The invention discloses a method for determining the arsenic, antimony and bismuth content of pure chromium by using atomic fluorescence spectrometry. The method comprises the following steps of: weighing a specimen; preparing a sample solution; preparing a calibration solution; drawing a working curve; measuring the concentrations of arsenic, antimony and bismuth in the sample solution; and calculating measurement results to obtain the arsenic, antimony and bismuth content. The method for determining the arsenic, antimony and bismuth content of the pure chromium is a hydride generation atomic fluorescence method, is high in analysis sensitivity, convenient to operate, short in analysis cycle and high in efficiency, has broad application prospect, and can be used for measuring the impurities arsenic, antimony and bismuth in the pure chromium which is a widely used raw material. Brazing performance can be greatly influenced by low-melting point impurity elements such as the arsenic, the antimony and the bismuth, and the like, and once the content of the elements exceeds a standard, the mechanical performance of materials can be greatly degraded, so that an accurate and reliable method is required by the measurement of the content of the elements. The requirements of scientific researches and production on control over the quality of raw materials are met, the rejection rate of the product is lowered, and development and production cost is saved.