86 results about "Cis acting" patented technology

The present application describes isolated nucleic acids that contain a Nic gene product responsive element, and the use thereof in methods of producing transgenic tobacco plants having reduced levels of nicotine and / or TSNA therein, as well as other plants or host cells that contain altered levels of a protein of interest therein due to inclusion of a cis-acting decoy element therein.

The invention relates to a new gene for the rice zinc-finger protein transcription factor and the application thereof to drought resistance and salt tolerance, in particular to the rice zinc-finger protein transcription factor, a coding sequence thereof, a carrier or host, a cis-acting element and antagonist of the rice zinc-finger protein transcription factor or the coding sequence. The rice zinc-finger protein transcription factor comprises polypeptide, and the polypeptide can be the polypeptide having the amino acid sequence of SEQ ID NO: 2, the conservative variant polypeptide or the homologous polypeptide separated from the same; the carrier or host includes the coding sequence; and the cis-acting element is combined with the rice zinc-finger protein transcription factor. The invention also relates to a method for improving the drought resistance and the salt tolerance of the rice and a method for sieving the rice with high drought resistance and salt tolerance. The novel method for improving and studying the drought resistance and the salt tolerance of the rice has wide application prospect.

A novel nucleic acid construct for down-regulating angiogenesis in a tissue of a subject is provided. The nucleic acid construct includes: (a) a first polynucleotide region encoding a chimeric polypeptide including a ligand binding domain fused to an effector domain of an apoptosis signaling molecule; and (b) a second polynucleotide region encoding a cis acting regulatory element being for directing expression of the chimeric polypeptide in a specific tissue or cell; wherein the ligand binding domain is selected such that it is capable of binding a ligand present in the specific tissue or cell, whereas binding of the ligand to the ligand binding domain activates the effector domain of the apoptosis signaling molecule. Also provided are methods of utilizing this nucleic acid construct for treating diseases characterized by excessive or aberrant neo-vascularization or cell growth.

The invention discloses an induced slow virus expression system which comprises a target gene expression cassette and an rtTA expression cassette. The target gene expression cassette comprises an induced promoter containing a tetracycline cis-acting response element and a target gene, and the rtTA expression cassette comprises a promoter, an rtTA, a linker peptide and a screening gene, wherein a P2A linker peptide is adopted as the linker peptide, the screening gene is Puro, and the induced promoter is TetO6 or TRE3G. The invention further discloses a construction method of the induced slow virus expression system. The construction method comprises the steps of pLVX-Puro carrier reconstructing; TetON3G gene synthesizing and cloning; pLVX-rtTA3 constructing; EF1a promoter subcloning; red fluorescence protein (RFP) subcloning; subcloning of the induced promoter TRE3G or TETO6 containing the tetracycline cis-acting response element. The induced slow virus expression system can carry out inducible expression of the target gene to the greatest level while keeping low background expression after cells are introduced into the system, is sensitive in response for an inducer, high in efficiency and low in molecular weight and can be widely applied to gene function research.

A recombinant plasmid or expression vector comprising a sequence encoding a trans-acting hairpin ribozyme or inserted RNA flanked by 5′ and 3′ self-cleavage cis-acting hairpin ribozymes, which produces a long RNA transcript that undergoes self-catalyzed cleavage at the 5′ and 3′ sides of the trans-acting ribozyme or inserted RNA.

An extensible RNA-based framework for engineering ligand-controlled gene regulatory systems, called ribozyme switches, that exhibit tunable regulation, design modularity, and target specificity is provided. These switch platforms typically contain a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of a hammerhead ribozyme sequence. A variety of modes of standardized information transmission between these domains can be employed, and this application demonstrates a mechanism that allows for the reliable and modular assembly of functioning synthetic hammerhead ribozyme switches and regulation of ribozyme activity in response to various effectors. In some embodiments aptamer-regulated cis-acting hammerhead ribozymes are provided.

Plant expression construct are provided. According to an embodiment, the plant expression construct comprises a nucleic acid sequence encoding a hexokinase under a transcriptional control of a guard cell-specific cis-acting regulatory element. Also provided are methods of using the constructs and transgenic plants, plant cells and plant parts expressing same.