The invention relates to a detection primer, detection kit and detection method of dehiscence resistance of Sesamum indicum, and belongs to the technical field of molecular biology. Compared with the sequence of a dehiscent Sesamum indicum gene, in the KANADI gene of anti-dehiscent Sesamum indicum, 14 bases are deleted in the DNA sequence, 77 bases are deleted in the cDNA sequence, the deleted bases are located between the 1,118th bp and the 1,332th bp in the downstream position of a transcription initiation codon, and the 14 bp bases are located in the connection position of a second exon and a second intron. According to the feature of the gene sequence differences, a detection primer of dehiscence resistance is designed, an important detection primer is provided for molecular marker assisted selection breeding of Sesamum indicum, and a rapid and accurate detection method is provided for screening of a new variety of Sesamum indicum with dehiscence resistance.