30results about How to "Shorten the differentiation time" patented technology

The invention discloses a method for preparing retinal pigment epithelia. According to the method, stem cells are induced by utilizing a liver X receptor (LXR) activating agent to be differentiated into the retinal pigment epithelia. Compared with the prior art, the method for inducing the stem cells to be differentiated into the retinal pigment epithelia by utilizing the LXR receptor activating agent has the advantages that: a feed layer is not used, so that potential risks caused by an animal source feed layer are avoided; and the differentiation efficiency can be improved, and the differentiation time can be shortened.

The invention relates to a method for inducing human embryonic stem cells to be differentiated into retinal pigment epitheliums (RPE) in vitro, and belongs to the biology technical field. The pure RPEs can be obtained through one-time passage by means of induced differentiation. Compared with the prior art, the differentiation time from the human embryonic stem cells into the RPEs is shortened. By the adoption of the method, a new approach and guidance are provided for induced directional differentiation of the human embryonic stem cells into the pigment epitheliums.

The invention relates to the technical field of stem cells, and in particular to a human mesenchymal stem cell osteogenic induction differential medium and a preparation method. The human mesenchymal stem cell osteogenic induction differential medium comprises an alpha-MEM / HG-DMEM medium as well as the following components in concentration: 5-50% by volume of fetal calf serum, 10-100mu M of ascorbic acid, 5-50mM of phosphoglycerol, 50-500nM of dexamethasone, 0.1-10nM of an insulin-like growth factor-1 and an 0.5-50nM of insulin-like growth factor-2. By adopting the medium, human mesenchymal stem cell osteogenic induction differential signal activation is intensified through the insulin-like growth factor-1 and the insulin-like growth factor-2, osteoblast proliferation is promoted, then the human mesenchymal stem cell osteogenic induction differential efficiency and specificity are improved, the differential time is shortened, the induction efficiency is improved, meanwhile the preparation method is simple and convenient and convenient to use, and stable and efficient human mesenchymal stem cell osteogenic induction differential can be achieved.

The invention discloses a method for quickly, directly and directionally inducing differentiation from mouse embryonic stem cells to neuroepithelial cells. The method comprises the steps of adding an induction medium containing dorsomorphin, noggin, SB431542 and CHIR99021 to a bulk clone of the mouse embryonic stem cells subjected to adherent culture, to induce the mouse embryonic stem cells to form rosette neurospheres; then adding a second stage of induction medium containing bFGF and performing suspension culture to form suspended neurospheres; and finally, digesting the neurospheres into single cells, and then performing adherent culture with a third stage of induction medium to form the neuroepithelial cells with long-term self proliferation and update. By adopting the method, the traditional EB method is abandoned, the differentiation time is shortened, and the differentiation efficiency reaches over 95%. The method can be used for quickly inducing NE cells to substitute NSC cells to treat central system injury, and has very high clinical applicability.

The invention discloses a novel culture method for in vitro differentiation from human embryonic stem cells to functional myocardial cells. The novel culture method is different from a conventional typical hanging-drop culture method. According to the novel culture method, an embryoid body is formed by adopting a simplified suspension differentiation culture method, serum-free culture liquid is adopted for improvement, and various inducing and nutrition-enriching substances are added into the culture liquid to increase the differentiation rate of the myocardial cells and prolong the survival time of the myocardial cells; a method for differentiation from embryonic stem cells to myocardial cells is further effectively updated and perfected, the proportion of the myocardial cells differentiated by the embryoid body is greatly increased, and a large quantity of myocardial cell sources with powerful functions can be provided for stem cell clinical transplantation treatment technology, drug screening and the like; the method is simple and feasible to carry out, the culture time is shortened, the functions for in vitro differentiation from the embryonic stem cells to the myocardial cells are improved and include pulsation time as well as autorhythmicity and rhythmicity of myocardial cell beating, and the method is good in repeatability.

The invention relates to the technical field of stem cell culture, particularly a method for inducing differentiation of skeletal muscle stem cells into cardiomyocyte-like cells. The culture solution comprises a base culture solution, FBS (fetal bovine serum), 5-aza and a TGF (transforming growth factor) beta. The culture solution can induce differentiation of skeletal muscle stem cells into cardiomyocyte-like cells, and is capable of enhancing the differentiation rate from skeletal muscle stem cells to cardiomyocyte-like cells and shortening the differentiation time. After induction for four weeks, the differentiation rates of every group of cells are calculated, and the result shows that the differentiation rate of the cells in the experimental group is obviously higher than the control group (p<0.05 or p<0.01).

