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A novel bacillus cereus by1 and its application

A new type of Bacillus cereus technology, applied in the field of environmental microorganisms, can solve the problems of unclear composition and complex activated sludge composition.

Active Publication Date: 2020-10-09
YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have used activated sludge (sewage sludge), vermiculite powder and ceramic particles to make bioreactors. The results of the research have confirmed that such bioreactors can effectively remove and degrade formaldehyde and various organic substances polluted in the air, but activated sludge There are many types of microorganisms in the environment, the composition is not clear, and some pathogenic bacteria may be contained
In addition, due to the complex composition of activated sludge, the organic matter contained in it may cause the reproduction and growth of mold under high temperature and high humidity conditions

Method used

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  • A novel bacillus cereus by1 and its application
  • A novel bacillus cereus by1 and its application
  • A novel bacillus cereus by1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening, Identification and Detection of Bacterial Strains

[0021] The present invention first uses alginate and sponge to embed the activated sludge collected in the artificial wetland, uses the air that pollutes high concentration formaldehyde and benzene series in the reagent to treat and domesticate the microorganisms in the sponge to embed the activated sludge, and then utilizes the screening medium Enrich the domesticated microorganisms and isolate the strains that grow fast and have strong resistance to formaldehyde and benzene series. The specific screening steps are:

[0022] 1. Encapsulation of activated sludge and domestication of microorganisms

[0023] The activated sludge collected from the constructed wetland is mixed with water, filtered with gauze to remove large particles such as gravel, mixed with 2% sodium alginate 1:1, soaked in the sponge and removed the excess liquid mixture, and then put in 4% CaCl 2 Solidified embedding in solutio...

Embodiment 2

[0029] Example 2 Analysis of formaldehyde resistance, absorption capacity and metabolic pathway of Bacillus cereus BY1

[0030] 1. Resistance analysis of BY1 to formaldehyde

[0031] Add 4 mM, 8 mM, 12 mM and 20 mM formaldehyde to the solid medium with and without yeast extract, and inoculate the BY1 bacterial solution (1%, v / v) in a liquid inorganic medium containing 5 mM methanol and yeast extract. In the salt culture medium, culture on a shaker at 28°C. When the OD600 reaches 0.5, take 1 μl, 2 μl, 3 μl, 4 μl, 5 μl, 6 μl, 7 μl, 8 μl, and 9 μl of the bacterial liquid and spot it on the solid medium containing different concentrations of formaldehyde, 28 After culturing in an incubator at ℃ for 48 hours, the growth status was observed. The results showed that the strain BY1 could grow in the inorganic medium with and without yeast extract, and the growth conditions were almost the same, indicating that the growth of the strain BY1 did not depend on the yeast extract. With the ...

Embodiment 3

[0037] Example 3 Tolerance of Bacillus cereus BY1 to benzene series and other organic pollutants and analysis of the removal effect of benzene series

[0038] 1. Analysis of tolerance of BY1 to benzene series and other organic pollutants

[0039] Add ethanol, isopropanol, xylene, benzene, and toluene at concentrations of 4 mM, 8 mM, 12 mM, and 20 mM to the solid medium containing 5 mM methanol to prepare solid plates. Take 1 μl, 2 μl, 3 μl, 4 μl, 5 μl, 6 μl, 7 μl, 8 μl, 9 μl of the bacterial solution with an OD600 of 0.5 and spot on a solid plate, and observe the growth after cultivating in a 28°C incubator for 48 hours. The result shows that adding acetaldehyde, ethanol, isopropanol has no obvious effect on the growth of bacterial strain BY1, and its tolerance to acetaldehyde, ethanol, isopropanol can reach 20mM, but growth is slower on the plate containing isopropanol. Condition is poor. It can grow on the plate added with xylene, benzene, and toluene, and the resistance t...

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Abstract

The present invention relates to a strain of Bacillus cereus ( Bacillus cereus strain ) BY1, whose preservation number in the China Type Culture Collection Center is CCTCC M 2017321, the resistance of the BY1 strain of the present invention to formaldehyde on a solid medium can reach 10mM, and in a short period of formaldehyde stress, through ribulose The monophosphate pathway assimilated formaldehyde into glucose 6-phosphate and fructose, and the serine pathway assimilated formaldehyde into serine, glycine, malate, glyoxylate, phosphoenolpyruvate and 3-phosphoglycerate during a long period of formaldehyde stress. These metabolites then enter other metabolic pathways to be converted to glutamine, asparagine, glutamic acid, alanine, acetic acid and ethanol. The resistance to acetaldehyde, ethanol, isopropanol, xylene, benzene, and toluene in a solid medium containing 5mM methanol is 20mM, and it grows better in a culture solution containing high concentrations of benzene series, and can be treated with sodium acetate Or sodium malate or sodium citrate is used as the carbon source to remove the added benzene series, and the removal rate reaches 100%.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a bacillus cereus which has two formaldehyde assimilation pathways and can purify formaldehyde and benzene series pollution. Background technique [0002] With the continuous improvement of the quality of life, decoration has become a part of modern life. Formaldehyde is an adhesive widely used in many decoration materials. Materials using formaldehyde as adhesive more or less contain some uncrosslinked free Formaldehyde, free formaldehyde will be continuously released (up to 5-10 years) from these materials, causing serious indoor air formaldehyde pollution, which has become a serious environmental problem in my country and developing countries. Humans and animals exposed to formaldehyde-polluted air can cause skin inflammation; formaldehyde enters the respiratory system and causes respiratory tract irritation and allergies; it reaches the whole body throug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/085
CPCB01D53/72B01D53/84B01D2251/95B01D2257/708C12N1/205C12R2001/085Y02A50/20
Inventor 陈丽梅李昆志王茹冯永
Owner YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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