38 results about "Chlorophyll degradation" patented technology

The invention relates to a new key gene participating in the regulation and the control of chlorophyll degradation and application thereof, belonging to the technical field of plant gene engineering. Stay-green traits can prolong the commodity price service life of green leafy vegetables and the postharvest green period of fodder crops so as to increase the content of main nutrition constituents, i.e. chlorophylls and proteins; and the stay-green traits can also outstandingly improve the green period and the landscape effect of lawn plants. The invention provides the key gene AtCRN1 for regulating and controlling the chlorophyll degradation and metabolism of plants. An amino acid coding sequence of the key gene AtCRN1 is characterized by being SEQ ID No: 2. The invention also provides a method for establishing a stay-green plant strain, comprising the following steps of: inducing the gene AtCRN1 of a target plant to generate mutation through chemical or physical factors; and destroying or reducing the expression of the gene AtCRN1. Besides, detecting whether the gene AtCRN1 with overall length is contained in a plant genome or not can also be used as a molecular auxiliary breeding method for screening / identifying the stay-green plant strain.

The invention belongs to the technical field of plant genetic engineering and particularly discloses a chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as an encoding gene and application thereof. The chlorophyll katabolism control associated protein provided by the invention is sourced from the non-heading Chinese cabbage with the variety of 'Xiadongqing',and has the name of BcNYE1 and the amino acid sequence shown as SEQIDNO:2. The encoding gene of the chlorophyll katabolism control associated protein of the non-heading Chinese cabbage has the nucleotide sequence shown as SEQIDNO:1 or the polynucleotide sequence of the amino acid sequence with the code of SEQIDNO:2. The gene disclosed by the invention can be applied to chlorophyll katabolism molecular mechanism research of the non-heading Chinese cabbage and improvement of plant stay-green property on the aspects of delaying blade chlorophyll degradation and blade senescence of plants.

The invention relates to a new key gene participating in the regulation and the control of chlorophyll degradation and application thereof, belonging to the technical field of plant gene engineering. Stay-green traits can prolong the commodity price service life of green leafy vegetables and the postharvest green period of fodder crops so as to increase the content of main nutrition constituents, i.e. chlorophylls and proteins; and the stay-green traits can also outstandingly improve the green period and the landscape effect of lawn plants. The invention provides the key gene AtCRN1 for regulating and controlling the chlorophyll degradation and metabolism of plants. An amino acid coding sequence of the key gene AtCRN1 is characterized by being SEQ ID No: 2. The invention also provides a method for establishing a stay-green plant strain, comprising the following steps of: inducing the gene AtCRN1 of a target plant to generate mutation through chemical or physical factors; and destroying or reducing the expression of the gene AtCRN1. Besides, detecting whether the gene AtCRN1 with overall length is contained in a plant genome or not can also be used as a molecular auxiliary breeding method for screening / identifying the stay-green plant strain.

The invention discloses a metal complex of chlorophyll degradation product chlorin e6 derivatives as well as a preparation method and applications of the metal complex. The metal complex of the chlorin e6 derivatives is shown in the formula I (shown in the specification), wherein R1 and R2 are H or CH3, CH2CH3, CH2CH2CH3, n-C4H9 and other common alkane groups, M is common divalent metal ions such as Cu<2+>, Zn<2+>, Fe<2+>, Mn<2+>, Ni<2+>, Co<2+> and Pd<2+>. The compound is clear in structure and excellent in activity, and has potential for developing anti-gastric ulcer and liver protection drugs. The metal complex is low in sources of raw materials, the preparation method is simple and fast to react, and suitable for large-scale industrial production. The formula is shown in the specification.

The invention discloses a cut flower preservative. The preservative comprises the following components in parts by weight: 100-400 parts of inorganic salt, 10-20 parts of a bacteriostatic agent, 10-20parts of isoniazid, 500-1000 parts of sulfamic acid, 100-200 parts of a nutritional supplement, 0-200 parts of anhydrous magnesium sulfate and 10000 parts of a nutritional substrate. The preservativecan effectively delay the occurrence time of the maximum flower diameter of cut flowers through mutual cooperation of the components, prolong the ornamental time of the cut flowers, relieve chlorophyll degradation of the leaves of thecut flowers and prolong the vase life of the cut flowers. On the other hand, the invention discloses a cut flower preservative bottling liquid which is obtained by mixing and dissolving the cut flower preservative and water according to the mass-volume ratio of 1: 50-200kg / L.

The invention relates to a method for quickly preparing a water-soluble pigment by using enteromorpha. The method mainly comprises the following steps: performing pretreatment on raw material enteromorpha in a water phase; cleaning and performing filter pressing to obtain pretreated enteromorpha powder; performing acetone extraction and alkaline reaction to obtain an enteromorpha chlorophyll water-soluble pigment precipitant; removing impurities out of reaction supernatant by petroleum ether and performing reduced-pressure distillation to obtain an enteromorpha chlorophyll degradable product water-soluble pigment; mixing the pigment precipitants which are obtained in the two steps to obtain an enteromorpha water-soluble pigment finished product. Compared with the traditional chlorophyll water-soluble pigment preparation process, the method provided by the invention aims at instability and high degradation rate of enteromorpha chlorophyll, has the characteristics of easiness for operation, good economical performance, environment friendliness, high efficiency and quickness.

