radiation rays bring damage to products is avoided, the activity of the bioprotein is protected, and safe and reliable use of products in clinical application is achieved.

The invention relates to the field of biological medicine and specifically relates to a compound for hormonous dermatitis, a preparation, a preparation method and an application thereof. The compound comprises the following components: rose hydrosol, grape seed extract, aloe vera gel, liquorice extracting solution, curcumin, apigenin, oat-beta-glucan, amidogen glucan, water-soluble azone, polylysine, water soluble vitamin E, buxus chinensis, 1,2-glutaraldehyde, compound growth factor, hydroxypropyl-beta-cyclodextrin, EDTA-2Na, collagen, humectants, auxiliary material and natural preservatives. The components in the compound have mild action to skin and are free from irritation; the components are collocated with each other, can prevent inflammation and anaphylaxis caused by hormonous dermatitis on the basis of supplying moisture preservation and can restrain bacteria to infect the damaged skin. The added compound growth factor can promote metabolism and repairing of cells of corium layer, epidermis and especially cuticle and can promote the forming of intercellular substance, so as to repair the damaged skin and achieve the effects of protection and curing.

The invention discloses a medicinal composition for treating ischemic stroke. The medicinal composition comprises one or more of Radix Astragali, Chinese angelica, Eucommia ulmoides, Panax Notoginseng, radix paeoniae rubra, Ligusticum wallichii, Salvia miltiorrhiza and safflower in Sichuan, and one or more of Gambir Plant, rhizoma gastrodiae, cassia seed, Gentiana macrophylla, lumbricus, scorpion and centipede. The traditional Chinese medicinal composition can improve ischemic damaged brain tissue structure, reduces intercellular substance oedema, substantially reduces the level of TNF-alpha and ICAM-1, has an anti-cerebral ischemia damage effect, has the efficacies of blood stasis removing, tissue regeneration promoting, endogenous wind calming and vein relaxing, can substantially reduce the hemiplegia rate and mortality of thrombus hemiplegia, and also can substantially improve acute blood stasis.

The invention relates to a method for preparing a biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha. The enteromorpha biological organic fertilizer is prepared through the processes of slant culture, seed culture, strain culture, preparation of microbial agents, enteromorpha fermentation and preparation. The Alteromonas colwelliana A321 provided by the invention is an autonomously directional induction screened strain and is preserved in CCTCC (China Center for Type Culture Collection) in Wuhan University, with the preservation number of CCTCC NO: M2012132. Characteristic polyose, proteins and cellulose in the intercellular substance and cell walls of the enteromorpha can be efficiently degraded by the microbial agents so as to fully release nutrient elements in the enteromorpha and increase the fermentation speed of the enteromorpha.

The invention discloses a cellulose skeleton material capable of being industrially produced and applied and a preparation method thereof. The material comprises celluloses and lignin; the content ofthe lignin is 0.8-10%; the cellulose skeleton material is kept in a fixed shape size of a traditional wood skeleton; the length size of the skeleton material is 1-50 cm, the width size is 1-50 cm, andthe thickness size is 0.01-5 cm; and microholes are formed in intercellular substances and cell walls of the skeleton material. The method comprises the procedures of preparation of bleaching liquid,steaming / atomization of the bleaching liquid and rinsing. The method retains the traditional wood skeleton to form the cellulose wood skeleton material. The cellulose skeleton material is large in size, big in thickness and adjustable in lignin content within 0.8-10%; epoxy resins with matched refraction rate are filled to obtain a transparent wood with corresponding size and thickness; the lighttransmittance can reach 90%; the light transmission is excellent; and the heat loss can be reduced.

