89 results about "Endangered species" patented technology

The invention relates to a genetic map construction and haplotype analytical method based on single-cell whole genome sequencing, and a device used for the method. The genetic map construction method comprises the following steps: comparing a whole genome sequence of a single cell of a species with a reference sequence to obtain a statistic result of a single nucleotide polymorphic site genotype; performing parent type classification of the genotype result to obtain a most appropriate male parent / female parent (a / b) result, so as to obtain a minimum recombination frequency of all the cells in the statistic result; according to the type classification result, dividing a linkage area (bin); calculating a change ratio of a / b to obtain recombination rates between all adjacent bins; and finally obtaining the genetic map according to the recombination rate of each recombinant site. The method of the invention can obtain a genetic map with high resolution by using a single cell, can not only applicable to human, but also applicable to some higher organisms with limited fertility, especially to endangered species.

The invention relates to a method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which comprises the following steps: preferably selecting, breeding and feeding parent fishes; regulating and controlling water temperature, illumination and water flow to enable the parent fishes to grow; and enhancing the nutrition to promote gonad mature ovulation. The technical method is used for screening artificial ripening effective temperature, intensity of illumination and water flow velocity factor data, screening varieties and doses of artificial ripening effective nutrition enhancers, screening the time suitable for gametogenesis and fertilization of cheilinus undulates, and acquiring high-quality fertilized eggs of cheilinus undulates. The fertilization rate of the eggs of the cheilinus undulates acquired by the method is 20-86%, and the hatching rate is 30-78%. The technical method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates, which is provided by the invention, has the characteristics of advancement, high efficiency, practicality and reliability and comprehensively regulates and controls temperature, illumination, water flow and nutrition enhancement in the breeding process of seedlings of cheilinus undulates. The invention has great application value and wide popularization and application prospects for protecting endangered species.

The invention provides a method for breeding and cultivating taxus mairei with a seed, and the method comprises the following steps that a taxus mairei seedling is obtained by breeding and cultivating the sexual seed; the taxus mairei seedling is transplanted and planted, and a large taxus mairei seedling and a young tree are obtained; and the cultivation area of the taxus mairei is expanded. Special cultivation management measures for the taxus mairei are adopted to establish a taxus mairei breeding and cultivation technical system, a manual way to breed the taxus mairei is opened up, so that the large-scale industrial production of the taxus mairei becomes possible, the market demand for the taxus mairei is met, and a good test system is provided for protecting endangered species of wild plant resources.

The invention discloses a method for culturing seedlings of Phoebe bournei by a light soil type mixed matrix, which comprises the procedures of collecting seeds, sowing seeds, preparing the matrix, transplanting and culturing seedlings. The method comprises the following steps of: sowing seeds from strong and good seed trees which are more than 20 years old into a sand bed; transplanting seedlings into a plastic cup filled with the light soil type mixed matrix including 30-40 parts of yellow soil, 40-50 parts of coconut husk immersed dry material, 14.75-24.5 parts of pond sludge and 0.25-0.75parts of calcium magnesium phosphate fertilizer; cultivating the seeds to obtain good and strong Phoebe bournei seedlings with the height of more than 31-40 cm; and transplanting the seedlings to carry out the forestation. The seedling culturing matrix of the method provided by the invention has good water conversation performance, good fertilizer conversation performance and complete nutrition, the surface is not easy to harden and fewer weeds are existed; the cultivated seedlings are thickset, the growth is fast, the time for culturing seedlings is shortened, the survival rate of the forestation is high and the recovering stage of the forestation is short, so that the method is adapted to the requirement of accelerating a planted forest of the rare and endangered species Phoebe bournei,and has better economic benefits and social benefits.