The invention relates to a new process of yellow tea and its preparation method; specifically, it is a kind of fresh leaves of tea trees with one bud and one leaf and one bud and two leaves as raw materials, which are processed according to the process of withering, killing green, rolling, stuffing yellow, drying, and improving fragrance. , a high-quality yellow tea processing method whose product has the quality characteristics of sweet (fresh) corn fragrance or rice cracker fragrance; the present invention increases the withering time, which can reduce the water content of fresh leaves; utilizes sun-dried yellow tea to create a high-temperature and high-humidity environment conditions, a large amount of chlorophyll is destroyed, so that the yellow substances such as theaflavins produced by non-enzymatic automatic oxidation of polyphenols under the action of heat and humidity are exposed, so as to achieve the purpose of improving the color quality of yellow tea; oxidize polyphenols and degrade chlorophyll ; The use of ultraviolet rays in the sun can inhibit the growth of undesirable microorganisms such as mold in the tea base, which is beneficial to the formation and safety of good quality yellow tea.

The invention relates to a method for quickly preparing a water-soluble pigment by using enteromorpha. The method mainly comprises the following steps: performing pretreatment on raw material enteromorpha in a water phase; cleaning and performing filter pressing to obtain pretreated enteromorpha powder; performing acetone extraction and alkaline reaction to obtain an enteromorpha chlorophyll water-soluble pigment precipitant; removing impurities out of reaction supernatant by petroleum ether and performing reduced-pressure distillation to obtain an enteromorpha chlorophyll degradable product water-soluble pigment; mixing the pigment precipitants which are obtained in the two steps to obtain an enteromorpha water-soluble pigment finished product. Compared with the traditional chlorophyll water-soluble pigment preparation process, the method provided by the invention aims at instability and high degradation rate of enteromorpha chlorophyll, has the characteristics of easiness for operation, good economical performance, environment friendliness, high efficiency and quickness.

The invention discloses a method for quickly preparing high-purity chlorophyll and chlorophyll degradation products. Satsuma mandarin peels and sweet potato leaves are used as raw materials for the first time to prepare the chlorophyll and derivatives thereof. The method disclosed by the invention is convenient and quick for taking materials, low in cost and easy to operate, and can complete the preparation of all the 8 products within three days. The purity of the obtained products can reach 90-95%, and the cost is much lower than the price of the same type of imported standard substance. The method can efficiently and quickly prepare the standard reference substances of the milligram-level chlorophyll and derivatives thereof required by the experiment, is suitable for popularization and application in commercial laboratories and enterprises, and has wide application prospects.

The invention discloses cymbidium sinense chlorophyll catabolism regulatory protein as well as a coding gene and application thereof. The amino acid sequence of the cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention comprises 307 amino acid residues and is shown in SEQ ID NO:2; the nucleotide sequence of a cymbidium sinense chlorophyll catabolism regulatory gene for coding the cymbidium sinense chlorophyll catabolism regulatory protein comprises 924 basic groups and is shown in SEQ ID NO:1. The cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention has the activity of chlorophyll degrading enzyme; after the expression quantity of the cymbidium sinense chlorophyll catabolism regulatory protein is increased, the content of chlorophyll in arabidopsis thaliana leaf blades can be decreased; after the cymbidium sinense chlorophyll catabolism regulatory protein is highly expressed in cymbidium sinense, the contents of chlorophyll in leaf blades and flowering branches of cymbidium sinense are obviously decreased. The cymbidium sinense chlorophyll catabolism regulatory gene and the cymbidium sinense chlorophyll catabolism regulatory protein coded by the same can be used for research on a chlorophyll degradation molecular mechanism and character improvement of leaf color or flower color of plants.

The invention discloses a refrigerating preservation method of fresh lotus seedpods. The method includes the following steps of 1, low-temperature cleaning of fresh-picked lotus seedpods; 2, low-temperature soaking and ozone water sterilization; 3, composite color-protecting preservative film formation treatment; 4, polypropylene preservation bag packaging, and conduction of low-temperature refrigeration at the temperature of 0-2 DEG C to obtain finished products. According to the method, microorganisms on the surfaces of the lotus seedpods can be effectively restrained or killed, photosynthesis, respiration, rising, glycometabolism and other physiological metabolism activities of the fresh lotus seedpods are retarded, and ethylene release and chlorophyll degradation can be restrained; color browning of the fresh lotus seedpods in the storage period is retarded, the appearance quality of the fresh lotus seedpods is fully reserved, and the nutritional quality and flavor of lotus seeds are well kept; the effective length for preservation of the fresh lotus seedpods can be kept for as long as 50 days through the preservation method. The method is simple and easy to carry out, high in operability, low in energy consumption cost and free of excessive investment, the process is environment-friendly, safe and free of pollution, the obtained products are practical and convenient to eat, the shelf life of the fresh lotus seedpods is greatly prolonged, and a new way is developed for high-value utilization of the lotus seed products.

The invention discloses a method for cultivating transgenic plants with enhanced plant root development and delayed leaf senescence. The gene shown in SEQ ID NO: 1 is introduced into the target plant and stably expressed and inherited to obtain root systems compared with non-transgenic control plants. Transgenic plants with enhanced development and delayed senescence. The transgenic plants can significantly promote the growth and development of the root system, and significantly enhance the photosynthesis of the functional leaves in the later stage of growth, delay the degradation of chlorophyll in the leaves, delay the senescence of the transgenic plants, and then increase the crop yield.