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BGIos228 gene and application thereof

A kind of use, technology of transgenic plants, applied in the field of genes, can solve the problems such as research on genes related to rice differentiation and emergence that have not been seen

Active Publication Date: 2012-08-22
深圳华大基因农业控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on genes related to rice differentiation and emergence

Method used

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  • BGIos228 gene and application thereof
  • BGIos228 gene and application thereof
  • BGIos228 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: PCR amplification of BGIos228 gene and construction of pMD18-T+BGIos228 recombinant vector

[0057] PCR amplification

[0058] The genomic DNA (gDNA) of Oryza.rifupongon Yuanjiang was extracted using a plant genomic DNA extraction kit (TIANGEN novel plant genomic DNA extraction kit, catalog number: DP320-02), according to the sequence of the gene in the gDNA , respectively design a pair of PCR-specific amplification primers (upstream primer F1, plus restriction enzyme site BamHI and protection base, downstream primer R1, plus restriction enzyme site Xba I and protection base). Using the above-extracted gDNA of Yuanjiang as a template, high-fidelity Ex Taq (TaKaRa, DRR100B) polymerase was used for PCR amplification. As shown in Table 1.

[0059] Table 1 PCR system for target gene amplification

[0060] Composition Volume (μl)

[0061] Genomic DNA 0.2

[0062] dNTPs (2.5mM) 2

[0063] 10×Ex Buffer (containing magnesium ions) 2.5

[0064] Primer F1 (50μ...

Embodiment 2

[0082] Example 2: Construction of p6 recombinant vector

[0083] 1) PCR amplification of maize Ubiquitin promoter fragment and construction of pMD18-T+Ubi recombinant vector

[0084] Ubi promoter PCR amplification

[0085] Genomic DNA (http: / / www.maizegdb.org / ), according to the sequence of the promoter in maize B73gDNA, a pair of PCR-specific amplification primers (upstream primer F2:GG) were designed at the head and tail respectively. CTGCAG TGCAGCGTGACCCGGTCGT (SEQ ID NO: 4), plus restriction enzyme site Pst I and protective base, downstream primer R2: GG CTGCAG AAGTAACACCAAAC (SEQ ID NO: 5), plus restriction site Pst I and protective base). Using the above-extracted gDNA of maize B73 as a template, high-fidelity Ex Taq (TaKaRa, DRR100B) polymerase was used for PCR amplification. As shown in table 2.

[0086] Table 2 PCR system amplified by Ubi promoter

[0087] Composition Volume (μl)

[0088] Genomic DNA 0.2

[0089] dNTPs (2.5mM) 2

[0090] 10×Ex Buffer (co...

Embodiment 3

[0145] Example 3: Construction of p6+BGIos228 recombinant vector

[0146] The pMD18-T+BGIos228 plasmid was extracted and digested with the restriction enzymes BamHI / XbaI to recover the BGIos228 gene fragment. At the same time, the p6 plasmid was extracted and digested with the corresponding restriction enzymes BamHI / XbaI to recover the large fragment, which will be recovered The products were ligated and then transformed into competent cells DH5α to obtain the recombinant vector p6+BGIos228. The positive clones were screened for PCR detection and confirmed by sequencing.

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Abstract

The invention relates to a BGIos228 gene and application thereof. The BGIos228 gene has a nucleotide sequence shown in SEQ ID NO:1, or has a variant with function of promoting plants to quickly differentiate and produce shoots. The invention also relates to a transgenic plant with the BGIos228 gene or the variant thereof. The invention also relates to application of the BGIos228 gene or the variant thereof in promoting plants to quickly differentiate and produce shoots, and a method for promoting plants to quickly differentiate and produce shoots.

Description

technical field [0001] The present invention relates to a gene, in particular to the BGIos228 gene, and its use in promoting the rapid differentiation and emergence of plants, especially monocotyledonous plants. Background technique [0002] Rice is the most populous and oldest crop in the world. Rice is the staple food for more than 2.5 billion people in the world. In the past half century, the technological progress of rice cropping in the "Green Revolution" has promoted the substantial growth of grain production in Asia and the world, greatly improved the rice production capacity of the major rice-producing countries in Asia, and contributed to the economic growth and social stability of these countries. Alleviating population growth plays an important role in food, resource and environmental pressures. However, due to the limitation of Oryza germplasm resources and the limitations of conventional breeding methods in time and space, further genetic improvement of rice i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/82C12N5/10A01H4/00
Inventor 张耕耘蔡雪梅张丰丰束礼平
Owner 深圳华大基因农业控股有限公司
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