The invention relates to an ovarian large cortex piece vitrified cryopreservation protection liquid and a cryopreservation method thereof. Although the vitrified cryopreservation method is widely applied to experimental animals, a universal cryopreservation method and a standard cryopreservation liquid preparation scheme are absent at present, so that the sized transplantation subjected to vitrified cryopreservation is at a small-size stage, an ovarian small cortex piece has limited follicles, and the service life of the transplanted follicles is influenced. The invention provides vitrified cryopreservation protection liquid suitable for an ovarian large cortex piece, and gonadotropin is added on the basis of the common vitrified liquid. According to the vitrified cryopreservation protection liquid suitable for the ovarian large cortex piece, especially the ovaries of experimental animals, improved variety livestock and wild endangered species in sudden death are conveniently cryopreserved at the most proper osmotic equilibrium time of performing vitrified cryopreservation on the ovarian large cortex pieces of different sizes; and moreover, according to the method, the survival rate of the follicles can be improved, the blood reperfusion time of the transplant is shortened, and the survival rate of the cryopreserved ovarian transplantation in vivo is improved.

The invention provides a quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method which relates to a quick and high-efficiency plant seed germinating and high-quality seedling breeding method. The method comprises the following steps: (1) seed collection: collecting cephalotaxus hainanensis seeds to be matured or matured seeds; (2) preparation of seed germinating matrixes; (3) release of seed dormancy; (4) seed germination; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud ratio by more than 85%,the seedling viability exceeds 90%, the uniform high-quality cephalotaxus hainanensi seedlings can be produced, and the viability of seeds transplanted to fields can also exceed 90%; compared with the traditional method, the stocking time is shortened by about 2 / 3; the related facilities and processes of tissue culture and the like are not required, so the operation is convenient; the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources.

A method and system for efficient and eco-friendly shrimp skimmer trawling by a trawling vessel with a trawl net, skimmer booms, net arms, and buoyantly adjustable rollers to maintain trawl net height and net opening without damage to the ocean floor allowing for systematic and frequent retrieval of catch while continuing trawling operations in intervals short enough to prevent death of by-catch and endangered species and where the system is fully storable on trailerable trawling vessels for maintenance of allowable highway heights. The system and method has the upper edge of the trawl net held above the ocean surface while the trawling vessel moves forward.

The acer yangbiense is regarded as one of the rarest or endangered species in the world, so a nursing method thereof and a population quantity enlarging method thereof are the basic methods for saving the species. The selected plants of the acer yangbiense which are cultured by the invention are researched in the following aspects of conservation biology, environmental biology, and the like, to provide material foundation, scientific basis and technical support for leading the acer yangbiense back to nature and realizing species reconstruction. The invention comprises the steps of breaking the dormancy of the acer yangbiense seedlings; firstly, sterilizing with 0.05% potassium permanganate solution for 10 minutes; secondly, putting the seedlings under the environment of 5 DEG.C after seed dressing with 80% Zineb wettable powder; putting the seedlings under the environment of 15 DEG.C-20 DEG.C for eight hours twice a moth; germinating; processing for 4 months; and seeding into a growing media with turf: perlite: peat: red earth: organic fertilizer=1:1:1:1:1 after the seedlings germinate.

The invention provides a quick and high-efficiency cephalotaxus hainanensis seed seedling breeding method which belongs to a quick and high-efficiency plant seed seedling breeding method. The method comprises the following steps: (1) seed collection; (2) cleaning and sterilization of seeds; (3) release of seed dormancy at low temperature; (4) sterile germination of seeds; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud rate to more than 90% (enhanced by about 70%), and the seeding viability exceeds 90% (enhanced by about 60%); compared with the traditional method, the stocking time is shortened by about 2 / 3; the regular high-quality cephalotaxus breeding seedlings can be produced; and the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and utilization of resources.

Paphiopedilum armeniacum, known as 'Golden Child', is one of orchidaceous endangered species in China and has extremely high aesthetic values. Successful and perfect artificial propagation technology has significant importance to the protection and development of paphiopedilum armeniacum. The invention discloses a method which is to introduce tulasnella c alospora into a substrate by using a grown paphiopedilum armeniacum plant and then cultivate aseptic seedlings of paphiopedilum armeniacum in the substrate carrying tulasnella c alospora so as to raise mycorrhizal seedlings of paphiopedilum armeniacum. The invention aims to provide a method for raising mycorrhizal seedlings of paphiopedilum armeniacum with low cost and easy operation to solve the problem of slow growth of cultivated aseptic seedlings. The method has the advantages of readily available tulasnella c alospora resource, easy operation of impregnation, low cost, etc., and has obvious economic and ecological efficiency.

