Genes coding for tomato beta-galactosidase polypeptides

A galactosidase and coding technology, which is applied in the fields of glycosylase, genetic engineering, plant gene improvement, etc., can solve the problems such as difficult to obtain gene cloning

Inactive Publication Date: 2001-09-05
US SEC AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the β-galactosidase II protein present in ripe tomato (Lycopersicon esculentum Mill.) fruit and capable of degrading tomato fruit galactan has been purified, it is still difficult to obtain the cloning of the corresponding gene

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  • Genes coding for tomato beta-galactosidase polypeptides
  • Genes coding for tomato beta-galactosidase polypeptides
  • Genes coding for tomato beta-galactosidase polypeptides

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Embodiment Construction

[0035] The following detailed description relates to preferred embodiments of the invention and is intended to illustrate each of the other DNA sequences of the invention.

[0036] The present invention provides isolated nucleic acid molecules comprising a polynucleotide encoding a β-galactosidase polypeptide, particularly a β-galactosidase II polypeptide having the amino acid sequence shown in FIG. 2 . The DNA sequence of the exemplary β-galactosidase II cDNA clone of the present invention is determined from the cDNA clone pZBG2-1-4 encoding β-galactosidase II, which is included in GenBank with the accession number AF020390. Not all β-galactosidases are active in vitro on the release of galactose from cell wall polymers comprising β(1→4)-D-galactan by extracted cell wall material. It has been shown that the polypeptide expressed by the exemplary β-galactosidase II clone pZBG2-1-4 has β-galactosidase activity and exogalactinase activity.

[0037] As shown in Figure 2, the exe...

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Abstract

A novel beta -galactosidase gene family and DNA sequences derived from the cloning of cDNAs encoding products of these genes are provided, as exemplified by a beta -galactosidase II protein which is encoded by a cDNA clone, pZBG2-1-4. A method for modifying cell wall metabolism which involves modifying the activity of at least one beta -galactosidase, and thus modifying the quality of the fruit is also provided. Also provided by the present invention is a DNA construct including some or all of a beta -galactosidase DNA sequence under control of a transcriptional initiation region operative in plants, so that the construct can generate RNA and, optionally, beta -galactosidase polypeptide in plant cells. The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells and for using them for production of beta -galactosidase polypeptides or peptides by recombinant techniques. The present invention also provides plant cells containing DNA constructs of the present invention; plants derived therefrom having modified beta -galactosidase gene expression; and seeds produced from such plants.

Description

field of invention [0001] The present invention relates to a novel family of plant genes encoding polypeptides characterized by their ability to hydrolyze the terminal non-reducing 3-D-galactosyl residues of β-D-galactosides. More specifically, the polynucleotide sequence obtained from the cDNA clone designated pZBG2-1-4 (referred to as pTomβgal4 in U.S. Provisional Application No. 60 / 088,805), which encodes a unique enzyme called β-galactosidase II, is provided. plant peptides. Also provided are cDNA clones encoding six other homologous polypeptides, methods of using these cDNA clones to produce the β-D-galactoside polypeptides of the invention, and methods of improving fruit quality by using the polynucleotides or polypeptides of the invention. Background of the invention [0002] The most significant and important manipulations involved in the post-collection quality of peak respiratory fruit are the changes in structure, colour, taste and aroma tha...

Claims

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Application Information

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IPC IPC(8): A01H5/00C07K16/40C12N5/10C12N9/38C12N15/09
CPCC12N9/2471C12Y302/01023
Inventor K·C·格罗斯D·L·史密斯
Owner US SEC AGRI
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