The invention discloses a tissue culture method of graptopetabum paraguayense, relates to a cuttage method of a plant, and belongs to the field of plant cultivation methods. The method is characterized by comprising the following steps: scissoring simple leaf, branch stem or telome, drying pruning wound, smearing 1000ppm of naphthylacetic acid on the pruning wound; transplanting on a dedicated sand bed. The manufacturing method is as follows: washing and screening normal sand to leave 100-mesh of sand, mixing 80% of sand, 10% of soil and 10% of manure, covering a plastic film, tightly closing the greenhouse for 15-20 days, preparing the sand for later use. The cuttage method has the beneficial effects that the root of the graptopetabum paraguayense in the cuttage method disclosed by the invention has no rot phenomenon, and the survival rate is improved to 95%; a plant growth regulator and micronutrient element relate to the root development are used as main agents, the root differentiation time can be shortened, the problem of the graptopetabum paraguayense cuttage technology in application is solved.

The invention discloses a quick asexual propagation method for Elaeagnus X ebbingei 'Gilt edge'. The method comprises the following steps of: selecting triennial Elaeagnus X ebbingei as female parents, re-trimming the female parents in April of the last year before grafting, trimming for grafting stocks in January, selecting eight-year hybrid Elaeagnus X ebbingei as the stocks, performing grafting by a cleft graft method at the end of February and in early March, selecting thick branches germinated on scions as cuttings in the first ten-day period of July, soaking the lower sections of the cuttings for 10 minutes by using hormone, inserting the cuttings into a slotting machine, and performing management by adopting full exposure spraying. By the method, the problem about quick propagationof Elaeagnus X ebbingei seedlings can be effectively solved.

The invention relates to the field of stem cell cultivation and especially relates to a cell culture fluid, an application thereof and a method for inducing bone cell differentiation of skeletal muscle stem cells. The cell culture fluid provided by the invention comprises a base culture solution, a vitamin C, dexamethasone, beta-sodium glycerophosphate, IGF-1, BMP-2, PRP and FBS. The cell culture fluid provided by the invention can be used as an inducing culture fluid for bone differentiation of skeletal muscle stem cells. The quantity of bone cell differentiation of the skeletal muscle stem cells is increased, and the differentiation time is shortened. A test proves that when the cell culture fluid provided by the invention is used for inducing the skeletal muscle stem cells, the characteristics of bone cells can appear within 7d, the cellular morphology is changed within 14d, and the quantity of cells dyed by alizarin red is increased. After the cells are induced for 21 days, the expression quantity of OPN and Col-I genes in the cells is increased and is (p<0.05) obviously higher than the expression quantity of OPN and Col-I genes in comparison examples.

The invention belongs to the technical field of cell biology and particularly relates to culture and three-line differentiation induction methods of adipose tissue-derived mesenchymal stem cells. Theculture method specifically includes: placing adipose tissue into a centrifugal tube, adding normal saline, pipetting with a pipet, standing, sucking out liquid on the lower layer of the tissue, adding collagenase type I / PBS digestive juice with the concentration being 0.1% to perform digestion, and placing the culture bottle into a culture tank of 37 DEG C and with 5% CO2; taking out cells when the cells grow to 80% and merge, adding TrypLE digestive juice, terminating the digestion when the cells start retracting and rounding, and performing subculture and multiplication culture. The differentiation induction method has the advantages that differentiation time is shortened, the method needs 10 days to differentiate the cells into adipose and bones and needs 14 days to differentiate the cells into cartilage, and differentiation efficiency is increased greatly as compared a common induction method which needs 14 days to differentiate cells into adipose and needs 21 days to differentiate the cells into bones and cartilage.

The invention discloses a culture medium combination and method for inducing pluripotent stem cells to differentiate into mesenchymal stem cells. The medium combination includes a first-stage mesenchymal stem cell induction differentiation medium and a second-stage mesenchymal stem cell support medium, and the mesenchymal stem cell induction differentiation medium includes STEMdiff TM ‑ACF mesenchymal induction medium; the mesenchymal stem cell support medium includes basal medium, serum and additives, the additives include: L-glutamine, L-Ascorbic acid, sodium pyruvate, MEM NEAA. The method is to use the medium combination for culturing, including: using the mesenchymal stem cell differentiation medium to induce and culture the pluripotent stem cells for 3 days; on the 4th day, start to change to the mesenchymal stem cell support medium for culturing until maturity mesenchymal stem cells.

