A kind of bacterial strain and degradation method for degrading chlorophyll

A chlorophyll and strain technology is applied to the chlorophyll-degrading strains and the field of degradation, which can solve problems such as residues and product pollution, and achieve the effects of low cost, fast degradation speed and high degradation rate.

Active Publication Date: 2011-12-21
广西桂林鹏宇兄弟柑桔产业开发有限责任公司
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Problems solved by technology

Because it is an oxidation method, when the chlorophyll is oxidized, the active ingredients are also oxidized to varying degrees; in addition, hydrogen peroxide or sodium hypochlorite remains in the finished product, causing pollution to the product

Method used

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  • A kind of bacterial strain and degradation method for degrading chlorophyll
  • A kind of bacterial strain and degradation method for degrading chlorophyll
  • A kind of bacterial strain and degradation method for degrading chlorophyll

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Experimental program
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Embodiment

[0024] One, the preparation method of Aspergillus GLHZB319 bacterial strain is:

[0025] Take 1.00g of soil with fallen leaves, add it to a triangular flask filled with 25mL of sterile water, shake it for 1min, and make the soil evenly dispersed to obtain a soil suspension; absorb 4 drops of the soil suspension, and drop it on the single chlorophyll extracted with 80% acetone On the plate medium prepared with agar, spread evenly with a coating ring, and check the plate culture results after 3 days of constant temperature cultivation at 30°C; the strains that can grow on the plate and produce transparent circles will be extracted by a single strain extracted in 80% acetone The fermentation effect in the chlorophyll aqueous solution was further confirmed. The fermentation conditions were: temperature 30°C, natural pH, rotation speed 100r / min. Aspergillus GLHZB319 strain had the best fermentation effect.

[0026] Characteristics of Aspergillus GLHZB319 strain: The colonies on pot...

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Abstract

The invention discloses a strain and method for degrading chlorophyll. The name of the strain determined by China Center for Type Culture Collection is Aspergillus GLHZB319, and the collection number is CCTCC NO:M2011101. The residual chlorophyll on the plant is degraded by a microbial fermentation method. When the strain ferments and degrades chlorophyll, no additional carbon source, nitrogen source or inorganic sodium salt is needed; and in the strain growth and development process, the chlorophyll can be used as a substrate for degrading chlorophyll. The strain has the characteristics of high degradation speed, high degradation rate, low cost, no residue, no pollution, high safety and environmental protection for chlorophyll, and can partially substitute decolorizing resin in the existing natural product extraction process.

Description

technical field [0001] The invention relates to the fields of life science, biotechnology, bioengineering and biochemical industry, in particular to a bacterial strain and a degradation method for degrading chlorophyll. Background technique [0002] Chlorophyll exists on the membrane of the chloroplast of plant leaves and is the place where green plants carry out photosynthesis, including chlorophyll a and chlorophyll b. The molecular structure of chlorophyll is composed of leaf alcohol group and magnesium porphyrin, which is composed of a magnesium ion and nitrogen on four pyrrole rings. In addition, the chemical structure of magnesium porphyrin in plant chlorophyll is very similar to that of iron porphyrin in animal red blood cells, and appropriate supplementation of chlorophyll is very helpful for the recovery of hematopoietic function in patients with accidental blood loss. [0003] In contemporary society, a considerable part of industrial raw materials, food, health p...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/66
Inventor 郝再彬陈志良王彦超李海云张会香
Owner 广西桂林鹏宇兄弟柑桔产业开发有限责任公司
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