The extracorporeal culture and mature method of immature oocyte from ovary tissue block includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell, compounding MSC culture liquid and agar and covering the culture rack, adding HCG in 3 IU / ml, and adhering the ovary tissue block on the rack to culture. In the co-culture system, estradiol, progestin and luteotopin contents are increased obviously, and after culturing, there is mature oocyte discharged. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to a valeriana jatamansi jones breeding method, which is characterized in that the field culture transplanting is carried out after tissue culture breeding or seed breeding; the tissue culture breeding seedling comprises measures including sterile system establishing, culture medium adoption as callus culture, propagation culture and rooting culture, exercising seedling and acclimation seedling treatment, and seedling bed and young seedling disease prevention after the planting; and the seed breeding seedling comprises measures including seed collection time, dormancy stage breaking, bud cell differentiation induction and seedling period disease and pest prevention. The method provided by the invention mainly solves the problem of valeriana jatamansi jones breeding industrialization, the reliable guarantee is provided for the planting, the endangered species are effectively protected, and particularly, the obvious progress is realized in the field of endangered medicinal material rescue.

The invention relates to a method for breeding cultivation seedlings of rare or endangered euryodendron excelsum artificially. The method comprises the following steps of: collecting mature euryodendron excelsum fruits in a species distribution area, and cleaning mature seeds; preparing a sprouting medium by using humus soil and loamy soil in a volume ratio of (1-3):1; mixing the seeds and dried river sand uniformly, broadcasting the mixture uniformly on a sprouting medium, covering a layer of surface humus soil, keeping the temperature of between 20 and 25 DEG C for 4 to 6 weeks to obtain seedlings with true leaves; after the seeds germinate, transplanting the seedlings with 2 to 5 true leaves, wherein the seedling transplanting medium is the same as the sprouting medium; and after transplanting the seedlings, ensuring moisture required by the growth in the seedling stage, allowing the seedlings in the environment of high temperature and high humidity to grow, and growing for 18 to 24 weeks to perform field transplantation. By the method, the seed germination rate of the euryodendron excelsum is over 70 percent, the survival rate of the seedlings is over 80 percent, the cultivation seedlings are robust, and 'little old seedlings' are avoided. The method is suitable for large-scale field transplantation. The method provides a technical guarantee for the salvation of the rare or endangered species, the recovery of population and the effective protection of resources.

The invention discloses a seeding and propagating method of a lotus leaf adiantum spore, and belongs to the technical field of forest propagation. The main technical point is to use lotus leaf adiantum spore as a material, apply a series of measures such as selection and disinfection of seeding matrix, disinfection of sowing container and drug and cultivation environment of seeding and emergence periods, and moisture management, so as to promote the spore seeding and propagating efficiency. The seeding and propagating method of the lotus leaf adiantum spore is simple in method, short in seedling time, high in seedling rate, high in survival rate of transplanting, and can reduce the production cost, and is significant to protect national rare and endangered species.

The invention discloses a leaf micro-cuttage propagation technology for the rare and endangered plant Begonia coptidifolia. According to the invention, innovation on the size of a cutting is realized, a propagation coefficient is greatly improved, and the technology is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia; the technology can be carried out in an appropriate temperature range of 20 to 30 DEG C; perlite, vermiculite or other matrixes are used for leaf micro-cuttage propagation of the rare and endangered plant Begonia coptidifolia in China; and a cutting with a size of as small as 0.5 cm can undergo normal differentiation and development and grow into a seedling. The technology makes a breakthrough to the conventional concept about the size limit of cuttings, greatly improves the propagation coefficient, is improvement and innovation of asexual reproductive technology and is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia. The technology is also applicable to begonia and other plant species suitable for leaf cuttage propagation.

