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A kind of rice callus differentiation medium and its preparation method and application

A technology for differentiation medium and callus, which is applied to the field of rice callus differentiation medium and its preparation, can solve the problems of time-consuming, many methods, and clutter in drying treatment and radiation treatment, and achieves the improvement of the differentiation rate and growth of green seedlings. Good, cost-saving effect

Active Publication Date: 2017-07-28
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drying treatment and radiation treatment are time-consuming, and radiation treatment methods also require specific instruments; adding copper, silver and other elements will cause additional heavy metal pollution, etc.
In short, the methods mentioned above are many and messy, and some methods are only effective for some specific varieties, and are not applicable to other varieties.

Method used

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  • A kind of rice callus differentiation medium and its preparation method and application
  • A kind of rice callus differentiation medium and its preparation method and application
  • A kind of rice callus differentiation medium and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A rice callus differentiation medium (abbreviated as MSK1N0.5) is prepared by adding KT, NAA and sorbitol to MS basal medium; The concentrations in the wound tissue differentiation medium were 1 mg / L, 0.5 mg / L and 40 g / L; the rice callus differentiation medium also contained sucrose at a concentration of 30 g / L, and Phytagel plant at a concentration of 3 g / L gel.

Embodiment 2

[0051] A rice callus differentiation medium (abbreviated as MSK1N1), which is prepared by adding KT, NAA and sorbitol to MS basal medium; wherein, the KT, NAA and sorbitol in the rice callus The concentrations in the differentiation medium were 1mg / L, 1mg / L and 40g / L respectively; the rice callus differentiation medium also contained sucrose at a concentration of 30g / L and Phytagel plant gel at a concentration of 3g / L.

Embodiment 3

[0053] A rice callus differentiation medium (abbreviated as MSK1N1.5) is prepared by adding KT, NAA and sorbitol to MS basal medium; The concentrations in the wound tissue differentiation medium were 1 mg / L, 1.5 mg / L and 40 g / L; the rice callus differentiation medium also contained sucrose at a concentration of 30 g / L, and Phytagel plant at a concentration of 3 g / L gel.

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Abstract

The invention relates to a rice callus differentiation culture medium as well as a preparation method and an application thereof. The rice callus differentiation culture medium is prepared by adding KT, NAA (Naphthalene Acetic Acid) and sorbitol into an MS basic culture medium or a J3 basic culture medium, wherein the concentrations of the KT, NAA and sorbitol in the rice callus differentiation culture medium are 1-2mg / L, 0.5-2mg / L and 20-50mg / L respectively. By exploring a differentiation hormone proportion and a temperature condition and adding different carbon sources, one efficient rice callus differentiation culture medium is screened, and the rice callus differentiation rate can be remarkably improved.

Description

technical field [0001] The invention relates to a rice callus differentiation medium and a preparation method and application thereof, belonging to the technical field of rice tissue culture and transgene. Background technique [0002] Plant tissue culture technology was developed in the early 20th century. It refers to the in vitro culture of isolated plant organs, tissues, cells, protoplasts, etc. under sterile conditions to induce callus and adventitious buds. et al., the process of further forming a complete plant (Wang Jialin (2006) Overview of Plant Tissue Culture and Its Application Research. 3:86-89). Tissue culture technology has the advantages of controlling the growth and differentiation of cells by using various artificial culture conditions, which has effectively promoted the interdisciplinary development of plant physiology, pathology, pharmacy, biochemistry, genetics, and crop breeding. It is used in many industries such as agriculture, forestry, horticulture...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001
Inventor 黄培劲安保光陈思兰李亚梅
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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