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Molecular biological method for quickly identifying Budorcas taxicolor

A technology of molecular biology and species identification, which is applied in the field of molecular biology for rapid identification of takins, can solve the problems of distinguishing and identifying takins, and achieve the effect of saving time and reducing costs

Inactive Publication Date: 2016-02-24
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of DNA barcodes as molecular markers to identify takin, yellow cattle and yaks at home and abroad.

Method used

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  • Molecular biological method for quickly identifying Budorcas taxicolor
  • Molecular biological method for quickly identifying Budorcas taxicolor
  • Molecular biological method for quickly identifying Budorcas taxicolor

Examples

Experimental program
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Embodiment 1

[0024] 1. Use phenol-chloroform method or DNA extraction kit to extract the total DNA of takin, yak and cattle. In order to avoid contamination between animal residues and humans, the superficial muscle was removed, and DNA was extracted from fresh, uncontaminated muscle. DNA extraction refers to the standard phenol-chloroform method (thestandardphenol / chloroform protocol). Using DNA barcode primers to PCR amplify the mitochondrial Cytb gene fragments and sequence determination of takin, yak, and cattle, specifically comprising the following steps:

[0025] 1) The PCR reaction system is 45 μL, including: 1 μL of DNA template with a concentration of 50 ng, Mix 20 μL, 1.0 μL of upstream and downstream primers with a concentration of 10 pM, and add water to make up 45 μL of the system, in order to prevent the evaporation of the liquid in the PCR tube during the reaction , add 2.0 μL glycerol to the surface of the system; the reaction is completed on a MyCyclerThermalCycler therm...

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PUM

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Abstract

The invention discloses a DNA bar code primer for quickly identifying Budorcas taxicolor and also discloses a molecular biological method for quickly identifying Budorcas taxicolor. Total DNA of Budorcas taxicolor, Bos grunniens and Bos taurus domestica are extracted by using a standard phenol-chloroform method or a DNA extraction kit; mitochondrial Cytb sequences of Budorcas taxicolor, Bos grunniens and Bos taurus domestica are subjected to PCR (polymerase chain reaction) amplification and sequencing by using the DNA bar code primer to obtain markers; species identification is carried out according to sequence-specific sites of nucleotide of Budorcas taxicolor. Cytb gene sequences of mitochondrial DNA of Budorcas taxicolor are measured through DNA bar codes, namely amplification for the first time, thus identifying the three species; necessary molecular biological evidence is provided for effective protection of endangered species and effective struggle against illegal hunters.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular biological method for rapidly identifying takin. Background technique [0002] Takin (Budorcastaxicolor) is a first-class protected animal in my country. It inhabits high-altitude and alpine cliffs and has a large body. It is often the target of illegal poaching. When the forest police seized poached takinone bodies, they could not distinguish them from yaks and cattle by relying on traditional classification and morphological characteristics. Easy to confuse and make mistakes. Secondly, traditional identification methods are difficult to carry out the identification of disabled individuals. [0003] In recent years, DNA barcoding has provided new research methods and means for species classification and identification. DNA barcodes, also known as DNA barcodes, are similar to the black and white stripe patterns of different thicknesses and int...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 姚永芳徐怀亮倪庆永张明旺李地艳邬应龙解萌张钰林周言言桓宗锦
Owner SICHUAN AGRI UNIV
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