The method of obtaining immature oocyte from ovary tissue through separating or extracting for extracorporeal culture includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell or culturing the immature oocyte in the MSC culture liquid. After culturing, the co-culture system has obviously increased estradiol, progestin and luteotopin contents, and over 90 % of the immature oocyte become mature. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention discloses a method for simulating potential distribution of endangered pinaceae plants. The method comprises the following steps: firstly obtaining actual distribution points and distribution ranges of an endangered species, selecting main environment variables for the distribution of the species so as to construct an actual appearance database; on such basis, constructing a virtual distribution database, randomly drawing part of data from the virtual distribution database as a modeling database, and taking the rest part as a verification database; on the basis of the modeling database, selecting a plurality of ecological niche models to construct a plurality of prediction databases; comparing a model prediction database with the verification database, and selecting optimized combination of geographic position accuracy, environment variable models, the ecological niche models and methods; on the basis of the selected optimized combination and the actual appearance database, finally constructing an endangered species potential distribution database. The method disclosed in the invention can be used for overcoming the defects of bad accuracy, large error and high uncertainty factors of the endangered species simulation, and is beneficial for greatly improving the prediction effect.

The invention discloses a Cinnamonum kanehirae Hayata somatic embryo culture medium, which contains MS as a basic culture medium or MS salts and MT vitamin, and also contains growth regulators such as BA, Picloram, ABA and NAA, 170mg / L sodium dihydrogen phosphate monohydrate,30mg / L adenine sulfate, 0.5g / L caseinhydrolysate, 1.2 percent agaragar, and 3 percent saccharose, wherein three culture media for somatic embryogenesis, somatic embryo proliferation and embryo differentiation, and embryo rooting are prepared respectively. The tissue culture and rapid propagation method of Cinnamonum kanehirae Hayata by using the culture media comprises the following four steps: 1. the acquisition and disinfection of an explant; 2. the induction of a somatic embryo; 3. the somatic embryo proliferation and embryo differentiation; and 4. root induction and transplanting. The invention initially creates a complete set of method for Cinnamonum kanehirae Hayata somatic embryos to reproduce seedlings, saves the endangered species resource, lays a foundation for further exploring the gene engineering of somatic embryos, broadening explant materials and so on, and provides adequate wood for the mass production of Antrodia camphorata with extremely high economic value.

The invention discloses an artificial purification and rejuvenation planting method for introduction of angustifoliate Stachys baicalensis Tisch ex Benth. Through identification of different varieties in the wild, the angustifoliate Stachys baicalensis Tisch ex Benth having a good phenotype and meeting the requirements of nectariferous plants are introduced and cultivated, and purification and rejuvenation are conducted in a nursery field by means of a special planting method; through phenotypic selection (such as large stem diameter, firmness and uprightness, lodging resistance and the like), seeds of good plants are picked, and seeds which meet demands after secondary selection are stored for reproduction, so that seed sources are provided for large-scale artificial planting of the angustifoliate Stachys baicalensis Tisch ex Benth. Not only is the endangered species, the angustifoliate Stachys baicalensis Tisch ex Benth saved, but more importantly, the optimal species of ecological and economical double orientation and co-fusion is provided for the national restoration project of the three river wetland reserve through artificial reproduction.

The present invention is feeder cell for extracorporeal culture of immature oocyte and features that marrow mesenchyme stem cell MSC of female mouse is used as the feeder cell. The feeder cell is easy to obtain, can secrete several kinds of cell factors and growth factors, promote over 90 % of the immature oocyte to become mature and promote the growth, ovulation and maturing of the ovarian follicles in the ovary tissue block under extracorporeal culture. The present invention is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention discloses a green globular body tissue culture propagation method for Alsophila costularis. The method includes the steps of obtaining aseptic explants and inducing green globular bodies. In the inducing process, culture is performed through two inducing pre-culture media, then culture is performed through a green globular body inducing culture medium, and the green globular bodies are obtained; then, alternate culture is performed through two proliferation culture media, differentiation culture and rooting culture are performed, and tissue culture seedlings are obtained. Through the method, the dendritic woody fern green globular bodies are successfully induced, the propagation efficiency is high, the green globular body inducing rate reaches 82%-88%, the proliferation multiple reaches 7.5-9.3, the differentiation rate of the green globoids reaches 100%, the rooting rate reaches 100%, and the propagation period is short; the method has great significance in increasing the population quantity of the rare endangered fern Alsophila costularis and relieving the endangered situation of the rare endangered fern Alsophila costularis, and meanwhile in-vitro preservation of germplasm resources of endangered species is achieved.

