98 results about "Cis-regulatory element" patented technology

Compositions, methods and kits are provided that are useful, for example, for determining activities of multiple cis-regulatory sequences, such as promoters and enhancers, and/or multiple trans-acting factors, such as transcription factors, in a cell. In particular, in certain embodiments, compositions are provided comprising a population of polynucleotide reporter transcription units (RTUs) in which each RTU comprises a reporter sequence, a processing tag located in the reporter sequence; and a cis-regulatory element operably linked to the reporter sequence, wherein the reporter sequences between any two RTUs in the population, outside of the processing tags, are substantially identical and wherein the positions of the processing tags within the reporter sequences distinguish between any two RTUs differing, for example, in their cis-regulatory elements. The compositions, methods and kits can further be used, for example, to identify a cell type or disease state, for example, in a biological organism.

A method for using the promotor LEAP of rice's drought inducting gene to improve the resistance of plant to drought features that the promotor LEAP of rice OsLEAl gene is cloned. Its nucleotide sequence is shown by SEQ ID No.1 and is used to code a drought induced expression protein. Two cis-acting elements ABRE contained in its promotor region can be specifically response to drought and ABA. Its inductive expression carrier can stongly induce the expression of target gene when the drought stress is applied to a plant. The method for culturing the drought-resistant plant is also disclosed.

The invention discloses a promotor CPIP from Oryza Sativa L in the plant genetic engineering domain, which is characterized by the following: the promotor CPIP possesses SEQ ID NO: 1 with nucleic acid sequence, which controls two alfapsin inhibition sub-genes simultaneously; the alfapsin inhibition sub-gene is controlled by promotor CPIP in backward-forward direction, which makes answering reaction with arid, salt-forced and abscisic acid(ABA); the promotor region contains a plurality of hostile environment answering cis-form appliance elements; the promotor CPIP controls GUS report gene in backward-forward direction to converse rice; the GUS gene is induced and expressed strongly in the condition of arid and salt-forced.

The invention discloses an ERF transcription factor related to the plant stress resistance and an encoding gene and application thereof. The invention firstly provides an ERF transcription factor gene LcERF056 separated from lotus japonicas, the amino acid sequence of the gene LcERF056 is shown as SEQ ID No.1, and the nucleotide sequence of the encoding transcription factor is shown as SEQ ID No.2. The invention further provides an ERF transcription factor structure domain which can be combined with a cis-acting element to start stress resistance response gene expression and is shown as SEQ ID No.3. Function analysis experiments prove that the resistance of a plant to high salt stress can be effectively enhanced or improved by overexpressing the LcERF056 gene in the plant, and therefore the protein encoded by the LcERF056 gene has the functions of the EFR transcription factor. The invention further provides application of the ERF transcription factor and the encoding gene on the aspects of enhancing the adversity stress resistance of the plant, breeding new transgenic plant varieties with the adversity stress resistance and the like.

The invention discloses a corn callus specific promoter and a cloning method and application thereof. A WRKY gene is obtained by utilizing bioinformatics analysis, the sequence comparison of the gene and the genome is performed, a primer is designed to perform polymerase chain reaction (PCR) and obtain the promoter region of the gene 5', the promoter is 2880bp and has the sequence shown in the (400)1 item of the sequence table; the analysis of the promoter shows that the promoter contains a plurality of acting elements; and the promoter is subcloned to the vector with GUS gene, expression of the GUS gene is started, and the vector is transformed in rice. The staining examine of each tissue of rice shows that the specificity of the promoter can be expressed in callus, thus the promoter hasimportant application value in genetic engineering and the gene function researches.

The invention discloses a promoter. The promoter can regulate and control the specific expression of a gene in secretion-type glandular hair, and a nucleotide sequence of the AaALDH1 gene promoter is shown as SEQ ID NO.1. The invention also provides an application of the promoter in the genetic engineering breeding by adopting plant secretion-type glandular hair tissues to express and produce metabolite. In addition, the invention also discloses a carrier and an expression cassette containing the promoter. By adopting the AaALDH1 gene promoter, a foundation is set for researching the expression regulation and control of the AaALDH1 gene and analyzing a cis acting element on the promoter, and the important significance on the genetic engineering breeding utilizing the plant secretion-type glandular hair tissues to express and produce the metabolite also can be realized.

The invention discloses a lotus corniculatus AP2/ERF transcription factor and an encoding gene and application thereof and belongs to the field of AP2/ERF transcription factors and application. The invention discloses the gene of an AP2/ERF transcription factor separated from lotus corniculatus. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The amino acid sequence of protein encoded by the gene of the transcription factor is shown as SEQ ID NO.2. The invention further discloses an AP2/ERF transcription factor structural domain which can be combined with a cis-acting element to start adverse-resistant response gene expression, and the amino acid sequence of the structural domain is shown as SEQ ID NO.3. Function conversion experiments prove that the gene of the AP2/ERF transcription factor in a plant can improve salt tolerance of the transgenic plant remarkably, and it shows that the protein encoded by the gene of the transcription factor has a function of the AP2/ERF transcription factor. The gene of the AP2/ERF transcription factor has an important application prospect in improving resistance of the plant to adversity stress.

