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72 results about "Transcription initiation" patented technology

Transcription Initiation. Transcription initiation is a stepwise process including (1) promoter binding, (2) promoter melting, (3) promoter release (preceded by 9nt RNA synthesis), and (4) RNA extension of initial transcripts (up to 14nts) to allow the formation of stable elongation complex.

Processes and vectors for producing transgenic plants

A process for producing transgenic plants or plant cells capable of expressing a coding sequence of interest under transcriptional and translational control of host nuclear transcriptional and translational elements is described by introducing into the nuclear genome of host plants or plant cells a vector comprising said coding sequence of interest which is devoid of (a) an upstream element of initiation of transcription functional in the host plants or plant cells and operably linked to said coding sequence of interest and required for its transcription; (b) an upstream element of initiation of translation functional in the host plants or plant cells and operably linked to said coding sequence of interest; and subsequently selecting plant cells or plants expressing said coding sequence of interest.
Owner:ICON GENETICS

High-efficiency artificial activating transcription factor dCas9-TV, and coding gene and applications thereof

ActiveCN107722125ATranscriptional activationEliminate the need for cloningHydrolasesAntibody mimetics/scaffoldsMetaboliteBiological activation
The invention relates to a high-efficiency artificial activating transcription factor dCas9-TV, and a coding gene and applications thereof. According to a construction method, the carboxyl terminal ofnuclease inactivated Cas9 protein (dCas9) is connected with a plurality of copies of VP64 and TAL transcription-activating domains so as to obtain a series of novel artificial activating transcription factors, and obtain dCas9-TV with the best transcriptional activation activity via screening. When only one guide RNA (gRNA) is adopted for targeting a specific gene promoter, dCas9-TV is capable ofrealizing high efficiency activating of transcription of endogenous genes of Arabidopis thaliana and paddy rice; when a plurality of gRNA are adopted for targeting a plurality of target genes, dCas0-TV is capable of realizing transcription activation of a plurality of genes. In addition, it is confirmed that dCas9-TV possesses the same high efficiency targeting transcription activation activity in human cells. An in vitro assembled dCas9-TV/gRNA ribonucleoprotein compound can be adopted for transcription activation of Arabidopis thaliana and paddy rice endogenous genes. The high-efficiency artificial activating transcription factor dCas9-TV can be adopted in the fields such as genome genetic screening, metabolite biosynthesis pathway reconstruction, and crop improvement.
Owner:SUN YAT SEN UNIV

Higher order structure and binding of peptide nucleic acids

Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.
Owner:IONIS PHARMA INC +1

Genes coding for tomato beta-galactosidase polypeptides

A novel beta -galactosidase gene family and DNA sequences derived from the cloning of cDNAs encoding products of these genes are provided, as exemplified by a beta -galactosidase II protein which is encoded by a cDNA clone, pZBG2-1-4. A method for modifying cell wall metabolism which involves modifying the activity of at least one beta -galactosidase, and thus modifying the quality of the fruit is also provided. Also provided by the present invention is a DNA construct including some or all of a beta -galactosidase DNA sequence under control of a transcriptional initiation region operative in plants, so that the construct can generate RNA and, optionally, beta -galactosidase polypeptide in plant cells. The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells and for using them for production of beta -galactosidase polypeptides or peptides by recombinant techniques. The present invention also provides plant cells containing DNA constructs of the present invention; plants derived therefrom having modified beta -galactosidase gene expression; and seeds produced from such plants.
Owner:US SEC AGRI

Short exogenous promoter for high level expression in fungi

Provided herein are short exogenous fungi transcription promoter nucleic acid sequences and methods of using the exogenous fungi transcription promoter nucleic acid sequences to modulate transcription initiation or rate of transcription.
Owner:BOARD OF RGT THE UNIV OF TEXAS SYST

Tumor-specific promoters

InactiveUS20030157065A1High tumor-specificityHigh promoter activityBiocideGenetic material ingredientsPromoter activityC erbb 2
A DNA comprising a 609 bp base sequence from -559 to +50 when the first base sequence of exon 1 of the midkine gene, a human retinoic acid-responsive growth / differentiation factor was set as +1, or a DNA comprising a 251 bp base sequence from -213 to +38 when the transcription initiation point of the c-erbB-2 gene belonging to the EGF receptor family and having a tyrosine kinase activity was set as +1 has a tumor-specific transcription activity, and the promoter activity thereof is high, and therefore is very important as a tumor-specific promoter for use in the suicide gene therapy that combines the use of a gene for a drug metabolizing enzyme and a prodrug for cancer therapy, the gene therapy of cancer using an expression vector that contains a gene encoding a cytokine, and the gene therapy of cancer using an oncolytic virus that exhibits cytotoxic effects only on tumor cells, etc.
Owner:CHIBA PREFECTURE +1
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