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Method for extracting adipose-derived stem cells

A technology of adipose stem cell and extraction method, which is applied in the field of adipose stem cell extraction, and can solve the problems of low cell adhesion rate and low survival rate

Pending Publication Date: 2019-05-10
福建省海西细胞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to solve the above-mentioned deficiencies in the prior art. The present invention provides a method for extracting adipose stem cells, which can effectively solve the problem of low cell adhesion rate and survival of adipose stem cells due to the existence of fat oil droplets and red blood cells during the extraction process. low rate problem

Method used

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  • Method for extracting adipose-derived stem cells
  • Method for extracting adipose-derived stem cells
  • Method for extracting adipose-derived stem cells

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Embodiment 1

[0034] Implementation time of this embodiment: October 18, 2018;

[0035] Location: Experimental Center of Fujian Haixi Cell Bioengineering Co., Ltd.;

[0036] Volunteer: Volunteer of Fuzhou Victoria Plastic and Cosmetic Hospital;

[0037] A method for extracting fat stem cells, comprising the following steps:

[0038] (1) Fat acquisition: Adipose tissue was aseptically obtained from volunteers aged 18 years without systemic diseases, infectious diseases and venereal diseases;

[0039] (2) Washing: Take 20 mL of aseptically obtained adipose tissue, resuspend the adipose tissue with 1 times the volume of washing solution, let stand for 10 minutes, leave the upper layer of adipose tissue after layering, then add 1 times the volume of washing solution to resuspend, Let stand for 10 minutes, discard the lower layer liquid, repeat this washing 8 times, then centrifuge at 400 rpm for 10 min, discard the lower layer liquid;

[0040] (3) Digestion: Add 1 times the volume of digesti...

Embodiment 2

[0052] Implementation time of this embodiment: November 20, 2018;

[0053] Location: Experimental Center of Fujian Haixi Cell Bioengineering Co., Ltd.;

[0054] Volunteer: Volunteer of Fuzhou Victoria Plastic and Cosmetic Hospital;

[0055] A method for extracting fat stem cells, comprising the following steps:

[0056] (1) Fat acquisition: Adipose tissue was aseptically obtained from volunteers aged 45 years without systemic diseases, infectious diseases and venereal diseases;

[0057] (2) Washing: Take 20 mL of aseptically obtained adipose tissue, resuspend the adipose tissue with 2 times the volume of washing solution, let it stand for 8 minutes, discard the lower liquid, add 2 times the volume of washing solution to resuspend, and let it stand for 8 minutes , discard the liquid in the lower layer, repeat the washing for 5 times, centrifuge at 500 rpm for 8 min, and discard the liquid in the lower layer;

[0058] (3) Digestion: Add 2 times the volume of digestive enzyme ...

Embodiment 3

[0069] Implementation time of this embodiment: December 11, 2018;

[0070] Location: Experimental Center of Fujian Haixi Cell Bioengineering Co., Ltd.;

[0071] Volunteer: Volunteer of Fuzhou Victoria Plastic and Cosmetic Hospital;

[0072] A method for extracting fat stem cells, comprising the following steps:

[0073] (1) Fat acquisition: Adipose tissue was aseptically obtained from volunteers aged 30 years without systemic diseases, infectious diseases and venereal diseases;

[0074] (2) Washing: Take 20 mL of aseptically obtained adipose tissue, resuspend the adipose tissue with 1.5 times the volume of washing solution, let it stand for 5 minutes, discard the lower liquid, add 1.5 times the volume of washing solution to resuspend, and let it stand for 5 minutes , discard the lower layer liquid, repeat the washing for 7 times, then centrifuge at 450 rpm for 9 min, discard the lower layer liquid;

[0075] (3) Digestion: Add 1.5 times the volume of digestive enzyme enzyme ...

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Abstract

The invention discloses a method for extracting adipose-derived stem cells, and belongs to the technical field of stem cell extraction. The method for extracting the adipose-derived stem cells comprises the steps of fat acquisition, washing, digestion, cleaning, lysis of red blood cells, termination of lysis, culture, re-inoculation, passage and identification. According to the method, adipose tissue is washed repeatedly with a cell washing liquid to effectively remove fat droplets; a digestive enzyme is utilized to digest collagenous fiber in an intercellular substance to release the cells, so that the damage to the cells is small, and the free cells maintain the activity of the cells in a mesenchymal basal culture medium; subculture is carried out on the cells not attached to walls in asupernatant of a cell culture bottle, thereby increasing the survival rate of the cells, and increasing the multiplication rate of the cells. The method for extracting the adipose-derived stem cells has the advantages that the method is simple and convenient; the enzymolysis of the adipose tissue is thorough, and each component cell is released maximumlly; the survival rate of the obtained adipose-derived stem cells is high.

Description

technical field [0001] The invention belongs to the technical field of stem cell extraction, and in particular relates to a method for extracting fat stem cells. Background technique [0002] Adipose-derived stem cells (ADSCs) are a kind of mesoderm-derived adult stem cells isolated from adipose tissue that can adhere to the wall, have plasticity, adhesion and multipotential differentiation potential. ADSCs can differentiate into a variety of cell types derived from mesoderm, including skeletal muscle cells, cardiomyocytes, chondrocytes, adipocytes, and osteoblasts. In addition, it can differentiate into neural cells derived from ectoderm and pancreatic cells derived from endoderm. ADSCs may also contribute to the generation of vascular endothelial cells and hematopoietic cells. Because adipose tissue exists in large quantities in the human body, it is easy to obtain materials, and a large amount of stem cells can be obtained from a small amount of tissue. It can prolifera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 吕小婷聂雅婷沈少敏沈博惠
Owner 福建省海西细胞生物工程有限公司
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