The invention relates to the technical field of stem cell culture, in particular to a culture medium, application thereof and a method for inducing differentiation of tendon stem cells into adipocytes. TGF-beta1, EGF and G-CSF are added to a basal medium so as to obtain an induction culture liquid for differentiation of the tendon stem cells into adipocytes, the number of the tendon stem cells which are differentiated into adipocytes is increased, and the time of differentiation is shortened. It is shown through experiments that the tendon stem cells which are induced by the culture liquid canexhibit the characteristics of adipocytes within 21 days, the cell morphology is changed, and the number of Oil red O stained cells is increased; and after 28 days of induction, the cell morphology has gradual change and a polygonal shape, calcified nodules formed through mineral deposition are contained in intercellular substances, and the staining characteristic of oil red O is positive.

The invention relates to the technical field of stem cell culture and especially relates to a cell culture fluid, an application thereof and a method for inducing periodontal ligament stem cells to differentiate into myocardium-like cells. The cell culture fluid provided by the invention contains a basic culture fluid, FBS, 5-aza and Ang-II. The cell culture fluid provided by the invention can increase the differentiation rate of the periodontal ligament stem cells differentiating into myocardium-like cells and can shorten the differentiating time. A result of calculation for the differentiation rate of each group of cells shows that the differentiation rate of experimental group cells is obviously higher than that of the control group (p is less than 0.01).

The invention relates to a method for differentiating macrophages from hiPS. The method comprises the following steps of: 1) culturing human induced pluripotent stem cells (hiPSCs) into embryoid EB; and (2) differentiating the embryoid EB into macrophages in an induced differentiation way, wherein an initial cell planting mode is that 8000 cells are cultured in each hole of each EB. The invention solves the problems of long time consumption and low yield of differentiation of macrophages by iPS. Macrophage differentiation time is shortened, and the number of differentiated macrophages is increased. Moreover, the steps of trophoblast cells and mononuclear cell sorting are not needed, so that a differentiation step is simplified, unnecessary pollution opportunities are also avoided, and clinical-grade macrophage differentiation is established.

The invention discloses a method for rapidly and directly directionally inducing the differentiation of mouse embryonic stem cells into neuroepithelial cells, by adding an induction medium containing dorsomorphin and noggin, SB431542 and CHIR99021 to the adherent cultured mouse embryonic stem cell pellet clones to induce mouse Embryonic stem cells form rosette-shaped neurospheres; then add the second-stage induction medium containing bFGF to form suspended neurospheres; finally digest the neurospheres into single cells, and then use the third-stage induction medium to form adherent cultures Neuroepithelial cells with longer self-proliferation and renewal. The invention abandons the traditional EB forming method, shortens the differentiation time, and the differentiation efficiency reaches more than 95%. The invention can quickly induce NE cells to replace NSC cells to treat central system damage, and has high clinical applicability.

The invention relates to a BGIos228 gene and application thereof. The BGIos228 gene has a nucleotide sequence shown in SEQ ID NO:1, or has a variant with function of promoting plants to quickly differentiate and produce shoots. The invention also relates to a transgenic plant with the BGIos228 gene or the variant thereof. The invention also relates to application of the BGIos228 gene or the variant thereof in promoting plants to quickly differentiate and produce shoots, and a method for promoting plants to quickly differentiate and produce shoots.

The invention relates to the technical field of cell culture, in particular to a method for inducing mononuclear cells to differentiate into dendritic cells. The method comprises the following steps: adding CD14+ mononuclear cells into a complete culture medium I, conducting culturing for 4-5 days, adding the cultured system into a complete culture medium II, and conducting culturing for 1-2 days to obtain the dendritic cells. By controlling the sorting method of the CD14+ mononuclear cells, the number of the non-CD14+ mononuclear cells in the adhesion cells and the PBMC is effectively reduced, the content of the cells capable of being differentiated into the dendritic cells is increased, and meanwhile higher dendritic cells can be obtained under the conditions of short time and low cell factor content. GM-CSF or interleukin can be used as a cell factor to promote differentiation of dendritic cells, when GM-CSF or interleukin, such as interleukin-4, is purely added, the induction rate of DC cells is low, and when GM-CSF and interleukin-4 act together, the induction rate is high and the differentiation time is shortened under the condition of low final concentration of the cell factor.

The invention relates to a BGIos116 gene and application thereof. The BGIos116 gene has a nucleotide sequence shown in SEQ ID NO:1, or has a variant with function of promoting plants to quickly differentiate and produce shoots. The invention also relates to a transgenic plant with the BGIos116 gene or the variant thereof. The invention also relates to the application of the BGIos116 gene and the variant thereof in promoting plants to quickly differentiate and produce shoots, and a method for promoting plants to quickly differentiate and produce shoots.

