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Method for fast propagation of test tube arrowhead by tissue culture

A technique of tissue culture and test tubes, applied in horticultural methods, botany equipment and methods, horticulture, etc., to achieve the effects of short reproduction cycle, increased differentiation rate, and reduced costs

Inactive Publication Date: 2013-05-01
WUHAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Guizhou Normal University and others have carried out some research work, but only obtained sagittarius test-tube plantlets and stolons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Using the terminal buds of Baoying Ziyuan Sagittarius (commonly known as "Baolaowu") as an explant, the explants are sterilized, stem tip differentiated, subcultured, and induced to form a test-tube Sagittarius, including the following steps:

[0024] 1) Disinfection treatment of explants: select sagittarius bulbs that are free from diseases and insect pests, after cleaning, cut the bulb terminal buds as explants, rinse with running water for about 30 minutes, filter and dry, and then put them on the ultra-clean workbench to peel off the outermost 2 The scales were disinfected, that is, first treated with 72% streptomycin sulfate for 30 minutes, then sterilized with 70% ethanol for 30 seconds, and finally with 0.1% HgCl 2 Solution treatment for 3 minutes;

[0025] 2) Stem tip differentiation: After the disinfection treatment, the sagittarius was washed 5 times with sterile water, and 3 layers of scales were peeled off with a scalpel, and the shoot tip was stripped until...

Embodiment 2

[0029] Using the terminal buds of Baoying Ziyuan Sagittarius (commonly known as "Baolaowu") as an explant, the explants are sterilized, stem tip differentiated, subcultured, and induced to form a test-tube Sagittarius, including the following steps:

[0030] 1) Disinfection treatment of explants: select sagittarius bulbs that are free from diseases and insect pests, after cleaning, cut the bulb terminal buds as explants, rinse with running water for about 25 minutes, filter and dry, and then put them on the ultra-clean workbench to peel off the outermost 2 The scales were disinfected, that is, first treated with 72% streptomycin sulfate for 20 minutes, then sterilized with 70% ethanol for 40 seconds, and finally with 0.1% HgCl 2 Solution treatment for 5 minutes;

[0031] 2) Stem tip differentiation: After the disinfection treatment, the sagittarius was washed 5 times with sterile water, and 3 layers of scales were peeled off with a scalpel, and the shoot tip was stripped until...

Embodiment 3

[0035] Using the terminal buds of Baoying Ziyuan Sagittarius (commonly known as "Baolaowu") as an explant, the explants are sterilized, stem tip differentiated, subcultured, and induced to form a test-tube Sagittarius, including the following steps:

[0036] 1) Disinfection treatment of explants: select sagittarius bulbs without pests and diseases, clean them, cut bulb terminal buds as explants, rinse with running water for about 35 minutes, filter and dry, and then put them on the ultra-clean workbench to peel off the outermost 1 The scales were disinfected, that is, first treated with 72% streptomycin sulfate for 10 minutes, then 70% ethanol for 30 seconds, and finally 0.1% HgCl 2 Solution treatment 2min;

[0037] 2) Stem tip differentiation: After the disinfection treatment, the sagittaria was washed 5 times with sterile water, and 3 layers of scales were peeled off with a scalpel, and the shoot tip was stripped until only 1 leaf primordium remained, and 0.5 cm of the shoot...

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Abstract

The invention discloses a method for fast propagation of test tube arrowhead by tissue culture. The test tube arrowhead is formed by sterilizing treatment of an explant, stem tip differentiation, subculture multiplication and induction. According to the method disclosed by the invention, the propagation of the test tube arrowhead is carried by tissue culture, and thus high propagation coefficient and short propagation period are realized; in addition, the whole propagation process is carried out under manual control conditions without being influenced by an external environment; the test tube arrowhead can be annually produced; due to small size and light weight, the test tube arrowhead can solve the problems existing in the remote transportation and storage of arrowhead test tube seedlings; the test tube arrowhead has no disease, so that the problem that a conventional arrowhead seed easily carries diseases is solved; the induction of the test tube arrowhead provides an operable simple system for research work of the carmus expansion mechanism; as an in-vitro dormancy organ, the in-vitro indoor preservation research on arrowhead germ plasm resources can be realized; and live exchange of international germ plasm resources is benefited. The invention provides a quick, convenient and effective propagation method for the development of the arrowhead industry.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, and specifically refers to a method for rapid propagation of Sagittarius tissue culture in test tubes. Background technique [0002] Sagittarius is a perennial aquatic herb that is eaten with its bulbs. my country is one of the origins of Sagittarius. It is generally cultivated in the Yangtze River Basin and the provinces to the south. It is mainly produced in the coast of Taihu Lake and the Pearl River Delta, and it is cultivated in a small amount in the north. Sagittarius is mainly propagated asexually by using bulbs or bulb terminal buds of Sagittarius. The reproduction coefficient is low, and only one generation is reproduced a year. The amount of seeds used is large, which limits the promotion speed of new and excellent varieties of Sagittarius. [0003] Therefore, the production of sagittarius in test tube by rapid propagation through tissue culture is one of the effective ways to solve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱红莲柯卫东刘玉平彭静黄新芳刘义满李峰李双梅黄来春叶元英
Owner WUHAN VEGETABLE RES INST
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