The invention discloses an artificial breeding method for rhododendron huadingense seedlings and belongs to the technical field of rare plant artificial breeding.The method comprises the following steps of fruit collection, seed preservation, seed germination, seedling hardening for the first time, seedling hardening for the second time and seedling transplanting.By means of the artificial breeding method, the germination rate of rhododendron huadingense seeds can be high and exceed 40%, the survival rate of the seedlings is high, the total survival rate can reach 75%, the seedlings which is successfully bred grow well, it is beneficial to protect the rare or endangered species rhododendron huadingense, and it is also beneficial to develop and utilize the plant as an ornamental flower variety.

The invention discloses a DNA bar code primer for quickly identifying Budorcas taxicolor and also discloses a molecular biological method for quickly identifying Budorcas taxicolor. Total DNA of Budorcas taxicolor, Bos grunniens and Bos taurus domestica are extracted by using a standard phenol-chloroform method or a DNA extraction kit; mitochondrial Cytb sequences of Budorcas taxicolor, Bos grunniens and Bos taurus domestica are subjected to PCR (polymerase chain reaction) amplification and sequencing by using the DNA bar code primer to obtain markers; species identification is carried out according to sequence-specific sites of nucleotide of Budorcas taxicolor. Cytb gene sequences of mitochondrial DNA of Budorcas taxicolor are measured through DNA bar codes, namely amplification for the first time, thus identifying the three species; necessary molecular biological evidence is provided for effective protection of endangered species and effective struggle against illegal hunters.

The invention provides a cephalotaxus hainanensis embryo culturing and seedling breeding method which relates to a plant quick propagating and breeding method. The method comprises the following steps: (1) collection and selection of explants; (2) cleaning and sterilization of seeds and embryo obtainment; (3) inoculation and culture of embryos; (4) passage and root induction of test-tube seedlings; and (6) acclimatization and transplantation. The invention firstly creates the whole method of using cephalotaxus hainanensis embryos as explants to perform tissue culture and quick propagation, andprovides high-quality culture mediums for breeding the cephalotaxus hainanensis embryos, thereby not only producing regular high-quality cephalotaxus hainanensis seedlings and providing a technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources, but also laying foundation for further exploring gene engineering of cephalotaxus hainanensis, widening explant materials, and the like.

The invention discloses a method for sampling and extracting DNA from living echinoderms. The tube podia of living sea urchins or tube podia, tentacles or intestines of living sea cucumbers are taken, lysed and digested with a lysate added with DNase, and extracted and centrifuged to obtain the DNA. Supernatant, centrifugal removal of slag (bone flakes), ethanol precipitation, centrifugal separation, detection and other steps make the concentration and quality of the extracted DNA consistent with the DNA extracted from the traditional (dead body) sampling tissue of echinoderms. It is not necessary to kill echinoderms, but samples are taken from living organisms, and then DNA is extracted, so that molecular biology techniques can truly become an auxiliary tool for genetic breeding. Utilizing the invention, the echinoderm resources can be rationally utilized to carry out auxiliary breeding, breeding status monitoring, gene screening, family identification and the like. In addition, it is of great significance to the protection and research of many endangered species of echinoderms.

