The invention discloses a method for determining content of 12 residual metals in medicines and in particular relates to the technical field of determination of residual metals in medicines. The method specifically comprises the following steps: S1, taking 12
metal element standard substances, taking a 5%HNO3-2%HCl solution as a
solvent, preparing a reference solution, taking to-be-detected medicines, and dissolving with 5%HNO3-2%HCl to obtain a
test solution; S2, preparing two parts of reference solutions in parallel, taking the 5%HNO3-2%HCl solution as a blank control, performing ICP-MS (
Inductively Coupled Plasma Mass Spectrometry) to respectively obtain response values of the 12
metal elements in the reference solution, and making a
standard curve corresponding to each element; and S3,taking the
test solution, taking the 5%HNO3-2%HCl solution as the blank control, performing ICP-MS detection to obtain the
peak area of the
test solution, and calculating the content of each
metal element in the test solution by a
response factor. According to the method disclosed by the invention, the sample pretreatment is simple and convenient, the time is saved, risk is greatly reduced, the detection time is saved, and the detection efficiency is improved.