The invention discloses a Ki67 cell nucleus counting method and system based on pathological immunohistochemistry, and the method comprises the steps: placing a pathological section after Ki67 staining under an optical microscope, and collecting an image of the pathological section after Ki67 staining through visual detection equipment CCD; obtaining a Ki67 negative cell RGB image and a Ki67 positive cell RGB image; carrying out intercellular substance interference filtering on the RGB image to generate a binary image of Ki67 negative cells and a binary image of Ki67 positive cell RGB; carrying out element marking on the binary image, and calculating the number and indexes of connected regions of the binary image; calculating the area of each connected region, taking a standard cell connected region as a reference form, and filtering the connected region with the area smaller than the reference form; segmenting the adherent cells according to the standard that the area of the connectedregion is equal to twice of the reference form; marking Ki67 positive cells and Ki67 negative cells according to the index of the final connected region, and performing counting.

The invention discloses an extraction method for artemisia argyi essential oil. The method comprises the following steps: raw material pretreatment, microwave assisted extraction, compound enzyme assisted extraction, steam distillation extraction, condensation and refining. According to the method, artemisia argyi leaves are placed in distilled water for ultrasonic cleaning, so that impurities such as protein and glucose can be simply, conveniently and effectively removed from surfaces of the artemisia argyi leaves, the generation of the impurities during essential oil extraction is reduced, the aim of improving purity of the essential oil is achieved, and extraction cost increase resulting from traditional solvent cleaning and the influence on the purity of the essential oil caused by solvent residual are avoided. Then, microwave assisted extraction, compound enzyme assisted extraction, steam distillation extraction and molecular distillation are combined, cell wall rupture and localswelling of intercellular substances are achieved by microwave assisted extraction and compound enzyme assisted extraction, and thus, intracellular substances are more easily dissolved out; subsequently, the essential oil is thoroughly refined by steam distillation extraction; finally, refining is carried out by molecular distillation, the separation of lightweight molecules from heavy molecules is achieved by dint of difference among mean free paths of molecular movement of different substances, and thus, the purity of the artemisia argyi essential oil is greatly improved.

The invention relates to a method for purifying edible fungus polysaccharide by using an enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology. The method comprises the following steps: adding enzyme into crushed edible fungi, mixing with water, stirring, and performing enzymolysis for a certain period of time; performing microwave radiation for a period of time, then cooling and performing suction filtration to obtain a polysaccharide extract; concentrating the extract, then adding chitosan to perform flocculation, and filtering to remove deposition after flocculation is finished, thus obtaining a purified polysaccharide solution; and drying to obtain relatively pure edible fungus polysaccharide which is tested to have good vitro antioxidation activity. According to the method provided by the invention, an enzymolysis technology is adopted to perform a certain degree of hydrolysis destruction on cell walls and intercellular substances of edible fungi at first, and then a 'body heating' characteristic of microwave is adopted to promote the dissolution of active ingredients. The method is efficient and energy-saving and can reduce the solvent consumption; and chitosan serving as a natural flocculating agent can well remove protein high polymer impurities, is good in impurity removal effect and low in cost, and cannot influence the product quality.

The invention discloses a method for extracting adipose-derived stem cells, and belongs to the technical field of stem cell extraction. The method for extracting the adipose-derived stem cells comprises the steps of fat acquisition, washing, digestion, cleaning, lysis of red blood cells, termination of lysis, culture, re-inoculation, passage and identification. According to the method, adipose tissue is washed repeatedly with a cell washing liquid to effectively remove fat droplets; a digestive enzyme is utilized to digest collagenous fiber in an intercellular substance to release the cells, so that the damage to the cells is small, and the free cells maintain the activity of the cells in a mesenchymal basal culture medium; subculture is carried out on the cells not attached to walls in asupernatant of a cell culture bottle, thereby increasing the survival rate of the cells, and increasing the multiplication rate of the cells. The method for extracting the adipose-derived stem cells has the advantages that the method is simple and convenient; the enzymolysis of the adipose tissue is thorough, and each component cell is released maximumlly; the survival rate of the obtained adipose-derived stem cells is high.

