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Detection method for content of sanshool in Chinese prickly ash extract

A technology of Zanthoxylum bungeanum extract and Zanthoxylum bungeanum, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the instability of Zanthoxylum bungeanum standard products, achieve accurate correspondence, ensure accuracy, and realize the effect of standardization

Active Publication Date: 2018-04-27
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a detection method for the content of anthocyanin in the pepper extract, which can ensure the stability and accuracy of the detection, and solve the problem of the instability of the anthocyanin standard product. The method is simple to operate, The standardization of detection methods has been achieved

Method used

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  • Detection method for content of sanshool in Chinese prickly ash extract
  • Detection method for content of sanshool in Chinese prickly ash extract
  • Detection method for content of sanshool in Chinese prickly ash extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Preparation of methanol solution containing 2,6-di-tert-butyl-4-methylphenol (BHT)

[0039] Weigh 1.25g of 2,6-di-tert-butyl-4-methylphenol (BHT) reagent into a 250mL volumetric flask, add 100mL of 52°C hot methanol, and ultrasonically dissolve. After cooling to room temperature, the volume was constant with methanol to obtain a methanol solution containing 0.5% of 2,6-di-tert-butyl-4-methylphenol (BHT).

[0040] 2) Standard sample preparation

[0041] Take by weighing 200 mg of synthetic capsaicin standard substance with a purity of 97%, and 9.68 mg of a hydroxy-α-sanshool reference substance with a purity of 95%, place it in the same 100mL volumetric flask, and use 2,6-di-tert-butyl- The methanol solution of 4-methylphenol (BHT) was dissolved to a constant volume to obtain a mixed standard solution of synthetic capsaicin and hydroxy-α-sanshool. The synthetic capsaicin concentration of this mixed standard solution is 1.94mg / mL, and the hydroxyl-α-sanshool concentr...

Embodiment 2

[0074] 1) Preparation of methanol solution containing 2,6-di-tert-butyl-4-methylphenol (BHT)

[0075] Weigh 0.25g of 2,6-di-tert-butyl-4-methylphenol (BHT) reagent into a 250mL volumetric flask, add 100mL of 50°C hot methanol, and ultrasonically dissolve. After cooling to room temperature, the volume was constant with methanol to obtain a methanol solution containing 0.1% of 2,6-di-tert-butyl-4-methylphenol (BHT).

[0076] 2) Standard sample preparation

[0077] Take by weighing 200 mg of synthetic capsaicin standard substance with a purity of 98%, and 9.48 mg of a hydroxy-α-sanshool reference substance with a purity of 97%, place it in the same 100mL volumetric flask, and use 2,6-di-tert-butyl- The methanol solution of 4-methylphenol (BHT) was dissolved to a constant volume to obtain a mixed standard solution of synthetic capsaicin and hydroxy-α-sanshool. The synthetic capsaicin concentration of this mixed standard solution is 1.96mg / mL, and the hydroxyl-α-sanshool concentr...

Embodiment 3

[0102] 1) Preparation of methanol solution containing 2,6-di-tert-butyl-4-methylphenol (BHT)

[0103] Weigh 2.5g of 2,6-di-tert-butyl-4-methylphenol (BHT) reagent into a 250mL volumetric flask, add 100mL of hot methanol at 60°C, and ultrasonically dissolve it. After cooling to room temperature, the volume was constant with methanol to obtain a methanol solution containing 1.0% of 2,6-di-tert-butyl-4-methylphenol (BHT).

[0104] 2) Standard sample preparation

[0105] Take by weighing the synthetic capsaicin standard substance 195mg of purity 98%, take by weighing the hydroxyl-α-sanshool reference substance 9mg of purity 98%, be placed in the same 100mL volumetric flask, use 2,6-di-tert-butyl-4 The methanol solution of -methylphenol (BHT) was dissolved to a constant volume to obtain a mixed standard solution of synthetic capsaicin and hydroxyl-α-sanshool. The synthetic capsaicin concentration of this mixed standard solution is 1.91mg / mL, and the hydroxyl-α-sanshool concentrat...

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Abstract

The invention discloses a detection method for content of sanshool in a Chinese prickly ash extract and belongs to the technical field of foods. The detection method includes the steps of: detecting ato-be-detected sample solution and a synthesized capsicine standard solution with a liquid chromatograph to obtain peak areas, and calculating the content of the sanshool in the to-be-detected sampleaccording to the equation Xi = (Si / S0)*(C0 / f)*d / m, wherein Xi is content of any one sanshool component in the sample, Si is the peak area of the any one sanshool component in the sample, S0 is the peak area of the synthesized capsicine, C0 is the concentration of the synthesized capsicine, f is relative responding factor of hydroxyl-alpha-sanshool relative to the synthesized capsicine, d is diluting ratio of the sample, and m is the weight of the sample. The detection method can guarantee stability and accuracy, solves a problem of instability of a sanshool standard substance, is simple in operation and achieves standardization of detection.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for active ingredients in food additives, and belongs to the field of food detection. Background technique [0002] Zanthoxylum bungeanum is a traditional medicinal and edible seasoning in my country, and it is one of the "eight flavors". Zanthoxylin refers to a series of unsaturated amides, mainly including hydroxy-ε-sanshool, hydroxy-α-sanshool, hydroxy-β-sanshool and hydroxy-γ-sanshool. The content of these four components can be Occupy more than 98% of the total pepperocannin content. [0003] The level of xanthanin content is an important index to evaluate the quality of pepper and its products, but the molecular structure of the standard xanthoxylum is extremely unstable, and it is easily oxidized in contact with air, and it will be lost in a short time when exposed to the air. It is cumbersome, and there is no commercial standard product at present, and it is impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/86
CPCG01N30/02G01N30/34G01N30/8634
Inventor 杨清山蔡荣华武亚明刘登帅栗星
Owner CHENGUANG BIOTECH GRP CO LTD
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