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Method and device for genetic transformation of cell

A transformation device and genetic transformation technology, which is applied in the field of ultrasonic transformation devices to realize the above method, can solve the problems that ultrasonic instruments are not sold yet, and achieve high throughput and simple operation

Inactive Publication Date: 2011-01-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It mainly includes temperature control, frequency control, power, and time control of ultrasonic devices, and ultrasonic instruments that fully realize these functions are not yet sold in domestic and foreign markets.

Method used

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  • Method and device for genetic transformation of cell
  • Method and device for genetic transformation of cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] Embodiment (taking plasmid DNA transformation microbial cell as example)

[0062] 1) Microbial culture

[0063] The transformed microorganisms are cultured to the logarithmic phase, (the formulations of the culture medium for different species of microorganisms are different, and for specific microorganisms, corresponding culture mediums need to be selected. This is a known technology and will not be described in detail). Take 1 ml of microbial culture in a small green bottle, or directly culture it in a small green bottle.

[0064] 2) Add plasmid DNA to the vial in step 1, the amount of plasmid used is 5 μg.

[0065] 3) Put the small blue vial containing 5 μg of plasmid and 1 ml of microbial culture into the cavity of the ultrasonic instrument, the liquid level in the cavity is slightly higher than the liquid level of the culture medium in the glass bottle, but not completely submerged glass bottle. If the small green bottle floats on the liquid surface of the inner...

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Abstract

The invention discloses a method for genetic transformation of a cell. An ultrasonic transformation device is utilized, and the following parameters are set for the ultrasonic transformation device: 1) the ultrasonic transmitting frequency is 0.1 to 1,000 KHz; and 2) the ultrasonic output power is 0.01 to 1,000Watt / cm<2>; and under the conditions, a biomacromolecule to be transformed is transformed into microbial cells, microalgae cells and animal and plant cells. The invention also provides the ultrasonic transformation device for implementing the method. The method and the device can be used for microbial genetic transformation, mass and energy transfer among cells and regular transformation of nucleic acid, protein, polysaccharide and medicament in a bioreactor.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and specifically relates to a method for transforming biomacromolecules such as DNA, RNA, polysaccharide, and protein into difficult-to-transform microorganisms, microalgae, and animal and plant cells. [0002] The invention also relates to an ultrasonic conversion device for realizing the above method. Background technique [0003] In the field of life science and biotechnology, the genetic transformation methods widely used in microorganisms, animals and plant cells mainly include: chemical transformation, cell fusion, Agrobacterium infection, gene gun and electroporation transformation. For cells that are difficult to transform: such as microalgae and Gram-positive thermophilic cells, it is often difficult to transform them even by electroporation. The reasons are: the electroporation transformation process needs to be carried out in a low-concentration ion state, the preparation process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12M1/00C12M1/38C12M1/36
CPCC12M35/04
Inventor 宋厚辉黄巍林璐徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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