The invention discloses a method for synergistically extracting and preparing active components in ramulus mori (folium mori) by the aid of solid acid reinforcement thermal reaction, belongs to the technical field of extraction of active components in natural products, and particularly relates to methods for regulating and controlling phosphotungstic heteropoly acid catalysis and hydrothermal reaction. The method has the advantages that efficient pharmacological effects can be realized by the ramulus mori (folium mori), the ramulus mori (folium mori) is already widely applied to the industriesof food, medicines, skincare products and the like, the pharmacological effects realized by the ramulus mori (folium mori) are closely related to the active components in the ramulus mori (folium mori), and accordingly improvement of the extraction efficiency for the active components in the ramulus mori (folium mori) and increase of the recycling value of the active component have research significance and realistic economic values; the shortcomings of existing methods for extracting existing active components in existing ramulus mori (folium mori) can be pertinently overcome by the aid of the method for extracting the active components by means of phosphotungstic heteropoly acid catalysis-hydrothermal reaction regulation and control, plant tissues can be synergistically degraded by phosphotungstic heteropoly acid catalysis and the hydrothermal reaction, hydrogen bonds between celluloses and hemicelluloses and compact structures of cell walls can be destructed, mass transfer barrierbetween the cell walls and intercellular substances can be reduced, the active components can be advantageously sufficiently dissolved out, the method includes few and simple extraction conditions andoperation steps and is little in environmental pollution and low in production energy consumption and cost, and the like.

The invention discloses a method for genetic transformation of a cell. An ultrasonic transformation device is utilized, and the following parameters are set for the ultrasonic transformation device: 1) the ultrasonic transmitting frequency is 0.1 to 1,000 KHz; and 2) the ultrasonic output power is 0.01 to 1,000Watt / cm<2>; and under the conditions, a biomacromolecule to be transformed is transformed into microbial cells, microalgae cells and animal and plant cells. The invention also provides the ultrasonic transformation device for implementing the method. The method and the device can be used for microbial genetic transformation, mass and energy transfer among cells and regular transformation of nucleic acid, protein, polysaccharide and medicament in a bioreactor.

The invention discloses a biological 3d printed active biological membrane of improved AMIC for cartilage repair. The method is characterized in that: sodium alginate, gelatin and hyaluronic acid areused as raw materials to prepare mixed hydrogel, cartilage precursor cells and fibronectin are mixed into the hydrogel, a deposition type biological 3D printing technology is used for constructing theporous hydrogel biological membrane, and chemical crosslinking is achieved through calcium chloride soaking to enhance the mechanical property. The materials used in the invention are all natural materials, are low in immunogenicity, good in biocompatibility and wide in source, have certain mechanical properties and can provide good support for cartilage regeneration. In terms of the preparationmethod, gaps of 300-500 microns can promote cartilage regeneration, meanwhile, an excellent porosity structure and the like can also promote intercellular substance exchange and communication, facilitate adhesion of cytokines or cells is, and facilitate growth and proliferation of the cells in the structure.

The invention discloses a seaweed double-effect facial mask and a preparation method thereof and belongs to the technical field of skin care products. Gloiopeltis furcata, serving as a two-component raw material, is soaked in hydrochloric acid for removal of impurities; on one hand, a nitration reaction of a sodium bicarbonate solution is carried out for extracting sodium alginate in fucoidan contained in cell walls and intercellular substances of seaweed, and on the other hand, ion exchange is carried out to convert insoluble calcium alginate, strontium alginate and the like in seaweed tissueinto sodium alginate to dissolve out; and filtering is carried out for removal of impurities of cellulose and the like, acid coagulation purification is carried out, redissolution of a sodium alginate spinning solution in the preparation process is omitted, and thus, people feels comfortable when using the facial mask on the face. The Gloiopeltis furcata is used as the raw material, after extracting is carried out with ethyl alcohol, nutrient substances such as polysaccharide, mannitol and protein in the Gloiopeltis furcata are obtained, activity regeneration of facial cells can be achieved,and water retention and repairing are promoted. The problem that a current facial mask base fabric has poor fit degree with the face, and consequently essence is liable to lose is solved.

