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Method for determining selenium form in selenium-rich egg

A method for measuring poultry eggs, which is applied in the field of selenium-enriched poultry egg selenium speciation, can solve the problem of high cost, and achieve the effects of thorough hydrolysis, non-toxic reagents, and simple and easy-to-operate methods

Inactive Publication Date: 2012-06-27
SUZHOU SETEK
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The pretreatment method of selenium speciation determination is also reported abroad, Selenium species in leaves of chicory, dandelion, lamb's lettuce and parsley (Darja Mazej, Vekoslava Stibilj, et al.Food Chemistry, 2008, 107: 75-83.) discloses a A method for separately utilizing protease to hydrolyze a sample and then performing a selenium speciation test, the ratio of the amount of protease to the sample is 1:2 to 1:5, and the cost is very high

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  • Method for determining selenium form in selenium-rich egg
  • Method for determining selenium form in selenium-rich egg
  • Method for determining selenium form in selenium-rich egg

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Determination of Selenium Form in Embodiment 1 Selenium-enriched Egg Yolk

[0058] The steps of the pretreatment method for the determination of selenium speciation in the yolk of selenium-enriched eggs were optimized. The optimization process and results are as follows:

[0059] Weigh 0.2g of selenium-enriched egg yolk sample into a 15mL plastic centrifuge tube, add 5mL of extraction buffer Tris-HCl, ultrasonically extract for 20min, add 40mg of lipase to degrease, then add 10mg of proteinase K, 50℃, shake at constant temperature for 8h, then add 10 mg of protease XIV was cultured at 37°C for 8 hours with constant temperature shaking, then centrifuged at 10,000 rpm for 30 minutes at 4°C, and the supernatant was passed through a 0.22 μm filter membrane to prepare the test solution for the machine.

[0060] Set the liquid chromatography conditions as mobile phase (40mmol / L(NH4)2HPO4, pH 6.0); flow rate 1mL / min; injection volume 100μL; peristaltic pump speed 65rpm; lamp c...

Embodiment 2

[0078] Determination of Selenium Form in Embodiment 2 Selenium-enriched Egg Protein

[0079] Weigh 0.2g selenium-enriched egg protein sample into a 15mL plastic centrifuge tube, add 5mL extraction buffer Tris-HCl, ultrasonically extract for 20min, 20mg proteinase K, 50°C, shake at constant temperature for 12h, then add 20mg protease XIV, 37°C, After incubation at constant temperature for 12 hours with shaking at 4°C at 10,000 revolutions per minute, centrifuge for 30 minutes, pass the supernatant through a 0.22 μm filter membrane, and measure the content of organic selenium on a machine. Other conditions are the same as those in Example 1. The results of screening for optimal extraction conditions, determination of samples, and standard addition recovery are shown in the table below.

[0080] Table 5 Optimization condition screening and effect

[0081]

[0082] It can be seen from Table 5 that in the test steps of the method for the determination of organic selenium in sel...

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Abstract

The invention discloses a method for determining a selenium form in a selenium-rich egg, which is characterized by comprising the following steps: 1) pretreating the selenium-rich egg, using an extraction buffer Tris-HCl for extracting a sample of the selenium-rich egg, adding lipase for extracting if the selected selenium-rich sample is a yolk sample, then successively adding protease K and protease XIV with an effective amount for hydrolysis extraction to obtain a sample solution to be measured; directly and successively adding protease K and protease XIV with an effective amount hydrolysis extraction to obtain the sample solution to be measured if the selected selenium-rich egg sample is a egg white sample; 2) using combination of hydride generation-atomic fluorescence spectroscopy and high performance liquid chromatography for respectively determining a solution with a standard selenium form and the sample solution to be measured. The method of the invention is simple and easy to operate, the repeatability is good, and the used reagent has the advantages of no toxicity and no pollution.

Description

technical field [0001] The invention relates to a method for measuring the form of selenium in selenium-enriched poultry eggs, in particular to a method for determining the form of selenium in selenium-enriched poultry eggs after pretreatment. Background technique [0002] Selenium is one of the essential trace elements for the human body and a component of glutathione peroxidase (GSH-Px), which has the functions of anti-oxidation, anti-cancer, anti-aging, improving human immunity, and protecting cardiovascular and cerebrovascular ( Rotruck J T, Pope A L, Ganther H E, et al Selenium: biochemical role as a component of glutathione peroxidase. Science, 1973, 179, 588-590.). There are two-thirds of selenium-deficient areas in my country, and one-third of them are severely selenium-deficient areas. Selenium deficiency in humans and animals can cause Keshan disease, Kaschin-Beck disease, white muscle disease of animals, etc. In addition, some cancers, endemic thyroid disorders a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 朱元元
Owner SUZHOU SETEK
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