The invention discloses a cotton fiber specificity promoter GhFLA1 and a gene complete sequence thereof, relating to an important regulatory element related to cotton fiber development. The FLA1 gene complete sequence has totally 1920bp and comprises a 5' end promoter and a gene coding sequence. The gene does not contain an intron and encodes an arabinogalactan protein (AGP). The cotton fiber contains a great amount of the AGP protein, and that AGP irreversible inhibitor can inhibit fiber elongation shows that the protein has an important role for cotton fiber development. That the FLA1 gene mRNA has a great amount of specificity accumulation in the process of cotton fiber cell development shows that the gene promoter plays an important role in the regulation of fiber specificity expression. Experiments also show that the promoter has strong expression activity in tobacco leaf epidermal hair, which further proves that the promoter is the specificity regulatory element of the cotton fiber. The promoter can be used as a gene cis-regulatory element to be applied to cotton molecular breeding research so as to improve the cotton fiber quality and to enhance the cotton yield.

The invention discloses a soybean low-temperature inducing promoter, a recombinant expression vector containing the same, an application of the soybean low-temperature inducing promoter and particularly relates to the field of plant genetic engineering, in particular to expression of a soybean gene promoter sequence can serve as the promoter to control gene under low-temperature stress with an aim to effectively solve the problem that the soybean low-temperature inducing promoter is not developed yet. The promoter is derived from a promoter sequence of soybean ethylene response factor gene GmERF9, the promoter sequence is named as GmERF0P with a base sequence indicated as SEQ ID No: 1; the sequence contains multiple stress-related cis-acting elements; the cis-elements are respectively: GT-1, BIHD10S, WRKY, MYB, MYC and G-box. The soybean low-temperature inducing promoter is used for inducing cold-resistance gene expression under low temperature.

The invention discloses a newly found DNA sequence of the promoter of the chalcone synthase (chs) gene and the manufacturing method and use for the same. The promoter is used for PCR with a joint from sorbonne, and clones 899bp of the upstream regulation sequence of chs gene by combining the characteristics of the nested PCR, TD PCR, hot-started PCR and two-step PCR. The analysis in the promoter database Plant CARE shows that, the sequence has a basically conservative region TACPyAT which expresses specifically in flowers, and has the main cis-acting elements with which the primary promote is provided such as a transcription initiation related TATA box, a transcription auxiliary CAAT box, a G box and CCAAT etc. The promoter can regulate the specific expression of the target gene in the plant, and can provide an effective promoter element for the constructing the plant expression vector, increase the expression of foreign gene, and minimum the biological energy consumption.

The invention discloses the application of berberine in medicament for treating hepatitis B virus infection. In the invention, adopting an HepG2.2.15 cell model for the in vitro selection of anti-hepatitis B virus medicine, preliminarily selecting the effect of the medicament by measuring the difference of HBeAg and HBsAg expression volumn in supernatant fluid of the cell after administration, andfurther determining the effectiveness of the medicine by studying the inhibitory effect of the HBV DNA reproduction level (ccc DNA) in the HepG2.2.15 cell by the medicament. Meanwhile, the influenceon the reproduction of the hepatitis B virus by the transcriptional level of the medicine is studied according to the activity inhibitory effect to HBV viral promoters by the medicine, and key HBV cis-regulatory elements sensitive to the antivirus medicine are found. A pivotal cellular signal transduction pathway possibly participating the transcription reproduction for inhibiting viruses by the medicine is analyzed and verified on the basis, the antivirus molecular mechanism of the medicament is further studied, and the medicament is indicated to be developed and applied to clinical treatmentas an anti-hepatitis B effective medicament.

The invention discloses a subsequence of Ammopiptanthus mongolicus PeNAC1 gene 1217bp promoter, which is named as PeNAC1P. A PLACE database can be used for analyzing and predicting cis acting elements which are contained in PeNAC1P and are relevant to the induction of drought, high salts, ABA, GA, JA, SA, MeJA and other hormones, elements relevant to bacterial and salts, cis elements responding to calcium ions and cis elements participating in the sugar signal transfer. Analyzed by a transient expression experiment, the activity of the PeNAC1P promoter is induced by the salts, GA and IAA. The Ammopiptanthus mongolicus PeNAC1P promoter provided by the research belongs to an induction type promoter and provides an alternative promoter for generating a normal tolerant transgene plant on the basis of researching the function.

The present invention discloses a method for identifying banana aquaporin gene promoter transcription factors. The method comprises the following steps: S1, cloning and element analysis of a MaPIP1;1promoter are conducted: amplification is conducted according to primers of existing genome designed promoters to an amplification length of 1,362 bp, cis-acting elements are analyzed by plant care andPLACE, and results show that there are 72 cis-acting elements in the 1,362 bp promoter sequence. A core region of the MaPIP1;1 promoter is recognized by a yeast one-hybrid method, the promoter core region M-P2 is used as a bait segment to construct a bait vector, an action mechanism of a MaPIP1;1 gene against drought stress is clarified, and the transcription factors directly binding with the MaPIP1;1 promoter under the drought stress condition are directly identified in plants for the first time.

The invention relates to a rice root tip specific expression promoter Pro-Os04g24469 and application thereof. The promoter has a nucleotide sequence shown as SEQ ID NO:1 or a nucleotide sequence which is derived by replacing or deleting one or more nucleotide from the nucleotide sequence and/or adding one or more nucleotide into the nucleotide sequence and has the same function as the nucleotide sequence shown as SEQ ID NO:1. The invention also provides a carrier, a transgenic cell line and engineering bacteria which contain the promoter and provides a transgenic line with the promoter. The invention provides the application of the rice root tip specific expression promoter Pro-Os04g24469 in regulating and controlling downstream genetic expression. The rice root tip specific expression promoter Pro-Os04g24469 has the beneficial effects that the new rice root tip specific expression promoter is provided for the first time, a foundation is laid for further analysis on cis acting element of root specific expression in following steps, and a new promoter element can also be provided for plant root system development and transformation and reconstruction of root configuration as well as plant stress resistance gene engineering and study on genetic improvement.