The invention discloses soothing anti-wrinkle essence and a preparation method thereof, the soothing anti-wrinkle essence comprises a thickening agent, a humectant, a chelating agent, an emulsion stabilizer, a solvent, a film-forming agent and an antioxidant, and the functional components comprise acetyl hexapeptide-8, palmitoyl pentapeptide-4, hydroxypropyl tetrahydropyrantriol and tetrahydromethylpyrimidine carboxylic acid. The skin is repaired from two aspects of inhibiting neurotransmitter release and stimulating aged cells, and meanwhile, tetrahydromethylpyrimidinecarboxylic acid and hydroxypropyl tetrahydropyrantriol are used for assisting in activating intercellular substances and transferring chemical samples. And a combined moisturizing component is supplemented, so that the dermis layer is kept in a moist state while being subjected to anti-aging stimulation, and the possibility of skin stress is reduced.

The invention relates to a method for purifying herbaceous plant polysaccharide by adopting an enzyme method extraction integrated flocculation technology. The method comprises the following steps: adding an enzyme in to medicinal material particles obtained by crushing, stirring and mixing with water, and performing enzymolysis for a certain period of time; heating the obtained mixture until refluxing, extracting for a period of time, cooling to room temperature, and then performing reduced pressure suction filtration to obtain an extract containing polysaccharide; and concentrating the extract, adding a chitosan solution while stirring to perform flocculation purification, then centrifuging to obtain a purified polysaccharide solution, and drying to obtain herbaceous plant polysaccharide with relatively high purity. According to the method provided by the invention, a biological enzyme extraction technology is adopted to perform a certain degree of hydrolytic destruction on cell walls and intercellular substances of a herbaceous plant, the extraction efficiency of the method is high, and the activity of polysaccharide can be kept to the maximum extent; and a selected flocculant is a food additive namely chitosan which is approved by FDA, so that protein type high polymer impurities can be well eliminated, the purification effect is good, and the cost is low to ensure that whole production link is economic, environment-friendly and safe.

The invention provides a new application of radix dipsaci saponin X in the preparation of medicines, and particularly relates to an application of the radix dipsaci saponin X in the inhibition of occurrence and development of pulmonary fibrosis by inhibiting epithelial intercellular substance transition and fibroblast proliferation so as to prevent or treat pulmonary fibrosis. In the application, the dosage of the radix dipsaci saponin X is in the range of 50-2000mg, preferably 50-1000mg.

The invention relates to the technical field of stem cells, in particular to a culture medium and application thereof and a method for inducing tendon stem cells to differentiate into bone cells. According to the culture medium and application thereof and the method for inducing the tendon stem cells to differentiate into the bone cells, FBS, vitamin C, dexamethasone, beta- glycerophosphate and BMP-2 are added into the basic culture medium as an induction culture solution for tendon stem cell osteogenic differentiation. The number of tendon stem cells which are differentiated into bone cells is increased and the differentiation time is shortened. An experiment shows that the culture solution is used for inducing the tendon stem cells, and the characteristics of bone cells can appear within21days, the cell morphology is changed and the number of alizarin red staining cells is increased; and after 28 days of induction, the morphology of the cells is changed gradually, the cells are in apolygonal shape, calcified nodules with mineral deposits exist in the intercellular stroma, and alizarin red staining is positive.

The invention specifically relates to a method for preparing Kappaphycus alvarezii protoplast by using extract of internal organs of Siganus Concatenalu, which belongs to the field of biotechnology. The method comprises the following steps: feeding omnivorous tropical Siganus Concatenalu with Kappaphycus alvarezii in advance so as to induce Siganus Concatenalu to produce enzyme digesting carragheenan; then preparing mixed enzyme liquid with the internal organs of Siganus Concatenalu as a raw material; and degrading cell walls and intercellular substances of Kappaphycus alvarezii by using the mixed enzyme liquid so as to obtain free protoplast. The method provided by the invention can realize artificial cultivation of a novel strain of Kappaphycus alvarezii in virtue of traditional protoplast cell fusion and callus induction and differentiation and provide a novel approach and related supporting technology for seedling growing and breeding.

