Method for improving cold resistance of red bract pineapples

A technology of cold resistance and pineapple, which is applied in the field of improving the cold resistance of red bud pineapple, can solve the problems of cold resistance of red bud pineapple plants without good cultivation measures, and achieve the effects of promoting photosynthesis, reducing damage, and alleviating degradation

Pending Publication Date: 2021-07-09
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solving the low temperature damage of red bud pineapple plants has always been an important issue in production, and has attracted the attention of scientific workers. However, there are no good cultivation measures to improve the cold resistance of red bud pineapple plants and realize their growth in winter. Outdoor cultivation in cooler regions

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  • Method for improving cold resistance of red bract pineapples
  • Method for improving cold resistance of red bract pineapples
  • Method for improving cold resistance of red bract pineapples

Examples

Experimental program
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Effect test

Embodiment 1

[0070] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:

[0071] Step 1, add the tissue-cultured seedlings of red bud pineapple with a height of 3-4cm, 3-5 roots and 5-8 leaves to MS medium containing ABA for cultivation, and the control parameters of the cultivation process are: ABA is cultivated in the MS medium The final concentration in the medium was 8mg / L, the culture temperature was 25±2°C, the illuminance was 15001x, the humidity was 80%, and the culture time was 30 days.

[0072] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.

[0073] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the...

Embodiment 2

[0077] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:

[0078] Step 1, add the tissue-cultured red bud pineapple seedlings with a height of 3 to 4 cm, 3-5 roots and 5-8 leaves to MS medium containing SA for cultivation, and the control parameters of the cultivation process are: SA is cultivated in the MS The final concentration in the medium is 5 mg / L, the culture temperature is 25±2° C., the light intensity is 1500 lx, the humidity is 80%, and the culture time is 20 days.

[0079] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.

[0080] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the cond...

Embodiment 3

[0084] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:

[0085] Step 1, the red bract pineapple tissue culture seedling that height is 3~4cm, has 3-5 roots and 5-8 leaves is moved into containing CaCl 2 MS medium culture, the control parameters of the culture process are: CaCl 2 The final concentration in the MS medium is 5 mg / L, the culture temperature is 25±2° C., the illuminance is 1500 lx, the humidity is 80%, and the culture time is 40 days.

[0086] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.

[0087] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the conductivity of the tissue extr...

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Abstract

The invention provides a method for improving cold resistance of red bract pineapples, and belongs to the field of plant cultivation. The method for improving cold resistance of the red bract pineapples comprises the following steps that S1, tissue culture seedlings of the red bract pineapples are inoculated into an MS culture medium containing a low-temperature treating agent for culture; and S2, low-temperature exercise treatment is conducted after culture is completed. According to the method for improving cold resistance of the red bract pineapples, the activity of protective enzyme systems (SOD, POD and CAT in bodies of the red bract pineapples can be improved, so that the damage of low-temperature stress to the red bract pineapples is reduced, the integrity of leaf structures is kept, chlorophyll degradation is relieved, photosynthesis is promoted, and the overall cold resistance of the red bract pineapples is improved.

Description

technical field [0001] The invention belongs to the technical field of ornamental flower cultivation, and in particular relates to a method for improving the cold resistance of red bud pineapple. Background technique [0002] Red bud pineapple, also known as Phnom Penh pineapple and Yan pineapple, is a perennial terrestrial evergreen herb of the family Bromeliaceae. The red bud pineapple has a long flowering period, the plant is rough and peculiar, full of wild charm, and the fruit shape is like a pineapple and it can be appreciated for a long time, so it is also known as "pineapple flower". The red bud pineapple is native to Argentina. It likes a warm and humid environment and well-ventilated living conditions. It likes sunlight and can tolerate drought. The red bud pineapple has certain requirements for the sunshine in the place where it grows. normal growth and development. If the temperature is low, red bud pineapples are prone to chilling damage, which will cause the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/60
CPCA01G22/60
Inventor 马均何业华钟小兰薛彦斌周徐子鑫毛美琴杨威
Owner SICHUAN AGRI UNIV
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