37 results about "Serine hydroxymethyltransferase" patented technology

A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3′-phosphoadenosine 5′-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.1-protein, AT1 G53885-protein, AT2G35300-protein, AT3G04620-protein, AT4G01870-protein, AT5G42380-protein, AT5G47440-protein, CDS5394-protein, CDS5401_TRUNCATED-protein, cold response protein, cullin, Cytochrome P450, delta-8 sphingolipid desaturase, galactinol synthase, glutathione-S-transferase, GTPase, haspin-related protein, heat shock protein, heat shock transcription factor, histone H2B, jasmonate-zim-domain protein, mitochondrial asparaginyl-tRNA synthetase, Oligosaccharyltransferase, OS02G44730-protein, Oxygen-evolving enhancer protein, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase family protein, plastid lipid-associated protein, Polypyrimidine tract binding protein, PRLI-interacting factor, protein kinase, protein kinase family protein, rubisco subunit binding-protein beta subunit, serine acetyltransferase, serine hydroxymethyltransferase, small heat shock protein, S-ribosylhomocysteinase, sugar transporter, Thioredoxin H-type, ubiquitin-conjugating enzyme, ubiquitin-protein ligase, universal stress protein family protein, and Vacuolar protein.

Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.

The present invention relates to a method for the treatment of cancer or an autoimmune disorder, comprising the administration of a serine hydroxymethyltransferase (SHMT) inhibitor, and in particular the administration of pyrazolopyran compounds of Formula (VI) as presently described, wherein the compounds are capable of inhibiting a mammalian SHMT, such as human SHMT1 and / or SHMT2. The treatment method further comprises the optional administration of an additional agent as a rescue therapy to reduce toxicity, wherein said agent may be chosen from formate, a formate salt, folinic acid, formate ester, or leucovorin.

Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.

The invention discloses a method for improving dyeing performance of a real silk fabric based on enzymatic modification; serine hydroxymethyltransferase is used for catalyzing glycine in protein fibermacromolecules to be converted into serine, the number of the serine in real silk fiber is increased, phosphorylation of the serine and threonine residues in the real silk fiber is catalyzed by protein kinase A, the cationic dye binding effect is enhanced, and the dyeing performance of the real silk fabric is improved. The method specifically comprises the following steps: carrying out enzymaticconversion of amino acids in real silk; and (2) catalyzing the phosphorylation by the protein kinase A. Compared with the modification and improvement of the electronegativity of the surface of the real silk fiber by applying a chemical method, the method has the advantages of being high in enzyme catalysis efficiency, mild in reaction conditions and obvious in dyeing performance improvement.

The invention relates to a rice serine hydroxymethyl transferase protein and a function of a coding gene thereof, wherein the protein has an amino acid sequence shown in SEQ ID NO:2, and the gene for coding the protein has a nucleotide sequence shown in SEQ ID NO:1. The invention also discloses a plasmid containing the gene, a plant expression vector and a host cell. The invention also discloses a lethal method for the seedling stage of cultivars. The plant expression vector is used for converting plant cells, and the converted plant cells are cultured into plants. In the invention, the site of a new gene cloned in a mottle leaf lethal mutant in the seedling stage can be mutated to obtain a seedling-stage lethal phenotype. The invention has broad application prospects in hybrid seed production and purification as well as seed intellectual property protection.

In some aspects, methods of inhibiting survival or proliferation of a tumor cell are provided, the methods comprising inhibiting the glycine cleavage system (GCS) of the tumor cell. In some aspects, methods of treating a subject in need of treatment for a tumor, the method comprising inhibiting the GCS in the tumor. In some embodiments, the methods comprise contacting a tumor cell or tumor with a GCS inhibitor. In some embodiments, the tumor cell or tumor has elevated expression of serine hydroxymethyltransferase 2 (SH1VIT2). In some aspects, methods of identifying a tumor cell or tumor that is sensitive to inhibiting the GCS are provided, the methods comprising determining whether the tumor cell or tumor overexpresses SHMT2. In some aspects, methods of identifying a candidate anti-cancer agent are provided, the methods comprising identifying or modifying a GCS inhibitor.