The invention provides a novel application of protocatechualdehyde as a drug and particularly relates to an application of protocatechualdehyde to pulmonary fibrosis prevention or treatment realized by inhibiting epithelial intercellular substance conversion and fibroblast proliferation and further inhibiting the occurrence and development of pulmonary fibrosis. According to the application, the dosage range of protocatechualdehyde is 20-2000mg and preferably 20-1000mg.

The invention discloses a new application of cells matched with a tumor cell developmental stage and an extract of the cells, that is, an application of the cells and the extract in preparation of medicines for inhibiting growth or proliferation of tumor cells. The cells matched with the tumor cell developmental stage comprise cells in all stages of the differentiation process from stem cells to mature cells or the embryonic development process; the extract of the cells comprises cell lysate, secretions, intercellular substances and the like. The functional mechanism is as follows: the tumor cells are induced to realize differentiation and maturation through the cells matched with the tumor cell developmental stage and the extract of the cells, so that growth or proliferation of the tumor cells is inhibited, and the purpose of curing tumor is achieved. The tumor cells are induced to realize differentiation and maturation and prevented from growing indefinitely through the cells matched with the tumor cell developmental stage and / or the extract of the cells, and finally, the tumor is cured.

The invention discloses natural anti-aging essence and a preparation method thereof. The natural anti-aging essence is characterized by being prepared from the following raw materials in parts by weight: 15 to 20 parts of aloe essence, 6 to 8 parts of cucumber essence, 10 to 12 parts of glycerol, 3 to 5 parts of glyceryl stearate, 3 to 5 parts of tocopheryl acetate, 2 to 4 parts of polydimethylsiloxane, 0.6 to 0.8 part of xanthan gum, 4 to 6 parts of humectants, 0.6 to 0.8 part of essential oil and 25 to 30 parts of deionized water. A hydrosol catalyst prepared by the preparation method disclosed by the invention is extremely high in catalysis activity and cis-pinane product selectivity; the aloe essence and the cucumber essence which are extracted by the cooperative effect of the hydrosol catalyst and alpha-pinene achieve a good effect of inhibiting tyrosinase, so that formation of melanin can be inhibited, and the moisture of skin can be retained; due to the synergistic effect of the composition, the microcirculation can be effectively increased, the metabolism is promoted, and the substance exchange between cells is enhanced; and therefore, cells can be healed and updated, the cell growth can be prompted, and the skin cells can be activated. If people use the essence disclosed by the invention for a long time, the skin of people can become moisturized, tight and elastic.

The invention relates to the technical field of stem cell culture, in particular to a culture medium, application thereof and a method for inducing differentiation of tendon stem cells into adipocytes. TGF-beta1, EGF and G-CSF are added to a basal medium so as to obtain an induction culture liquid for differentiation of the tendon stem cells into adipocytes, the number of the tendon stem cells which are differentiated into adipocytes is increased, and the time of differentiation is shortened. It is shown through experiments that the tendon stem cells which are induced by the culture liquid canexhibit the characteristics of adipocytes within 21 days, the cell morphology is changed, and the number of Oil red O stained cells is increased; and after 28 days of induction, the cell morphology has gradual change and a polygonal shape, calcified nodules formed through mineral deposition are contained in intercellular substances, and the staining characteristic of oil red O is positive.

The invention provides a bone cement and a preparation method thereof. The preparation method comprises the following steps of (1) dissolving glucose into water, preparing a glucose solution, stirring for 20 to 40min, and setting the rotation speed to 120 to 240r / min; (2) heating the glucose solution to 30 to 50 DEG C, and adding sodium carboxymethyl cellulose, so as to obtain a curing liquid; (3) blending hydroxyapatite, alpha-tricalcium phosphate, beta-tricalcium phosphate, epsilon-polylysine, coral powder, high chain crosslinked carboxymethyl starch, aianine modified polylactic acid, collagen, sodium polyphosphate and the curing liquid, so as to obtain the bone cement. The bone cement has the advantages that the bone cement can be applied to therapy of bone regeneration, plastic and aesthetic surgery and the like; the bone cement is quickly degraded, the porosity is produced, the cells easily attach and grow, and the intercellular substance is produced, so as to improve the therapy effect.