The invention relates to the dominance evaluation of ecological communities at the community level and species level, and mainly invents a concept and a method capable of comprehensively evaluating the dominance at the community level and species level. The concept and the method are used to monitor endangered species in macro-ecological communities and to diagnose and predict the risk of human flora-related diseases in microbial communities. The inventor defines a dominance index at the community level, a species dominance distance at the species level and a species dominance index. The threeindexes are defined by a unified mathematical concept and method, and can be used to evaluate the dominance of various biological / ecological communities (including macro-ecological communities (suchas tropical rainforests) and human microbial communities) in various time and / or space scales. In the specification, the microbial flora (community) in the female reproductive tract is taken as an example, and the dominance index is used to study the dominance change of the community in the time scale and the dominance of each species in the community and identify the difference in dominance of the same species in different communities (in the space scale).

The invention relates to a method of establishing a novel induction system to reprogram CEFs (chicken embryo fibroblasts) into PGCs (primordial germ cells) and belongs to the technical field of biology. In isolated primordial CEF, OCT4, SOX2, NANOG and LIN28 reprogramming factors are introduced to lentivirus exogenously; 21 days after continuous culture, iPSC (induced pluripotent stem cells) strain identification is performed with an SSEA-1 antibody; a positive cell line that is identified is replaced with a BMP4 / BMP8b / EGF induction system; iPGCs (induced primordial germ cells) are acquired byinduction. The problem is solved herein that the quantity of PGCs acquired in endangered species is low; germplasms resource protection and species restoration are achieved for the engendered chickenspecies.

The invention relates to a calculating method for the ecological water demand of plecoglossus altivelis in the fattening period. The calculating method comprises steps as follows: analyzing functionalcharacteristics of fattening farm habitats of the plecoglossus altivelis in the fattening period; analyzing characteristics of periphytic algae; analyzing factors affecting baits of the plecoglossusaltivelis; calculating water velocity and water demand in non-flood period; calculating water velocity and water demand of a fattening farm of the plecoglossus altivelis in the flood period. The conventional calculation for the ecological water demand is short of analysis for problems about the environment required by actual survival and growth of organisms. According to the method, the plecoglossus altivelis which is an endangered species is selected as an ecological protection objective, the functional characteristics of the fattening farm habitats of the plecoglossus altivelis in the fattening period are analyzed firstly, then flow conditions required by the habitat functions are further analyzed, and finally, the calculating method for the proper ecological water demand required by survival and growth of the plecoglossus altivelis in the fattening period is proposed. With the calculation of the ecological water demand, habitat environments and functions of endangered fishes are repaired and improved, the calculation and evaluation method of the ecological water demand is enriched and developed, and scientific basis is provided for biodiversity protection and water resource scheduling management.

The invention discloses a method for grafting semiliquidambar cathayensis. The method comprises the following steps: selecting a maple tree without diseases as a tree stock and selecting a semiliquidambar cathayensis branch with the diameter approximate to that of the tree stock as a cladus; intercepting 1-2cm of the cladus to serve as a scion, wherein the section is vertical to the length direction of the branch; cutting the tree stock at a to-be-grafted part, wherein the section is vertical to the height direction of the tree stock; tightly attaching the scion section to the tree stock section and aligning cambium layers; tying and fixing graft union. The method is simple and practical; the graft union is easy to cut; the wound surface and graft union surface of grafting are smallest; the cambium layers are aligned, so that the survival rate is up to 93%, and new plant grows rapidly; the scion utilization rate is high; the method is beneficial to preservation and reproduction of rare endangered species.

The invention discloses a method for desertification control through salix bangongensis cuttage in a high-altitude gobi wind-blown sand region. The method includes the step that the groove cuttage salix bangongensis deep cuttage technology and the sand control and afforestation technology through salix bangongensis cuttage in the secondary desertification wind-blown sand region with the combination of protection of a surrounding gravel high-bank sandy barrier and recovery and protection of a platform biological living sandy barrier are formed by easily preparing sand in the secondary desertification wind-blown sand region. Measures are adjusted according to local conditions, local materials are used, engineering measures and biological measures are combined, and the method for desertification control through salix bangongensis cuttage in the high-altitude, high-coldness and high-drought gobi wind-blown sand region is easy to implement, economical, high in operability and beneficial to protection, expansion, utilization and popularization of endangered species.