Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein and encoding gene thereof as well as application of encoding gene

A technology for chlorophyll degradation and related proteins, which is applied in the field of chlorophyll degradation and metabolic pathway related proteins and their coding genes and applications of cinnamon bamboo, chlorophyll degradation and metabolic pathway related proteins and their coding genes and applications, which can solve the problem of lack of genetic resources, etc. question

Inactive Publication Date: 2012-07-04
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are very few genetic resources available for bamboo plants.

Method used

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  • Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein and encoding gene thereof as well as application of encoding gene
  • Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein and encoding gene thereof as well as application of encoding gene
  • Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein and encoding gene thereof as well as application of encoding gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1, Chlorophyll Degradation and Metabolism Regulatory Related Protein Coding Genes BeCRN1 obtained.

[0022] 1.1 RNA extraction.

[0023] Take about 0.1g of the aged golden silk ci bamboo leaf material. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml TRIzol (Invitrogen Company), mix well, let stand at room temperature for 15 minutes, add 0.2ml chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, and then room temperature Leave for 5 minutes, centrifuge at 13000rpm, 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at room temperature for 15 minutes, centrifuge at 13000 rpm, 4°C for 15 minutes. The precipitate was washed with 70% ethanol and dried at room temperature for 15 minutes. Dissolve in an appropriate amount of ddH treated with 0.1% DEPC 2 O water, stored at -80°C for later use.

[0024] 1.2 cDNA first-strand synthesis and reverse transcription...

Embodiment 2

[0115] Example 2: BeCRN1 functional analysis.

[0116] 2.1 Sequence comparison analysis.

[0117] In order to analyze the relationship between BeCRN1 and CRN1 proteins of other species, the phylogenetic tree of BeCRN1 and NYE proteins of other species was established by using MEGA software. The results showed that BeCRN1 was closest to CRN1 in barley ( figure 1 -a). The homology of BeCRN1 with other species was analyzed by Genedoc software. The analysis results showed that BeCRN1 had great homology with CRN1 proteins of other species ( figure 1 -b). OsCRN1 (rice CRN1), SbCRN1 (sorghum CRN1), VvCRN1 (grape CRN1), AtCRN1 (Arabidopsis CRN1).

[0118] 2.2 BeCRN1 Gene function analysis.

[0119] 2.2.1 Complementary Arabidopsis crn1-1 Vector construction.

[0120] Using primers CRN1-S and CRN1-A as primers to amplify Arabidopsis BeCRN1 The coding frame was cloned into pMD19-T vector (Takara), after the sequence was correct. use KpnI (GGTACC) and PstI (CTGCAG) (...

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Abstract

The invention belongs to the technical field of plant gene engineering, and in particular relates to a Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein and an encoding gene thereof as well as an application of the encoding gene. The chlorophyll degradation related protein provided by the invention is derived from Bambusa emeiensis 'viridiflavus'; and the name of the protein is BeCRN1, and the amino acid sequence of the protein is as shown in SEQIDNO: 2. The nucleotide sequence of the encoding gene of the Bambusa emeiensis 'viridiflavus' chlorophyll degradation pathway related protein BeCRN1 is as shown in SEQIDNO: 1, or a polynucleotide sequence for encoding the SEQIDNO: 2 amino acid sequence. The gene disclosed by the invention can be used for research on a Bambusa emeiensis 'viridiflavus' chlorophyll degradation mechanism and is used for color character improvement of ornamental bamboo plants, including improvement of green characters of ornamental bamboo plants and the like.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a protein related to a chlorophyll degradation metabolic pathway and its coding gene and application, in particular to a protein related to a chlorophyll degradation metabolic pathway of cinnamon bamboo and its coding gene and application. Background technique [0002] Chlorophyll is the main component for capturing light in photosynthesis. Chlorophyll is degraded on a large scale during the later stages of plant growth and development, leaf senescence or fruit ripening. Chlorophyll degradation plays an important role in the recycling of protein nitrogen during leaf senescence (H?rtensteiner S, Annu Rev Plant Biol, 57: 55-77, 2006); meanwhile, chlorophyll degradation is a detoxification method for senescent mesophyll cells (Matile et al. Plant Physiology and Biochemistry, 27: 595-604, 1989; Matile et al. Plant Physiology, 112: 1403-1409, 1996). The l...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10A01H5/00
Inventor 丁雨龙魏强曹慧敏胡佩
Owner NANJING FORESTRY UNIV
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