The invention discloses a bamboo leaf extract preparing method. The bamboo leaf extract preparing method takes fresh bamboo leaves, deionized water and cellulase as the raw materials and comprises drying and smashing the fresh bamboo leaves; adding the deionized water and the cellulase into the smashed and dried bamboo leaves, and then uniformly stirring the mixture; heating the uniformly-stirredmixture at a constant temperature; filtering the mixture to obtain filter liquor, and performing decompressed distillation on the filter liquor to obtain creamy extract; drying the creamy extract to obtain a finished product. According to the bamboo leaf extract preparing method, the cellulase can decompose cellulose between cell walls and intercellular substance to accelerate diffusion of effective constituents in the protoplasts of cells as well as to increase the separating speed and quantity of the effective constituents of the bamboo leaves, thereby increasing the extracting efficiency and rate. The bamboo leaf extract preparing method has the advantage of being high in extracting efficiency and rate.

The invention relates to the field of gene therapy biotechnologies, and in particular relates to a chitosan nanoparticle composite capable of treating vascular injury restenosis and vascular smooth muscle proliferation as well as a preparation method, a drug and application thereof. The technical scheme adopted by the invention aims to provide the chitosan nanoparticle composite comprising small interfering (si) RNA (Ribose Nucleic Acid) aiming at PDGF (Platelet Derived Growth Factor)-B mRNA (messenger RNA) and a chitosan nanoparticle carrier thereof. According to the invention, the si RNA with specific inhibition of the PDGF-B mRNA expression is designed and synthetized on the base of a c-sis gene and the nanometer carrier is prepared by using chitosan as a raw material to ensure that the cell proliferation and the intercellular substance forming are inhibited through the local transfection of the smooth muscle cell or other relevant target cells. The chitosan nanoparticle composite gene of the PDGF-B si RNA is better in transfection effect and capable of inhibiting the occurrence and the development of relevant diseases of smooth muscle proliferation so as to achieve the treatment purpose.

The invention relates to the technical field of counter ion electroosmosis extraction, in particular to a flexible temperature control system which comprises a flexible substrate layer, a heating electrode, a detection electrode, a heating circuit and a control system. The heating electrode and the detection electrode are embedded with each other; the heating circuit raises the temperature of an area to be detected, the detection electrode inputs a signal to the control system through the detection electrode access site, and the heating electrode inputs the signal to the heating circuit through an output signal connection site to control the power of the heating circuit. The flexible temperature control system is used for transdermal extraction of the interstitial fluid based on reverse Iontophoresis, so that the amount of extracted tissue fluid can be increased, and the sensitivity of blood glucose detection can be improved.

The invention relates to a formula of a medicament for treating chronic fatigue syndrome, and a use method; the navel-filling meridian and collateral slow-release tong is mainly administrated at navel; external navel administration is adopted without oral administration of a large amount of the drug; the navel is named as Shenque. The Shenque acupoint is a channel for a fetus to obtain nutrition from a mother body before delivery, is directly connected with the abdominal wall during embryonic development; the drug is easy to pass through the navel, enters intercellular substance and is rapidly distributed in blood; the Shenque acupoint is internally connected with the twelve meridians, five viscera and six entrails, four limbs and hundreds of bones, five sense organs, skin, muscles and tendons, and also is an intersection point of yin and yang; therefore, the Shenque acupoint is the best administration position, and the effect is three times higher than that of oral administration. A pill prepared by blended venenum bufonis, frankincense and borneol is put into the navel for slow release and continuous administration, and chronic fatigue syndrome can be relieved within 72 hours.