Method for determination of form of selenium in selenium-rich plant material
A technology of selenium-enriched plants and a determination method, which is applied in the field of determination of selenium form after pretreatment of selenium-enriched plant materials, can solve the problems of unclear form of organic selenium, cumbersome operation steps, poor repeatability, etc., and achieve thorough hydrolysis and use The effect of small quantity and low cost
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Embodiment 1
[0057] Example 1 Determination of Selenium Species in Selenium-enriched Corn Stalks
[0058] The steps of the pretreatment method for the determination of selenium speciation in selenium-enriched corn stalks were optimized. The optimization process and results are as follows:
[0059] Weigh 0.2g selenium-enriched corn stalk sample into a 15mL plastic centrifuge tube, add 5mL extraction buffer Tris-HCl, ultrasonically extract for 20min, first add 40mg of cellulase, 50℃, shake at constant temperature for 1h, then add 0.5mg of proteinase K , 50°C, constant temperature shaking culture for 8 hours, then add 0.5 mg protease XIV, 37°C, constant temperature shaking culture for 8 hours, then at 4°C, rotate at 10000 rpm, centrifuge for 30 minutes, and pass the supernatant through a 0.22 μm filter membrane to prepare Test solution on the machine. Set the liquid chromatography conditions as mobile phase (40mmol / L(NH4)2HPO4, pH 6.0); flow rate 1mL / min; injection volume 100μL; peristaltic ...
Embodiment 2
[0078] Selenium speciation determination in embodiment 2 selenium-enriched wheat seedlings
[0079] Weigh 0.2g sample into a 15mL plastic centrifuge tube, add 5mL extraction buffer Tris-HCl, ultrasonically extract for 30min, add 40mg cellulase, 50°C, shake at constant temperature for 1h, then add 1mg proteinase K, shake at 50°C at constant temperature Cultivate for 12 hours, then add 1 mg of protease XIV, 37°C, shake at constant temperature for 12 hours, then centrifuge at 10,000 rpm for 30 minutes at 4°C, pass the supernatant through a 0.22 μm filter membrane, and measure the content of organic selenium on a machine. Other conditions are the same as in Example 1, and the results of the determination of the sample and the recovery of standard addition are shown in the following table.
[0080] Table 5 Optimization condition screening and effect
[0081]
[0082]It can be seen from Table 5 that in the pretreatment method of selenium-enriched wheat seedling selenium speciati...
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