Method for determining form of selenium in selenium-enriched edible fungi
A technology of selenium-enriched edible fungi and a determination method is applied in the field of determination of selenium form after pretreatment of selenium-enriched edible fungi, which can solve the problems of unclear organic selenium form, complicated operation steps, poor repeatability, etc. The effect of improving extraction efficiency and low cost
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Embodiment 1
[0056] Selenium form determination in embodiment 1 selenium-enriched Pleurotus eryngii
[0057] The steps of the pretreatment method for the determination of selenium speciation in selenium-enriched Pleurotus eryngii were optimized. The optimization process and results are as follows:
[0058] Weigh 0.2g selenium-enriched Pleurotus eryngii into a 15mL plastic centrifuge tube, add 10mL deionized water as the extraction solution, ultrasonically extract for 20min, add 0.5mg proteinase K, culture at 50°C for 8h at constant temperature, then add 0.5mg protease XIV, 37 Centrifuge at 10,000 rpm for 30 minutes at 4°C, and then pass through a 0.22 μm filter membrane to prepare the test solution for the machine.
[0059] Set the liquid chromatography conditions as mobile phase (40mmol / L(NH4)2HPO4, pH 6.0); flow rate 1mL / min; injection volume 100μL; peristaltic pump speed 65rpm; lamp current / auxiliary cathode: 100mA, 45mA; photomultiplier tube Negative high voltage: 300V; carrier gas fl...
Embodiment 2
[0076] Example 2 Determination of Selenium Species in Selenium-enriched Ganoderma lucidum
[0077] Weigh 0.2g selenium-enriched Ganoderma lucidum into a 15mL plastic centrifuge tube, add 5mL extraction buffer Tris-HCl, ultrasonically extract for 30min, add 1mg proteinase K, 50°C, shake at constant temperature for 12h, then add 1mg protease XIV, 37°C, constant temperature After 12 hours of shaking culture, at 4°C, at 10,000 rpm for 30 minutes, the supernatant was passed through a 0.22 μm filter membrane, and the content of organic selenium was measured on a machine. Other conditions are the same as in Example 1. The results of screening for optimal extraction conditions, determination of samples, and standard addition recovery are shown in the following table.
[0078] Table 5. Optimization condition screening and effect
[0079]
[0080] It can be seen from Table 5 that deionized water was selected as the extractant in the pretreatment method for selenium speciation determ...
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