173 results about "Glycerol phosphate" patented technology

The invention relates to a preparation method of a gel containing stem cell exosomes for repairing skin wounds. The method comprises the following steps: 1) primary extraction and culture of human umbilical cord mesenchymal stem cells: 1.1) primary extraction of human umbilical cord mesenchymal stem cells, 1.2) subculture, and 1.3) collection of the culture supernatant; 2) extraction of human umbilical cord mesenchymal stem cell exosomes: 2.1) primary centrifugation, 2.2) secondary centrifugation, 2.3) removal of organelles by centrifugation, 2.4) coarse extraction of exosomes, and 2.5) finalextraction of exosomes; 3) preparation of a gel material: 3.1) preparation of chitosan, 3.2) configuration of beta-glycerol phosphate (beta-GP), and 3.3) preparation of the gel material; and 4) gel loading of the exosomes. The gel containing stem cell exosomes can promote repair of skin wounds, shorten the healing time of wounds and reduce scar formation.

A one-component self-etching self-priming dental adhesive composition is disclosed. The composition comprises glycerol phosphate di(meth)acrylate monomer, at least one mono-functional polymerizable monomer having just one ethylenically unsaturated group, at least one multi-functional polymerizable monomer having at least two ethylenically unsaturated groups, at least one aprotic solvent, at least one protic solvent, and at least one polymerization initiator.

The invention relates to a novel multivalent carrier vaccine for a shrimp. The vaccine is characterized by comprising a bacillus subtilis recombination strain obtained by a genetic engineering technique, the strain belongs to the category of bacillus subtilis HT5303 and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO.: M 2012395. When spores are formed by the bacillus subtilis vaccine strain, white spot syndrome virus VP281 protein can be demonstrated on the surfaces of the spores, and fusion protein of cell-penetrating peptides, white spot syndrome virus VP19 and vibrio harveyi 3-glyceraldehyde-phosphate dehydrogenase (GAPDH) can be secreted and expressed when vegetative cells are formed. Compared with other vaccines, the multivalent carrier vaccine has the advantages of cross protection and long protection period. Moreover, the vaccine can be added into feeds and has the advantages of resistance against high-temperature setting of feeds and broad prospects for commercial development.

A one-component self-etching self-priming dental adhesive composition is disclosed. The composition comprises glycerol phosphate di(meth)acrylate monomer, at least one mono-functional polymerizable monomer having just one ethylenically unsaturated group, at least one multi-functional polymerizable monomer having at least two ethylenically unsaturated groups, at least one aprotic solvent, at least one protic solvent, and at least one polymerization initiator.

The invention relates to the field of the biotechnology, in particular to an osteogenic induction medium and an osteogenic differentiation method. The osteogenic induction medium is prepared from vitamin C, dexamethasone, beta-sodium glycerol-phosphate, bone morphogenetic protein-2, a vascular endothelial growth factor and a basic medium. Multiple inducing factors in the osteogenic induction medium are combined to act on GMSCs (gingival mesenchymal cells) and have a synergistic effect, the osteogenic differentiation of MSCs can be effectively induced, and the osteogenic differentiation effect is remarkably superior to that of a conventional induction method.

Glycerol-3-phosphate acyltransferase is the initial enzyme of the glycerolipid biosynthetic pathway. Biochemical analyses indicated that the reaction mediated by glycerol-3-phosphate acyltransferase represents a potential rate-limiting step for the synthesis of phospholipids and storage neutralipid, triacylglycerol. The present invention relates to the cloning of genes encoding extraplastidic membrane-bound glycerol-3-phosphate acyltransferases. Heterologous expression of the genes, GPAT1, GPAT2, and GPAT3 in a yeast glycerol-3-phosphate acyltransferase mutant demonstrated that the encoded products could efficiently utilize glycerol-3-phosphate to mediate sn-1 stereo-specific fatty acid acylation. The invention encompasses the glycerol-3-phosphate acyltransferase peptides disclosed and fragments and homologues thereof, the corresponding gene sequences and fragments and homologues thereof, as well as the use of the peptide and gene sequences of the present invention for use in generating recombinant proteins, and transgenic plants with altered lipid metabolism. In this way, the present invention also encompasses the use of such recombinant peptides and transgenic plants for the production of lipid products for use, for example, in pharmaceutical and nutritional applications.

The present disclosure relates to an activated polymer composition for use in repairing tissue of a patient comprising platelet-rich plasma (PRP), a chitosan solution, a salt such as NaCl or glycerol phosphate, and an activator such as at least one of CaCI2 and a mixture of thrombin / CaCI2.

The invention discloses a recombinant bacterium for synthesizing butantriol oleate as well as a construction method and application of the recombinant bacterium, belonging to the technical field of gene engineering. For the recombinant bacterium, escherichia coli is used as an original strain, and a gene for encoding acyltransferase, a gene for encoding acyl CoA synthetase, a gene for encoding enoyl ACP reductase, a gene for encoding glycerol kinase, a gene for encoding 3-phosphoglycerol acyltransferase and a gene for encoding DAGP phosphatase are introduced into the escherichia coli to obtainrecombinant bacterium. Through fermentation, first synthesis of 1,2,4-butantriol oleate is realized. The recombinant bacterium constructed by the invention is fermented in an LB culture medium for 24hours, and the esterification of butantriol and sodium oleate can be realized to obtain the butantriol oleate, so that a new thought is provided for separation and purification of the butantriol.