Key gene for controlling chlorophyll metabolism and method for establishing plant green residence character therewith

Abstract

The invention uses fast neutron mutagenesis mode plant south-leaf mustard to filter to gain stay-green mutant. Separating crucial adjusting gene AtNYE1 would be gained by the method of image-location clone. And the stay-green feature would be created by RNA interrupt or other technology, and through NYE1 gene or the expression to restrain the gene, and lower the albumen activity of NYE1.

Description

technical field [0001] The invention relates to a novel gene AtNYE1 which regulates chlorophyll degradation metabolism and causes leaves to turn yellow in the normal aging process of plants. Use RNAi technology to inhibit the expression of the gene, or induce the mutation of the gene through fast neutrons to reduce or inactivate the activity of the protein, so as to obtain the plant traits of staying green. Background technique [0002] After the plant leaves enter the senescence process, the yellowing phenomenon caused by the rapid degradation of chlorophyll is the common and most notable appearance characteristic of almost all plant leaves senescence. Although leaf senescence can be induced by many environmental and developmental factors, the timing and process of leaf senescence are regulated by apoptosis. In the past few decades, scholars have found that the leaf green period is delayed until maturity or senescence in many crops, that is, stay-green (stay-green) or non-...

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Problems solved by technology

[0008] So far, although the physiological and morphological characteristics of several natural mutations or artificially induced mutants have been reported, the key genes that significantly regulate the kinetics of chlorophyll degradation in aging leaves have not been isolated yet.

Among the three key enzyme genes in the chlorophyll degradation metabolic pathway that have been cloned (chlorophyllase gene, pheophorbide oxidase gene, RCC gene), only pheophorbide oxidase gene showed a certain degree of regulation of aging leaves potential for chlorophyll degradation, but mutation and over-repression of this gene can cause accumulation of toxic intermediates, leading to necrotic spots on leaves similar to those caused by trauma (Hrtensteiner, S.Annu.Rev.Plant Biol.57 :55-77, 2006)

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