50 results about "Phosphoglyceric acid" patented technology

The invention relates to a method for the production of plants with suppressed photo-respiration and improved CO2 fixation. In particular, the invention relates to a re-use of phosphoglycolate produced in photorespiration. The reaction product will be converted to a component that may be reintegrated into the plant assimilatory metabolism inside the chloroplast. This is accomplished by the transfer of genes derived from glycolate-utilizing pathways from bacteria, algae, plants and / or animals including humans into the plant nuclear and / or plastidial genome. The method of the invention leads to a reduction of photorespiration in C3 plants and by this will be of great benefit for food production especially but not exclusively under non-favourable growth conditions.

The present invention relates to a strain of Bacillus cereus ( Bacillus cereus strain ) BY1, whose preservation number in the China Type Culture Collection Center is CCTCC M 2017321, the resistance of the BY1 strain of the present invention to formaldehyde on a solid medium can reach 10mM, and in a short period of formaldehyde stress, through ribulose The monophosphate pathway assimilated formaldehyde into glucose 6-phosphate and fructose, and the serine pathway assimilated formaldehyde into serine, glycine, malate, glyoxylate, phosphoenolpyruvate and 3-phosphoglycerate during a long period of formaldehyde stress. These metabolites then enter other metabolic pathways to be converted to glutamine, asparagine, glutamic acid, alanine, acetic acid and ethanol. The resistance to acetaldehyde, ethanol, isopropanol, xylene, benzene, and toluene in a solid medium containing 5mM methanol is 20mM, and it grows better in a culture solution containing high concentrations of benzene series, and can be treated with sodium acetate Or sodium malate or sodium citrate is used as the carbon source to remove the added benzene series, and the removal rate reaches 100%.

The present invention is one kind of thermophilic heat resisting glyceratekinase and its preparation process and application, and belongs to the field of bioengingeering technology. The glyceratekinase has optimal activity temperature of 45 deg.c, relatively wide adapting temperature range, very high activity in 20-50 deg.c and acidic condition, and optimal activity pH of 2.5. The glyceratekinase may have ATP, GTP, CTP, UTP or ADP as phospho donor. The preparation process of the glyceratekinase includes the steps of selecting plasmid, strain and culture medium, PCR amplification and recombinant plasmid constitution, gene inducing expression and protein purification. The glyceratekinase has very high heat stability, and may be used for preparing 2-phosphoglyceric acid in medicine and bioengineering.

The present invention relates to a method for diagnosing peritoneal carcinoses or metastatic primary tumors in a subject, as well as to a method for providing a prognosis to a subject diagnosed with a primary tumor to develop metastases, in particular peritoneal carcinosis, comprising the step of determining the level of expression of at least phosphoglycerate kinase 1 (PGK1) gene. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the activity and / or expression of phosphoglycerate kinase 1 (PGK1) and / or β-catenin, either alone or in combination with the detection of CXCR4 and / or CXCL12, for the diagnosis or prognosis of peritoneal carcinoses and / or metastatic primary tumors. Also, a method for the preventive treatment of peritoneal carcinoses and / or metastatic primary tumors in a subject in need thereof is disclosed, wherein the method comprises the step of administering to the subject at least a pharmaceutically effective amount of a substance inhibiting the activity and / or expression of phosphoglycerate kinase 1 (PGK1).

The invention discloses a blood marker-PGK1 for rheumatoid arthritis clinical diagnosis, and discloses a reagent for rheumatoid arthritis clinical diagnosis. The reagent comprises a PGK1 antibody, an HRP-IgG antibody and a conventional reagent in a blood diagnosis reagent. The conventional reagent in the blood diagnosis reagent comprises a carbonate buffer solution, PBST washing liquor, confining liquid, a developing solution and a stop solution. The prepared detection reagent has the advantages of being high in sensitivity, high in specificity and the like and is capable of being widely applied to preliminary survey and health examination of clinical rheumatoid arthritis and providing a reliable basis for clinical diagnosis.

The invention discloses a D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent, and relates to the technical field of grease adsorbents. The D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent is prepared from the following steps: (1) sodium silicate and sulfuric acid are taken as raw materials, and gelling, acidifying treatment, aging, washing, chambering and drying are performed on the raw materials to obtain silica gel; (2) the silica gel and 3-bromopropanol are reacted to obtain an intermediate I; (3) the intermediate I and D-(-)-3-phosphoglyceric acid are reacted to obtainD-(-)-3-phosphoglycerate chiral molecule bonded grease silica gel; (4) the D-(-)-3-phosphoglycerate chiral molecule bonded grease silica gel is washed, and then is vacuum dried at room temperature toobtain the finished product D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent; and (5) after the finished product D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent passes inspection, packaging and storage are performed on the finished product. The prepared modified grease silica gel adsorbent has high selective adsorption on phospholipid and saponin in grease and the adsorptionrate reaches above 98%.

The invention provides nucleoside high-yielding bacteria and its construction method and application. The present invention also provides a method for fermentatively producing nucleosides, comprising: (1) enhancing the serine hydroxymethyltransferase gene glyA of Bacillus subtilis as shown in SEQ ID NO: 2; and / or (2) weakening the Bacillus subtilis chromosome 2,3-bisphosphoglycerate-independent phosphoglycerate mutase gene pgm encoding NCBI reference sequence WP_003228330.1; and (3) using the strain obtained in step (1) and / or step (2) for nucleoside fermentation production. The bacillus subtilis engineering bacterium provided by the invention is a high-yield nucleoside strain, which can effectively accumulate nucleosides, increase the yield of nucleosides, and lay a foundation for the industrial production of nucleosides.