The invention discloses a culture medium and a culture method for differentiating mammal pluripotent stem cells into definitive endoderm cells. The culture medium is prepared by adding at least one of ATP and NAC to a basic culture medium. The method comprises the following step: culturing the pluripotent stem cells of the mammals by adopting the culture medium, so that the pluripotent stem cells are differentiated into the typing endoderm cells. According to the method, at least one of ATP and NAC is added into the basic culture medium, so that the differentiation efficiency of the mammal pluripotent stem cells differentiated and cultured into the endoderm cells or / and the dosage of Activin A is reduced, and the cost is reduced; the culture medium provided by the invention is relatively low in cost and stable in performance, and can efficiently differentiate the human pluripotent stem cells into the definitive endoderm cells. A low-cost and high-efficiency research system is provided for in-vitro research on endoderm related development problems. Meanwhile, the application cost of regenerative medicine is also reduced.

The invention discloses a quick asexual propagation method for Elaeagnus X ebbingei 'Gilt edge'. The method comprises the following steps of: selecting triennial Elaeagnus X ebbingei as female parents, re-trimming the female parents in April of the last year before grafting, trimming for grafting stocks in January, selecting eight-year hybrid Elaeagnus X ebbingei as the stocks, performing grafting by a cleft graft method at the end of February and in early March, selecting thick branches germinated on scions as cuttings in the first ten-day period of July, soaking the lower sections of the cuttings for 10 minutes by using hormone, inserting the cuttings into a slotting machine, and performing management by adopting full exposure spraying. By the method, the problem about quick propagation of Elaeagnus X ebbingei seedlings can be effectively solved.

The invention provides a composite nutrition regulator for regulating and controlling a bougainvillea speetabilis period and a regulating and controlling method thereof. It is found for the first time that under drought stress, melatonin can shorten the flower bud differentiation time of the bougainvillea speetabilis, the flowering time of the bougainvillea speetabilis can be remarkably advanced, growth hormones such as ethephon and monopotassium phosphate are matched, the time needed for flowering can be further synergistically shortened, meanwhile, cane sugar can promote synthesis of bougainvillea speetabilis pigment, and the yield of the bougainvillea speetabilis pigment is increased. The components are compounded to obtain the composite nutrition regulator, and the composite nutrition regulator can obviously shorten the flowering time, prolong the flowering time and brighten the color of the bougainvillea speetabilis when being used in the regulation and control of the bougainvillea speetabilis in the flowering period. According to the composite nutrition regulator for regulating and controlling the bougainvillea speetabilis in the flowering period and the regulating and controlling method thereof, the flowering of the bougainvillea speetabilis can be promoted, the flowering time is shortened, the flowering time of the whole flowering period is prolonged, and the ornamental value of the bougainvillea

The invention discloses a preparation method and application of human induced hepatic-specified differentiation stem cell. The method comprises the following steps of inoculating human induced pluripotent stem cells (hiPSC) on a six-hole cell culture dish coated with matrigel, and culturing by using an mTeSRTM1 stem cell culture solution; when the growth and cloning of the stem cells are obvious, adding activin A into the stem cell culture solution to treat the human iPS stem cells to carry out induced differentiation of endoderm so as to obtain endoderm cells; and culturing the endoderm cells in an RPMI + B27 culture medium containing bone morphogenetic protein 4 (BMP-4) and fibroblast growth factor-2 (FGF-2), so that the endoderm cell is directionally differentiated, and the hepatic-specified differentiation stem cell is obtained. According to the stem cell hepatic-specified differentiation transplantation method, the hepatic-specified differentiation time is shortened, the regeneration capacity and maturity of the stem cells in the liver of the alpha-1 antitrypsin deficiency transgenic mice are enhanced, the cost is reduced, and the technology has wide prospects in clinical application.

The invention relates to sequences and functions of two eucalyptus genes (namely a WPGS3 gene and a WPGS4 gene) used for increasing plant biomass, belongs to the technical field of biology. At first, RNA (Ribonucleic Acid) in eucalyptus is extracted and is reversely transcribed to cDNA (complementary DNA), and then is subject to deep sequencing to obtain full-length cDNA sequence library of eucalyptus, the similarity comparison is carried out between the sequence of the cDNA sequence library of eucalyptus and amino acid sequences of arabidopsis AtGRF1 protein and arabidopsis AtEBP1 protein published by NCBI (National Center of Biotechnology Information) to obtain the full-length cDNA sequence of the WPGS3 gene and the full-length cDNA sequence of the WPGS4 gene, and finally, the full-length cDNA sequence of the WPGS3 gene and the full-length cDNA sequence of the WPGS4 gene are led into arabidopsis for overexpression. As a result, the biomass of transgenic plants is remarkably improved, and the functions of the two xylophyta genes in growth of dicotyledon are determined through a plant molecular biotechnology method at first.