47 results about "Cymbidium sinense" patented technology

A culture method of cymbidium hybrid orchids is characterized by comprising steps of greenhouse culture and blooming regulation of parent cymbidium hybrid, hybrid orchids and cymbidium materials: a) culture of a female parent material; b) culture of a male parent material; c) hybrid pollination; d) ovary and seed development; e) sterile sowing and the like. The method is used for culturing good hybrid orchid offspring materials with characteristics of cymbidium; different from existing cymbidium hybrid and other cymbidium sinense hybrid orchids, cymbidium ensifolium hybrid orchids and the like in the aspect of morphological characteristics, growth characteristics, stress resistance and the like, the cymbidium hybrid orchids have excellent properties of the cymbidium, and the variety type of the cymbidium hybrid orchids can be enriched.

The invention discloses a method for regulating and controlling the flowering stage and flowering traits of cymbidium sinense. According to the method, through fertilizer and water, illumination and temperature management in the flower forcing process, a plant growth regulator mixed solution I is sprayed in the early stage of flower bud differentiation to promote flower bud differentiation to be completed in advance, and a plant growth regulator mixed solution II is sprayed again in the flower arrow elongation stage to promote flower arrow elongation and inflorescence development. By means ofthe method, the number of buds of each pot is 2-3, the number of flowers on each inflorescence is increased and is averagely three more than that of contrast, the flowers become larger, and the initial flowering period can be advanced by 24 days. According to the method, the cymbidium sinense is stabilized to bloom before the Spring Festival, the blooming quality is improved, the arrow is increased and lengthened, the inflorescence is lengthened, the number of flowers is increased, the flowers are enlarged, the ornamental effect is better, a foundation is laid for meeting the huge demand of phalaenopsis orchid, the purposes of further expanding the market and increasing the market share of the cymbidium sinense are achieved, and economic benefits are outstanding.

The invention discloses a Streptomyces castanea strain CPAT-W03 and application thereof. The Streptomyces castanea strain 2 is classified and named as Streptomyces badius, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 13, 2020, the preservation number is 19342, and the Streptomyces castanea belongs to the field of bacteria, actinomycetes phylum, actinomycetes class, streptomycete orders, streptomycete families and Streptomyces sp. The application is the application of the Streptomyces castanea strain 2 in biological prevention and control of stem rot of Cymbidium sinense.

The invention discloses bacterium paenibacillus polymyma SHL-1 and an application thereof in preventing and controlling stalk rot of cymbidium sinense. The bacterium paenibacillus polymyma SHL-1 with a preservation number of CCTCCM2011441 is preserved in the China Center for Type Culture Collection on December 2, 2011. In the invention, the bacterium paenibacillus polymyma SHL-1 is inoculated to an LB (Langmuir-Blodgett) liquid culture medium with pH of 7.0, and is cultured for 48 hours in a oscillation way at 200r / min at a temperature of 28 DEG C, so that a biological preparation can be prepared. The biological preparation has a strong inhibition effect to the stalk rot of cymbidium sinense, and has environmentally-friendly non-toxic sources and small influence to biological environment;and furthermore, the biological preparation has low requirements to the culture condition, has an inhibition effect to various pathogenic fungi, and has good development and application prospects.

The invention discloses cymbidium sinense chlorophyll catabolism regulatory protein as well as a coding gene and application thereof. The amino acid sequence of the cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention comprises 307 amino acid residues and is shown in SEQ ID NO:2; the nucleotide sequence of a cymbidium sinense chlorophyll catabolism regulatory gene for coding the cymbidium sinense chlorophyll catabolism regulatory protein comprises 924 basic groups and is shown in SEQ ID NO:1. The cymbidium sinense chlorophyll catabolism regulatory protein disclosed by the invention has the activity of chlorophyll degrading enzyme; after the expression quantity of the cymbidium sinense chlorophyll catabolism regulatory protein is increased, the content of chlorophyll in arabidopsis thaliana leaf blades can be decreased; after the cymbidium sinense chlorophyll catabolism regulatory protein is highly expressed in cymbidium sinense, the contents of chlorophyll in leaf blades and flowering branches of cymbidium sinense are obviously decreased. The cymbidium sinense chlorophyll catabolism regulatory gene and the cymbidium sinense chlorophyll catabolism regulatory protein coded by the same can be used for research on a chlorophyll degradation molecular mechanism and character improvement of leaf color or flower color of plants.