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Human mesenchymal stem cell osteogenic differentiation medium and preparation method thereof

A technique for inducing differentiation of mesenchymal stem cells, applied in the field of stem cells, can solve the problems of unclear induction characteristics of human mesenchymal stem cells, weak specificity of directional induced osteogenic differentiation, and unsatisfactory induction and differentiation efficiency, etc., and achieve high-efficiency human mesenchymal stem cells. Effects of osteogenic differentiation, shortening induction time, and improving induction efficiency

Inactive Publication Date: 2020-01-17
安徽瑞杰赛尔生物科技有限公司
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  • Description
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  • Application Information

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Problems solved by technology

This medium is only for bone marrow-derived mesenchymal stem cells to induce osteogenic differentiation, the induction differentiation efficiency is not ideal, and the specificity of directional induction of osteogenic differentiation is not strong, and the induction characteristics of human mesenchymal stem cells are not clear
[0005] In summary, the existing osteogenic differentiation medium cannot efficiently and stably induce the differentiation of human mesenchymal stem cells from various tissue sources into osteoblasts, and more optimized differentiation conditions are still needed to improve the osteogenic differentiation of human mesenchymal stem cells. efficiency, shorten the time of induction and differentiation, and thus provide favorable conditions for the identification of mesenchymal stem cells' osteogenic differentiation ability and the clinical treatment of bone injuries

Method used

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  • Human mesenchymal stem cell osteogenic differentiation medium and preparation method thereof
  • Human mesenchymal stem cell osteogenic differentiation medium and preparation method thereof
  • Human mesenchymal stem cell osteogenic differentiation medium and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Differentiation Induction Medium 1

[0028] A human mesenchymal stem cell osteogenic differentiation medium, including α-MEM / HG-DMEM medium, also includes the following components and their concentrations: fetal bovine serum 10% by volume, ascorbic acid 50μM, phosphate glycerol 10mM, dextrose Methasone 100nM, IGF-11nM and IGF-22nM.

[0029] The preparation method comprises the following steps:

[0030] Preparation of ascorbic acid: take 10 g of ascorbic acid, dissolve it in α-MEM medium, prepare a 5 mM mother solution, and store it at -20°C. Preparation of β-glycerol phosphate: take 10 g of β-glycerol phosphate, dissolve it in α-MEM medium, prepare a 1M mother solution, and store at -20°C. Preparation of dexamethasone: Take 10 g of dexamethasone, dissolve it in 95% ethanol, prepare a 100 μm mother solution, and store it at -20°C. IGF-1 preparation: Take 1 mg IGF-1, dissolve it in α-MEM medium, prepare a 1 μM stock solution, and store it at -20°C. Preparat...

Embodiment 2

[0032] Example 2: Differentiation Induction Medium 2

[0033] A human mesenchymal stem cell osteogenic differentiation medium, including α-MEM / HG-DMEM medium, also includes the following components and their concentrations: fetal bovine serum 15% by volume, ascorbic acid 80μM, glycerol phosphate 20mM, dextrose Methasone 100nM, IGF-15nM and IGF-20.5nM.

[0034] The preparation method is the same as in Example 1.

Embodiment 3

[0035] Example 3: Differentiation Induction Medium 3

[0036] A human mesenchymal stem cell osteogenic differentiation medium, including α-MEM / HG-DMEM medium, also includes the following components and their concentrations: fetal bovine serum 10% by volume, ascorbic acid 50μM, phosphate glycerol 10mM, dextrose Methasone 150nM, Insulin Growth Factor-10.5nM and Insulin Growth Factor-210nM.

[0037] The preparation method is the same as in Example 1.

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Abstract

The invention relates to the technical field of stem cells, and in particular to a human mesenchymal stem cell osteogenic induction differential medium and a preparation method. The human mesenchymal stem cell osteogenic induction differential medium comprises an alpha-MEM / HG-DMEM medium as well as the following components in concentration: 5-50% by volume of fetal calf serum, 10-100mu M of ascorbic acid, 5-50mM of phosphoglycerol, 50-500nM of dexamethasone, 0.1-10nM of an insulin-like growth factor-1 and an 0.5-50nM of insulin-like growth factor-2. By adopting the medium, human mesenchymal stem cell osteogenic induction differential signal activation is intensified through the insulin-like growth factor-1 and the insulin-like growth factor-2, osteoblast proliferation is promoted, then the human mesenchymal stem cell osteogenic induction differential efficiency and specificity are improved, the differential time is shortened, the induction efficiency is improved, meanwhile the preparation method is simple and convenient and convenient to use, and stable and efficient human mesenchymal stem cell osteogenic induction differential can be achieved.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a culture medium for osteogenic induction and differentiation of human mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells, which were first discovered in the bone marrow. Exist in various tissues, such as umbilical cord, fat, dental pulp and other tissues, easy to isolate and amplify. A large number of studies have proved that mesenchymal stem cells have good self-renewal and multi-directional differentiation potential, and can be induced to differentiate into fat cells, bone cells, chondrocytes, muscle cells, liver cells, nerve cells, skin cells, etc. Therefore, mesenchymal stem cells have been tried to be applied to the repair of various tissues and organ functions, and have great potential for clinical application. Among them, the application of bone tissue reconstructio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0654C12N2500/35C12N2500/38C12N2501/105C12N2501/39C12N2506/1353C12N2506/1384C12N2506/1392
Inventor 罗乐朱灏
Owner 安徽瑞杰赛尔生物科技有限公司
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