The invention provides artemisia apiacea absorbing formaldehyde and a cultivation method of the artemisia apiacea. The artemisia apiacea absorbing formaldehyde contains a Shmt gene and an Fdm gene. The sequence of the Shmt gene is SEQ ID NO: 1 and the sequence of the Fdm gene is SEQ ID NO: 2. According to the invention, a serine hydroxymethyltransferase gene Shmt of pseudomonas putida and the Fdm gene of a formaldehyde dismutase gene are cloned and converted into artemisia apiacea so as to improve synthesis of serine hydroxymethyltransferase and formaldehyde dismutase in artemisia apiacea, so that a genetic engineering artemisia apiacea variety with high content of serine hydroxymethyltransferase and formaldehyde dismutase is obtained, thereby greatly improving the capacity of artemisia apiacea absorbing formaldehyde. Therefore, the artemisia apiacea becomes a plant of eliminating formaldehyde released in home decoration.

The invention provides nucleoside high-yielding bacteria and its construction method and application. The present invention also provides a method for fermentatively producing nucleosides, comprising: (1) enhancing the serine hydroxymethyltransferase gene glyA of Bacillus subtilis as shown in SEQ ID NO: 2; and / or (2) weakening the Bacillus subtilis chromosome 2,3-bisphosphoglycerate-independent phosphoglycerate mutase gene pgm encoding NCBI reference sequence WP_003228330.1; and (3) using the strain obtained in step (1) and / or step (2) for nucleoside fermentation production. The bacillus subtilis engineering bacterium provided by the invention is a high-yield nucleoside strain, which can effectively accumulate nucleosides, increase the yield of nucleosides, and lay a foundation for the industrial production of nucleosides.

The present invention provides a component having effects in vivo of improving or optimizing the intestinal environment, suppressing intestinal putrefaction, or suppressing alternation of intestinal bacterial growth and / or pathological changes of intestinal bacterial growth in gut microbiota. The invention also provides an active ingredient suitably used for treating intestinal diseases. The invention provides a monoclonal IgA antibody that binds to amino acids 11 to 333 of serine hydroxymethyltransferase.

The present invention relates to the field of biotechnology, in particular to a mutant of serine hydroxymethyltransferase and use thereof. The present invention provides a mutant of serine hydroxymethyltransferase. The amino acid sequence of the mutant of serine hydroxymethyltransferase is relative to the amino acid sequence of wild-type serine hydroxymethyltransferase. The amino acid residue at position 229, or the amino acid residue at position 229, is replaced by other amino acid residues, and the amino acid sequence of the wild-type serine hydroxymethyltransferase includes the sequence shown in SEQ ID NO.24. The mutant of serine hydroxymethyltransferase and related genetic engineering bacteria provided by the invention can effectively improve the ability of Escherichia coli to produce L-serine.

The invention discloses a silk antibacterial finishing method based on enzyme-catalyzed conversion and grafting. Glycine in protein fiber macromolecules is catalyzed by utilizing serine hydroxymethyltransferase to be converted into serine, the number of serine in silk fibers can be increased, then the serine in the silk fibers is catalytically oxidized by utilizing laccase and a 2,2,6,6-tetramethyl pyridine-nitrogen-oxide (TEMPO) system to generate an aldehyde group, the aldehyde group is cross-linked with polylysine to perform the silk antibacterial finishing treatment. The silk antibacterial finishing method comprises the following steps: (1) enzymatically converting amino acid in silk; and (2) catalytically oxidizing silk grafted polylysine by virtue of laccase-TEMPO. Compared with thetraditional chemical cross-linking method antibacterial finishing treatment, the silk treated in the method of the invention has good antibacterial performance, mild enzyme treatment condition, and higher physical and mechanical properties compared with an un-treated sample.